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FBO DAILY - FEDBIZOPPS ISSUE OF AUGUST 08, 2015 FBO #5006
DOCUMENT

66 -- ImageXpress Microscopic System or equal - Attachment

Notice Date

8/6/2015
 

Notice Type

Attachment
 

NAICS

333314 — Optical Instrument and Lens Manufacturing
 

Contracting Office

Department of Veterans Affairs;Program Contract Organization East(PCOE);323 North shore Dr;Pittsburgh, PA 15212
 

ZIP Code

15212
 

Solicitation Number

VA26815R0129
 

Response Due

8/11/2015
 

Archive Date

8/26/2015
 

Point of Contact

Contracting Officer: Michael Haydo
 

Small Business Set-Aside

Total Small Business
 

Description

This is a PRE-SOLICITATION NOTICE of a proposed requirement to award one (1) ImageXpress Microscopic Screening System or an item considered equal or better in accordance with the salient characteristics. The actual requirement will be posted for bids on the Federal Business Opportunities (FBO) as a Total Small Business (SB) Set-aside with a preference for Veteran Owned Small Businesses (VOSB) and Service-Disabled Veteran-Owned Small Businesses (SDVOSB). The Veteran's Health Administration Service Area Office - East estimates a posting date of solicitation VA268-15-R-0129 on Aug 14, 2015 and distributed solely through the FBO website (www.fedbizopps.gov). This notice is issued solely for informational purposed and does not constitute a Request for Proposal (RFP) and should not be construed to as a commitment of any kind by the Government to issue a formal solicitation or ultimately award a contract. Responses to this notice are not offers and cannot be accepted by the Government to form a binding contract. Any costs incurred by interested parties will NOT be reimbursed. It is the responsibility of any interested party to monitor this site for additional information pertaining to this notice. The agency is contemplating single award of a firm fixed price (FFP) contract to the contractor whose submission represents best value to the Government. The North American Industry Classification System code is 333314 Optical Instruments and Lens Manufacturing with a small business size standard of 500 employees. Interested parties shall be registered in the System for Award Management at (www.sam.gov). Any SB, SDVOSB, or VOSB that can provide an equal product shall submit their technical capabilities, to include technical specifications of their product by email to the point of contact below no later than August 11, 2015. Phone inquiries will not be accepted. Description of Requirement: The system shall have the flexibility to image monolayers of cultured cells as well as glass slides of sections of tissue. A wide selection of objectives (a minimum of 10x to 100x). The ability to image both fluorescent and bright-field samples, tissue samples examined for blood-vessel infiltration (immunofluorescence staining of markers of endothelial cells) as well as increased extracellular matrix formation (collagen expression as seen by Masson's trichrome staining). The system shall allow for exact quantitation of both the thickness of this matrix layer and blood vessel infiltration through software applications that allow for edge detection in the bright-field mode and spot recognition in the fluorescence mode. The system shall employ state-of-the-art motor positioning of the lens in the X-Y-Z axis with 100nm resolution to precisely locate and identify subcellular features. The system shall maintain a comprehensive suite of software applications to enable turnkey image segmentation and quantification tasks. The software application modules shall enable the use of a multitude of tasks such as: Cellular Screening - FLIPR membrane potential sodium channel modulation assays, measuring intracellular calcium flux, determination of cellular membrane potential, live cell G-protein coupled receptor second messenger signaling, cardiotoxicity assays using live beating cardio myocytes; High Content Imaging - measures of cytotoxicity, RNAi screening for DNA damage, evaluation of cell surface binding of antibodies, cell migration assays, total and phosphor-protein detection; Microplate Imaging - for stain free cellular image analysis without the use of fluorescent dyes, toxicity assays, measurements of cell marker expression. Instrumentation shall also meet the following specifications: 1.A fully-integrated, wide-field imaging system for automated acquisition and analysis of images for high throughput cell-based screening. 2.Be capable of producing a minimum image flexibility score (IFS) ? 466 (IFS = range of objective magnification range [1-100X] X sensor size (4.66 Mpixels). 3.Optically-encoded linear motors to accurately and precisely position in all three axes with better than 100nm resolution. 4.High-speed firmware-controlled auto-focus with a dedicated laser and fast CMOS detector to reliably focus on the sample and minimize bleaching. Be capable of both laser and image-based autofocus as software configurable options. 5.Utilize custom designed optics for optimal illumination and signal detection. 6.Enable adjustable field-of-view focus on the most appropriate sample area. 7.Contain a software-controlled linear objective changer which protects unselected objectives from contact with the stage. 8.Permit adaptive acquisition for on the fly cell counting to normalize field of view capture to target cell count. 9.Permit the option of using either bright-field or true phase contrast imaging with transmitted light. 10.Support the use of colorimetric dyes such as Oil Red O and hematoxylin and eosin. 11.Be compatible with a wide variety of multi-well plate types and slides including both thick and thin, glass or plastic, and trans-well formats. 12.Utilize user changeable objectives and filters to maximize system flexibility. 13.Be capable of screening ? 12 million cells/hour for a 384-well, 3 color assay, or ? 2300 wells/hour for a 2 color high resolution 1536 well plate. 14.Be equipped with a 16-bit CMOS camera as a sensor to enable 4.66 megapixel captures, more than 3 log dynamic range intensity detection, and 16 bit digitization. 15.Be equipped with an environmental control chamber which regulates temperature, CO2 level, and humidity level to maintain cells in an incubator-like environment. 16.Utilize a flexible light source to match the range of assay needs across a wide spectra from UV to near IR wavelengths. 17.The image acquisition detector can be configured in the software to limit the percentage of the camera chip used for acquisition. 18.Incorporate a cooled 16-bit monochrome sCMOS camera with 2160 X 2160 imaging array capable of 100 fps and with low noise (1.2 e-/pixel at slow digitization) leading to 1:25,000 dynamic range. 19.Image processing conducted using software which controls both image acquisition and analysis and which offers optional application modules to easily automate common image analysis tasks. Software must enable the use of application modules which feature intuitive dialog boxes for set-up of image analysis, improved segmentation through adaptation to local content, image and cell-by-cell data logging in typical applications which include but are not limited to: a.Apoptosis b.Endothelial tube formation c.Promotion or inhibition of blood vessel formation d.Cell viability e.Cell cycle analysis f.Membrane potential g.Cytotoxicity h.Cell proliferation i.Determination of cell health and proliferation in populations of mono-, bi-, and multi-nucleated cells j.Micronuclei analysis for genotoxicity k.Cell migration l.Cell counting m. Kinase activation n. Fatty acid uptake o.Mitosis p.Adipogenesis q.Examining transfection efficiencies r.Studying intracellular structures s.Receptor internalization t.Punctate staining u.Clustering target molecules v.Process extension w.Neurodegenerative disease research x. Cell differentiation y.Protein movement, translocation, and co-localization z.Cell pathway analysis aa.Protein phosphorylation bb. Mitochondrial localization and toxicity cc.Detection of monopolar spindles dd.Angiogenesis tube formation ee.Time-lapse and kinetic analysis Contracting Office Address: 323 North Shore Dr. Pittsburgh PA, 15212 United States Primary Point of Contact: Michael Haydo Contracting Officer Email: michael.haydo@va.gov Phone: 412-822-3158
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/notices/e9176f40f8e9529ab90b1df16e748313)
 

Document(s)

Attachment
 

File Name: VA268-15-R-0129 VA268-15-R-0129_1.docx (https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=2224227&FileName=VA268-15-R-0129-000.docx)

Link: https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=2224227&FileName=VA268-15-R-0129-000.docx

 

Note: If links are broken, refer to Point of Contact above or contact the FBO Help Desk at 877-472-3779.
 

Place of Performance

Address: Ralph H. Johnson Medcial center;Charleston, SC

Zip Code: 29403
 

Record

SN03827985-W 20150808/150807000323-e9176f40f8e9529ab90b1df16e748313 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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