SOLICITATION NOTICE
65 -- NEBNext Reagents- Brand Name or Equal
- Notice Date
- 8/7/2015
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 325414
— Biological Product (except Diagnostic) Manufacturing
- Contracting Office
- US Army Medical Research Acquisition Activity, ATTN: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014
- ZIP Code
- 21702-5014
- Solicitation Number
- W81XWH-15-T-0316
- Response Due
- 8/18/2015
- Archive Date
- 10/6/2015
- Point of Contact
- Darrell Beaver, 301-619-4019
- E-Mail Address
-
US Army Medical Research Acquisition Activity
(darrell.l.beaver4.civ@mail.mil)
- Small Business Set-Aside
- N/A
- Description
- This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. Minimum Essential Characteristics (Brand Name or Equal) All items proposed must have a minimum of 6 months until expiration of product. NEBNext Ultra DNA Library Prep Kit for Illumina (96 rxn, Manf#: E7370L): (Brand Name or Equal) A kit which uses enzymes and buffers to convert a small amount (low quantity) of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). Shall have input amounts of DNA required must be as low as 5ng. Each set of reagents shall be functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform (Illumina, Inc.). Kit shall exhibit ultra-high fidelity amplification with minimized GC bias. Shall be compatible with NEBNext Multiplex Oligos for Illumina (Dual Index Primer Set 1). Shall be compatible NEBNext High-Fidelity 2X PCR Master Mix. NEBNext Multiplex Oligos for Illumina (Dual Index Primer Set 1), 96 rxn (Manf# E7600S): (Brand Name or Equal) Required reagents for NEBNext Ultra DNA Library Prep Kit for Illumina platform (Illumina, Inc). Shall contain adaptors and primers compatible with NEBNext kits for multiplex sample preparation for next-generation sequencing on the Illumina platform (Illumina, Inc.). Shall contain index primers set 1. Each set of reagents shall be functionally validated together through construction and sequencing of a DNA library on the Illumina sequencing platform (Illumina, Inc.). Individual reagents shall undergo standard enzyme activity and quality control assays. NEBNext High-Fidelity 2X PCR Master Mix, 250rxn, (Manf# M0541L): (Brand Name or Equal) Required reagents for NEBNext Ultra DNA Library Prep Kit for Illumina platform (Illumina, Inc.). Shall contain buffer specifically optimized for the robust, high-fidelity amplification of next-generation sequencing libraries on the Illumina platform (Illumina, Inc), regardless of GC content. Shall contain High-Fidelity DNA Polymerase that possesses 3' 5' exonuclease activity fused to a processivity-enhancing Sso7d domain. Shall contain High-Fidelity DNA Polymerase that has an ultra-low error rate ( greater than 100-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase). Shall contain dNTPs, Mg++ and buffer, and require only the addition of primers and DNA template for amplification. Shall generate PCR products with blunt ends. PROPOSAL DUE DATE: No Later Than (NLT): 18 August 2015 at 4:00 PM Eastern Time. Price quotes must be emailed to Darrell Beaver at darrell.l.beaver4.civ@mail.mil.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USA/USAMRAA/DAMD17/W81XWH-15-T-0316/listing.html)
- Place of Performance
- Address: Armed Forces Medical Examiners System (AFMES) 115 PURPLE HEART DRIVE DOVER AIR FORCE BASE DE
- Zip Code: 19902
- Zip Code: 19902
- Record
- SN03829566-W 20150809/150807235526-54c0ce146c2e219294270c90d316a77c (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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