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FBO DAILY - FEDBIZOPPS ISSUE OF JANUARY 15, 2016 FBO #5166
SOURCES SOUGHT

R -- Deep interactome profiling of the Tmem16A Cl channel protein by Co-interacting Protein Identification Technology (CoPIT)

Notice Date

1/13/2016
 

Notice Type

Sources Sought
 

NAICS

541711 — Research and Development in Biotechnology
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
 

ZIP Code

20892-7902
 

Solicitation Number

NHLBI-CSB-DE-2016-063-KEW
 

Archive Date

2/11/2016
 

Point of Contact

Kyle Wisor, Phone: 3014023670, Rashida Ferebee,
 

E-Mail Address

Kyle.Wisor@nih.gov,
(Kyle.Wisor@nih.gov, ontract)
 

Small Business Set-Aside

N/A
 

Description

A. Sources Sought Notice: NHLBI-CSB-DE-2016-063-KEW B. Title: Deep interactome profiling of the Tmem16A Cl channel protein by Co- interacting Protein Identification Technology (CoPIT) C. This is a Sources Sought Notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified business sources; and (2) their size classification relative to the North American Industry Classification System (NAICS) code 541711 for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method. All capability Statements sent in response to this Small Business Sources Sought notice must be submitted electronically (via email) to Kyle Wisor, Contract Specialist, at kyle.wisor@nih.gov in either MS Word or Adobe Portable Document Format (PDF), by January 27th, 2016 9:00 AM, EASTERN TIME under Solicitation Number: NHLBI-CSB-DE-2016-063-KEW. FAXES ARE NOT ACCEPTED D. Information: 1. Background The National Institutes of Health (NIH) is the nation's leading medical research agency and the primary Federal agency conducting and supporting medical discoveries that improve people's health and save lives. The mission of the National Institute of Dental and Craniofacial Research (NIDCR) is to improve oral, dental and craniofacial health through research, research training, and the dissemination of health information. The Melvin Laboratory, Secretory Mechanisms and Dysfunction Section (SMDS), NIDCR/NIH, and the Yates Laboratory at The Scripps Research Institute (SRI) have a long-standing scientific collaboration over the past decade. The physiology expertise of the Melvin Laboratory and the development of cutting-edge proteomic methods by the Yates Laboratory have been combined to characterize novel aspects of salivary gland function. This collaboration has resulted in nine joint publications to date. It is estimated that one out of every four or five persons will suffer from xerostomia (dry mouth) during their lifetime. The clinical costs of dry mouth include mucosal infections and ulcers, altered taste, dysphagia and pain. To develop specific and effective interventions to treat dry mouth it is necessary to understand the molecular nature of the fluid secretion process. Acinar cell fluid secretion is driven by transcellular Cl movement. The Melvin Laboratory has recently shown that activation of an apical Ca2+-dependent Cl channel encoded by the Tmem16A gene (Catalán et al., 2015) is the obligatory, rate-limiting step in fluid production. Although it is clear that Ca2+ directly activates Tmem16A, it is unknown how sustained activity of the Tmem16A channel is maintained/regulated. 2. Purpose and Objectives The SMDS, in collaboration with the contractor, will develop antibody-based protein enrichment methods to isolate the plasma membrane-associated Tmem16A channel proteins from acutely isolated native mouse acinar cells, followed by mass spectrometry analysis by the contractor. 3. Project Requirements Contractor Tasks: The below tasks are to be executed sequentially. [1] The contractor and the NIDCR shall develop a customized experimental procedure that addresses several issues associated with enrichment of membrane protein interactomes: (a) highly efficient recovery of membrane proteins from cell lysates while preserving interactions, (b) insolubility and aggregation of membrane proteins during IP and subsequent elution, (c) removal of lipids and other contaminants that may cause signal suppression during electrospray ionization, and (d) compatibility with in-solution digestion, chromatographic separation and mass spectrometric detection; [2] The contractor shall use MUDPiT (multidimensional protein identification technology) to determine the molecular identifies of the isolated proteins and simultaneously acquires the PTM (post-translation modification) state of these proteins; [3] The contractor shall use CoPIT (Co-interacting Protein Identification Technology) to determine the specificity of protein interactions in comparison to control experiments for label-free data by using novel methods for unbiased subtraction of background interactions; [4] The contractor and the NIDCR shall use the interactomes from individual experimental conditions to compare against each other to yield differential interactomes based on spectral counting. The Radial Topology Viewer, a visualization tool, graphs interactors, whereby the arithmetic distance to the bait in the center can reflect a dynamic variable of the user's choice, for example confidence of interaction or change in strength of interaction. Additional relational information on protein-protein interactions gathered from other databases can be included to enable the contextual interpretation of a protein interactome; [5] The contractor and the NIDCR shall confirm the significance of protein interactomes and PTMs identified above by co-expression and mutational functional analysis, respectively. 4. Deliverables [1] March 14, 2016: Complete the development and use of the customized experimental procedures for efficient recovery of membrane proteins from cell lysates while preserving interactions; [2] June 14, 2016: MUDPiT and CoPIT analyses will be performed, i.e. the molecular identifies of the isolated proteins and the specificity of protein interactions will be completed; [3] September 14, 2016: Interactomes from individual experimental conditions will be compared against each other to yield differential interactomes based on spectral counting; [4] December 14, 2016: The functional significance of protein interactomes will be confirmed by co-expression and mutational analysis; [5] Interim progess reports will be provided upon request or following a milestone, whichever comes first. 5. Contractor Requirements a. The contractor will use MUDPiT and CoPIT methodologies developed by the Yates Laboratory. The methods are a combination of customized hardware and unique analyses software. Note that these methods are not available anywhere else in the world. 6. Government Requirements a. The NIDCR will work in conjunction with the contractor to develop a customized experimental procedure, analyze MUDPiT and CoPIT results, and perform experiments to test the significance of protein interactomes and PTMs by co-expression and mutational functional analysis. 7. Anticipated Period of Performance/Delivery Date Base Year: February 2016 - February 2017 Option Period 1: February 2017 - July 2017 Performance Location National Institutes of Health, NIDCR 10 Center Drive Building 10 Bethesda, MD 20892 8. Capability Statement Interested parties are expected to review this notice to familiarize itself with the requirements of this project. Failure to do so will be at your firm's own risk. The following information shall be included in the capability statement: a. A general overview of the respondents' opinions about the difficulty and /or feasibility of the potential requirement, and any information regarding innovative ideas or concepts. b. Information as needed in sufficient details of the respondents' (a) staff expertise, including their availability, experience, and formal and other training; (b) current in-house capability and capacity to perform the work; (c) prior completed projects of similar nature; (d) corporate experience and management capability; and (e) examples of prior completed Government contracts, references, and other related information. c. The respondents' DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HUBZONE, etc) pursuant to the North American Industry Classification System (NAICS) code: 541711, Research and Development in Biotechnology, Small Business Size 500 employees. d. Any other information that may be helpful in developing or finalizing the requirements of the potential acquisition. e. The capability statement shall not exceed 20 single-sided pages (including all attachments, resumes, charts, etc.) presented in single-space and using a 12-point font size minimum, in either Microsoft Word or Adobe Portable Document Format (PDF), with 8-1/2 by 11 inch paper size, and 1 inch top, bottom, left and right margins. f. All proprietary information should be marked as such. Statements should also include an indication of current certified small business status; this indication should be clearly marked on the first page of your capability statement (preferably placed under the eligible small business concern's name and address). Responses will be reviewed only by NIH personnel and will be held in a confidential manner. 9. Closing Statement All capability Statements sent in response to this Sources Sought Notice must be submitted electronically (via email) to Kyle Wisor, Contract Specialist, at kyle.wisor@nih.gov in either MS Word or Adobe Portable Document Format (PDF), by January 27th, 2016 9:00 AM, EASTERN TIME under Solicitation Number: NHLBI-CSB-DE-2016-063-KEW. FAXES ARE NOT ACCEPTED. This Sources Sought Notice is for information and planning purposes only and shall not be construed as a solicitation or as an obligation on the part of the National Heart, Lung, and Blood Institute (NHLBI). The NHLBI does not intend to award a contract on the basis of responses nor otherwise pay for the preparation of any information submitted. As a result of this notice, the NHLBI may issue a Request for Quote (RFQ). THERE IS NO SOLICITATION AVAILABLE AT THIS TIME. However, should such a requirement materialize, no basis for claims against NHLBI shall arise as a result of a response to this notice or the NHLBI's use of such information as either part of our evaluation process or in developing specifications for any subsequent requirement. "Disclaimer and Important Notes. This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)."
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-DE-2016-063-KEW/listing.html)
 

Place of Performance

Address: National Institutes of Health, NIDCR, 10 Center Drive, Building 10, Bethesda, Maryland, 20892, United States

Zip Code: 20892
 

Record

SN03990745-W 20160115/160113235023-7b5487a0cc87a2f56414c175c0a1f493 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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