SOURCES SOUGHT
65 -- Oligos-Bar coded primers - Oligo-Bar coded primers RFI
- Notice Date
- 7/11/2016
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of the Army, U.S. Army Medical Research Acquisition Activity, U.S. Army Medical Research Acquisition Activity, Attn: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014, Maryland, 21702-5014, United States
- ZIP Code
- 21702-5014
- Solicitation Number
- W81XWH16T0319
- Archive Date
- 7/29/2016
- Point of Contact
- Hugo M. Robinson, Phone: 3016197811
- E-Mail Address
-
hugo.m.robinson.civ@mail.mil
(hugo.m.robinson.civ@mail.mil)
- Small Business Set-Aside
- N/A
- Description
- PWS MECs Misc info requested Request for Information (RFI) Oligos-Bar coded primers General Information: This is an RFI as defined in FAR 15.201(e) to ascertain whether a commercial item exists that provides the capabilities described herein. This RFI is issued solely for information and planning purposes and does not constitute a solicitation. All information received in response to this RFI will be treated as business confidential information. Nonetheless, submitters should properly mark their responses if the information is confidential. Responses to the RFI will not be returned. Responses to this notice are not offers and cannot be accepted by the Government to form a binding contract. Responders are solely responsible for all expense associated with responding to this RFI. RFI Response and due date: COB 14 July 2016 Instructions to Responders: Responses from all capable and qualified sources are encouraged to respond to this request. White papers should adhere to the following formatting and outline instructions: 1. All submissions shall be made electronically and be in one of the following formats: Microsoft Word or Adobe PDF. 2. Cover Page (1page) • Title • Organization • Responders technical and administrative points of contact (names, addresses, phone, fax number and email addresses) • Topic area(s) addressed 3. Completed Attachment 1 and Attachment 2. Please note that the response shall provide sufficient technical data to determine how you fully meet the requirements as specified in Attachment 2. Please note that responses are due no later than COB 14 July 2016. The preferred method of submission is via email. The point of contact for this action is Hugo Only electronic responses are requested. No evaluation letters and/or results will be issued to the respondents. At this time no solicitation exists; therefore, please do not request a copy of the solicitation. NO PHONE CALLS PLEASE. It is the responsibility of any potential offeror to monitor this site for the release of any solicitation or synopsis. Background/Overview: The Walter Reed Army Institute of Research (WRAIR) in Silver Spring, MD hosts a central repository of multi-drug resistant organisms (MDROs). Isolates sent to the WRAIR from Department of Defense (DOD) Medical Treatment Facilities (MTFs) and other non-DOD MTFs throughout the world. On Sept 18th, 2014, the President of the United States announced a mandate to create an inter-agency Combating Antibiotic Resistant-Bacteria (CARB) task force. According to this mandate, government agencies must catalog and test MDROs for antibiotic resistance patterns and ascertain as much related information, such as mechanism of resistance and types of carried resistance genes, as possible. This is best accomplished through high-throughput sequencing of individual MDRO isolates. An essential item for sequencing includes the specialized exogenously added oligonucleotides (aka "primers") with identifiable nucleic acid sequences, or "barcodes" added to start the sequencing synthesis process. MINIMUM ESSENTIAL CHARACTERISTICS Barcoded Primers 1. Primers (aka oligonucleotides) shall contain a phosphorylated 5' (five-prime) modification. 2. Primers shall contain a 3' (three prime) phosphorothioate modification. 3. Primers shall be manufactured on a 1 umole synthesis scale. 4. HPLC or PAGE purification options shall be available during synthesis (HPLC is sufficient). 5. Primers shall be either lyophilized or an acceptable working solution (i.e. Tris pH 8.0 buffer; water or TE buffer is not acceptable) that is adequate for long term freezer storage. 6. Vendor shall be able to provide primers containing 65-70 base pairs. 7. Vendor shall take synthesis orders on-line. 8. Vendors shall be able to synthesize a sufficient number of primers to result in 384 unique barcode reads (i.e. minimum 40 primers) to be synthesized, purified, and shipped within 14 business days of receipt of order. 9. Primers shall be supplied in individual tubes or on a plate with removable individual tubes/wells. MRSN/WRAIR; Barcoded Primer Performance Work Statement INTRODUCTION: The Walter Reed Army Institute of Research (WRAIR) in Silver Spring, MD hosts a central repository of multi-drug resistant organisms (MDROs). Isolates sent to the WRAIR from Department of Defense (DOD) Medical Treatment Facilities (MTFs) and other non-DOD MTFs throughout the world. On Sept 18th, 2014, the President of the United States announced a mandate to create an inter-agency Combating Antibiotic Resistant-Bacteria (CARB) task force. According to this mandate, government agencies must catalog and test MDROs for antibiotic resistance patterns and ascertain as much related information, such as mechanism of resistance and types of carried resistance genes, as possible. This is best accomplished through high-throughput sequencing of individual MDRO isolates. An essential item for sequencing includes the specialized exogenously added oligonucleotides (aka "primers") with identifiable nucleic acid sequences, or "barcodes" added to start the sequencing synthesis process. BACKGROUND: The mission of the MRSN is to conduct epidemiological surveillance of MDROs to enhance performance improvement and infection control across the DOD. Recently, a mandate to create an inter-agency Combating Antibiotic Resistant-Bacteria (CARB) task force was established with the goal of inter-agency sequencing data standardization and sharing. As such, the number of MDROs submitted to the MRSN has increased along with the expectation of information output (i.e. sequencing data). To initiate the sequencing reaction synthetically produced oligonucleotides, known as primers, are added to the reaction mixture. These primers must have a unique sequence or "barcode" which is copied over to the end synthesis product and allows parsing of the products into unique individual DNA strands for downstream data collection. Barcoded oligonucleotides are essential and without them, no sequencing reaction can be performed. SCOPE OF WORK: WRAIR intends to initiate a blanket purchase agreement for barcoded primers. These primers must contain unique sequences for barcoding purposes, contain a phosphorylated 5' (five-prime) modification, a 3' phosphorothioate modification, be HPLC purified, and lyophilized for long term storage. Additionally, the vendor must be able to synthesize primers on a 1 umole scale and provide primers containing 65-70 base pairs per oligo and take synthesis orders on-line with a production and shipping time not to exceed 5 days from order placement. Product Description/Quantity HPLC Purified-1umole scale-70bp oligo w/ 3' phosphorothioate bond; Qty: 240 HPLC Purified-1umole scale-65bp oligo w/ 5' phosphate modification; Qty: 360
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USA/USAMRAA/DAMD17/W81XWH16T0319/listing.html)
- Place of Performance
- Address: Walter Reed Army Institute of Research (WRAIR), Silver Spring, Maryland, 20910, United States
- Zip Code: 20910
- Zip Code: 20910
- Record
- SN04178097-W 20160713/160711235046-7c048af8f9180c4a17548c02e3d1c94d (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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