SOURCES SOUGHT
66 -- Total Internal Reflection Fluorescence Microscope System (Inverted)
- Notice Date
- 8/5/2016
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-SBSS-16-641
- Archive Date
- 8/26/2016
- Point of Contact
- Lauren M. Phelps, Phone: 3015942490
- E-Mail Address
-
lauren.phelps@nih.gov
(lauren.phelps@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Sources Sought Notice/Request for Information Total Internal Reflection Fluorescence Microscope System (Inverted) HHS-NIH-NIDA-SSSA-SBSS-16-641 Contracting Office Address: Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, MD 20892, UNITED STATES. Introduction: This is a Sources Sought Notice/Request for Information for Market Research purposes. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified large and small business sources, (2) whether they are small businesses; HUBZone small businesses, service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. Background: The Membrane Transport Biophysics Section (MTBS) of the National Institute of Neurological Disorders and Stroke (NINDS) studies the functions of transporter proteins in cellular membranes and their biological roles. A major focus of MTBS work is the understanding of the role of chloride transport in subcellular organelles, including lysosomes. The Section has identified a chloride transporting protein, ClC-7, which is involved in the mechanisms by which these organelles generate an acidic interior. The Section has been studying the action of ClC-7 using a variety of approaches, including biochemical analysis and microscopy, but for new experimental directions, requires the ability to assess the acidification of individual lysosomes isolated from living cells. To do these experiments requires a specialized microscope to visualize the individual lysosomes using Total Internal Reflection Fluorescence microscopy (TIRF). Purpose and Objectives for the Procurement: The purpose of this requirement is to acquire one (1) Total Internal Reflection Fluorescence microscope system (inverted) to be used to capture high-quality images of live cells and individual isolated lysosomes for studies of lysosomal function and dysfunction. Project Requirements: The NINDS MTBS requires one (1) fully motorized TIRF microscope system (inverted) that shall have a turn-key solution for hardware-driven sequencing of the activity of excitation lasers, x-y-z axis movements, filter wheels, and cameras. The equipment shall meet the following requirements: A.The system must be based on a fully motorized inverted microscope stand with motorized control of extra-fine/fine/coarse focus, filter cube changing, escape/refocus mechanism, light distribution, and motorized shutters for each light source. The body must also contain an intermediate magnification changer with 1.5x, left and right sideports for cameras, and 10x eyepieces. B.The system must have a 100W halogen lamp with appropriate power supply and condenser turret for transmitted light illumination with an appropriate high transmission daylight colorbalance filter. C.The system must have 4x, 10x, and 40 x high quality (plan fluor) objective lenses, and a numerical aperture (NA 1.49) apochromat 60x oil immersion lens with a correction collar for TIRF imaging. All objective lenses should be equipped with appropriate prisms for Differential Interference Contrast imaging. D.The system must have a motorized X-Y scanning stage with linear encoders and an integrated encoded motorized z axis drive. Interactive control of stage axes, filter changers, shutters, focus, and reflector changers is required. E.The systme must come with holders for 55x25 mm Labtek chambered coverslips, 35 mm Petri Dishes, or 1x3" microscope slides. F.The system must have an LED-based white-light illumination system for wide-field fluorescence and filter cubes for DAPI, FITC, TRITC, and Cy5. G.The system must include lasers for objective-based TIRF illumination and alaser launch for TIRF. Lasers must include lasers with wavelengths of 445, 488 561 and 640 nm (20 mW each at the microscope) and be capable of supporting up to three more lasers in the future. H.The system must utilize a Hammamatsu ORCA Flash4.0 v2+ sCMOS camera with USB 3.0 interface to achieve maximum field of view and sensitivity with 82% quantum efficiency. I.The system must include a 64 bit imaging workstation computer. J.The system must include a real-time Z drift correction system that maintains a fixed focal plane setting in the settings of potential thermal drift and uneven plate surfaces. This drift correction mechanism should actively track the axial location of the sample vessel up to 20 times per second. K.The system must include software that enables full computerized control of all microscope features and image acquisition. The software should include abilities for multidimensional acquisition, (including XY, Z, T and large image tiling), real-time deconvolution, ratio imaging module specifically designed for real-time ratiometric imaging and calculating FRET efficiency. L.The system must be able to image cells in pre-specified regions and/or wells of multiwall dishes and perform post-acquisition segmentation analysis and quantification of the resulting images in an automated fashion. M.The system must include anair isolation table with optical top. N.The system should utilize infinity corrected optics that allow for insertion of additional optical tiers between the objective back aperture and microscope tube lens. Each optical tier should be capable of integrating multiple illuminators for maximum flexibility of excitation wavelength combinations and imaging modalities. O.A port unit should be available for the system, which allows a camera or excitation illuminator to be mounted for specialized photostimulation or emission splitting applications, such as FRET or ratiometric imaging. P.The software included with the microscope system must include auto-drift correction, supplemental image contrast based auto focusing, remote control over all motorized components of the microscope system, ratio-metric imaging and ratio calculation, full 3D volume rendering, time measurement for live and post-acquisition recording of image and ROI-based intensity analysis and live ratio imaging, binary operation functions including image segmentation and pixel classification, automated binary and ROI measurement functions, multidimensional acquisition for freely combining multiposition, timelapse Z stacking and multicolor imaging with a high degree of flexibility, and a diverse and accessible macro control language for programming custom functions into acquisition routines. Training Requirements: On-site training must take place at the customer site within two (2) weeks of equipment installation. Training instruct users on the operation of the instrument and shall be for up to five (5) individuals over a two (2) day period for duration of at least eight (8) hours per day. Training shall take place between the hours of 8:00am and 5:00pm, Bethesda, MD local prevailing time, Monday through Friday. Warranty Requirements: The Contractor shall provide at a minimum, a one (1) year warranty on all equipment, supplies and repairs. Contractor shall provide technical support service during this period. Technical support service shall be on-site and/or virtual and include technical assistance and training on all service related issues. All products shall be warranted to be free from material defects in workmanship or material under normal use and service for warranty period. Anticipated Contract Type: A firm fixed price contract is anticipated. Invoicing is anticipated to be made on a NET30 basis and payment via Electronic Funds Transfer (EFT) is contemplated. Anticipated Period of Performance: The anticipated contract period of performance is fourteen (14) weeks from date of award. The contractor shall deliver and install the required equipment within twelve (12) weeks after receipt of order. Delivery must be FOB Destination and must include inside installation. The equipment shall be delivered and installed between the hours of 8:00am and 5:00pm, Bethesda, MD local prevailing time, Monday through Friday. Onsite training must take place within two (2) weeks following installation. The contractor shall provide a company certified service technician to perform setup, installation and training. Capability Statement: Contractors that believe they possess the ability to provide the required commercial microscope equipment should submit documentation of their ability to meet each of the project requirements to the Contract Specialist. The capability statement should include 1) the total number of contractor employees, 2) documentation of ability to provide the required system, 3) any contractor GSA Schedule contract(s) by which all of the requirements may be met, and 4) any other information considered relevant to this potential requirement. Contractors must also provide their Company Name, DUNS number, Physical Address, and Point of Contact Information. Interested organizations are required to identify their type of business, applicable North American Industry Classification System Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number. All capability statements sent in response to this Sources Sought Notice must be submitted electronically to Lauren Phelps, Contract Specialist (Lauren.Phelps@nih.gov) before the closing date and time of this announcement. All responses must be received by the specified due date and time in order to be considered. Note: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in the response. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After review of the responses received, pre-solicitation and solicitation notices may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. The solicitation release date is pending. The Government anticipates negotiation of a contract.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-2/HHS-NIH-NIDA-SSSA-SBSS-16-641/listing.html)
- Place of Performance
- Address: Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN04211407-W 20160807/160805234520-c4ec3159acaaf170c869ede463323a6f (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |