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FBO DAILY - FEDBIZOPPS ISSUE OF SEPTEMBER 11, 2016 FBO #5406
SOLICITATION NOTICE

66 -- Gradient Fractionation System - Combined Synopsis Solicitation 4246217

Notice Date

9/9/2016
 

Notice Type

Combined Synopsis/Solicitation
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 3155, MSC 9593, Bethesda, Maryland, 20892, United States
 

ZIP Code

20892
 

Solicitation Number

HHS-NIH-NIDA-SSSA-CSS-713
 

Archive Date

9/28/2016
 

Point of Contact

Danielle R. Brown, Phone: 301 480 2385, Rachelle Trice, Phone: 301 594 1928
 

E-Mail Address

danielle.brown2@nih.gov, rachelle.trice@nih.gov
(danielle.brown2@nih.gov, rachelle.trice@nih.gov)
 

Small Business Set-Aside

Total Small Business
 

Description

Combined Synopsis Solicitation 4246217 The purpose of this purchase is to upgrade the Density Gradient Fractionation System used to analyze polysome profiles and protein translation in the Lab of Genetics. The system produces a continuous absorbance profile as the gradient is displaced from the bottom up via a syringe pump and tube piercing system, then collected in precisely measured fractions. The system includes the tube piercing system, syringe pump, UA-6 detector with 254 and 280 nm filters, fraction collector, density gradient flow cell, cables and tubing. The data acquisition software captures the real time data, and records the traces to be saved and analyzed digitally. Density gradient centrifugation is used for the gentle isolation of cellular ribosomes, RNA, DNA, and organelles. These unique density gradient systems automate the preparation of gradients, and fractionation and UV scanning of zones after the spin. Our current gradient fractionation system is heavily used, one to three times a week, for polysome fractionation of cell or tissue lysates centrifuged through linear sucrose gradients, then fractionated with this system while generating a continuous absorbance profile measuring the RNA content of each fraction. This polysome fractionation technique has been utilized in collaborations with multiple laboratories within NIA and also with collaborators around the world. The currently utilized fracitonation system was purchased in 2001. The data acquisition is direct recording onto paper, so if the recoridng device malfunctions or breaks, the sample profile is not recorded. Since the original purchase, the data acquisition software has become available, but this software requires upgrades to the original fractionation system. As the system is so widely used for NIA research analyses, both directly and through collaborative efforts, an upgraded fractionation system with digital data capture software will be much more efficient and productive for the Institute.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/HHS-NIH-NIDA-SSSA-CSS-713/listing.html)
 

Place of Performance

Address: 251 Bayview Boulevard, Baltimore, Maryland, 21224, United States

Zip Code: 21224
 

Record

SN04265868-W 20160911/160911093423-bf51337bb5d6625359c62e1e1ffec902 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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