SOLICITATION NOTICE
B -- serum analysis for porcine epidemic diarrhea virus - SOLE SOURCE POSTING
- Notice Date
- 1/30/2017
- Notice Type
- Justification and Approval (J&A)
- NAICS
- 541940
— Veterinary Services
- Contracting Office
- Department of Agriculture, Animal and Plant Health Inspection Service, MRPBS, ASD, Specialized Contracting Branch, 2150 Centre Avenue Building B, MS2E6, Fort Collins, Colorado, 80526, United States
- ZIP Code
- 80526
- Solicitation Number
- AG-6395-S-17-836518
- Archive Date
- 3/8/2017
- Point of Contact
- Todd K. Bierl, Phone: 6123363465
- E-Mail Address
-
Todd.K.Bierl@aphis.usda.gov
(Todd.K.Bierl@aphis.usda.gov)
- Small Business Set-Aside
- N/A
- Award Number
- AG-6395-S-836518
- Award Date
- 2/6/2017
- Description
- SOLE SOURCE J&A Serum samples collected from feral swine will be tested for exposure to porcine epidemic diarrhea virus (PEDV) using a whole virus ELISA developed at Iowa State University. Serum samples are diluted 1:50 and plates are loaded with 100 μl of the diluted sample per well. Plates are incubated at 25 °C (serum) for 1 h and then washed 5 times with PBS (1X pH 7.4). Positive and negative plate controls, i.e., antibody-positive and -negative experimental serum samples, are run in duplicate on each ELISA plate. The antibody response in serum and oral fluid samples is represented as sample-to-positive (S/P) ratios typical of ELISAs. A U.S. prototype PEDV isolate is used in the PEDV WV antibody ELISA. Each batch of approximately 1000 mL of PEDV is propagated on Vero cells. Results will be a positive or negative finding for each serum sample.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USDA/APHIS/MRPBS/AG-6395-S-17-836518/listing.html)
- Place of Performance
- Address: National Wildlife Disease Program, USDA, APHIS, WS, 4101 LaPorte Ave., Fort Collins, Colorado, 80521, United States
- Zip Code: 80521
- Zip Code: 80521
- Record
- SN04384971-W 20170201/170130234220-32185b3a9deda1d698aa671759c31fc3 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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