SOURCES SOUGHT
66 -- Two Photon Microscop
- Notice Date
- 4/14/2017
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-SBSS-17-319
- Archive Date
- 5/13/2017
- Point of Contact
- Lauren M. Phelps, Phone: 3014802453
- E-Mail Address
-
lauren.phelps@nih.gov
(lauren.phelps@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Federal Business Opportunities (FBO) SOURCES SOUGHT NOTICE 1.Solicitation Number: HHS-NIH-NIDA-SSSA-SBSS-17-319 2.Title: Two Photon Microscope 3.Classification Code: 66 4.NAICS Code: 334516 5.Description: This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources both large and small to perform a potential requirement. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Background: The NINDS Synaptic Physiology Section explores the dynamics and modulation of transmitter release, diffusion, and receptor activation at excitatory and inhibitory synapses in the mammalian central nervous system (CNS). Investigators study the biophysics of synaptic transmission and mechanism by which neurons integrate synaptic information. In many retinal neurons, key information processing occurs in processes that are distant from the cell body, such that cannot be detected by a somatic electrode. The study of such neurons benefits particularly from recent optical techniques that employ florescent sensors to measure changes in the membrane potential or calcium in remote areas in the cell. Providing investigators access to a state-of-the-art photon laser scanning microscope system is crucial to the mission of the NINDS and the Synaptic Physiology Section. Purpose and Objectives: The purpose of this requirement is to acquire one (1) two-photon laser scanning microscope system to examine the mechanisms of synaptic integration and dendritic information in amacrine and ganglion cells in the mouse retina. Project requirements: The NINDS Synaptic Physiology Section requires one (1) two-photon laser scanning microscope system that meets the following requirements: A.The system must employ either resonant scanning (8kHz) in the X axis with galvanometer-based scanning in the Y axis, or galvanometer-based scanning in X and Y axes with the capability to add resonance scanning at a later date. The system also must be equipped with automated z-axis movement to enable automated acquisition of XYZ stacks. Alignment tools must be included. B.The system must include two GaAsp photomultiplier tubes (PMTs) for sensitive, two-channel fluorescence detection and filters that enable imultaneous detection of red and green fluorescences.The system must possess or be upgradable to PMTs with rapid gating capabilities (e.g., Hamamatsu H11706P-40) to enable simultaneous fluorescence detection and visible light stimulation. Preamp electronics must be enclosed within the PMT casing or otherwise stored to minimize excess wiring. C.The system must have a minimum of 20X / 1.0 NA objective, with a minimum 2.0 mm working distance that should exhibit minimal auto fluorescence/phosphorescence upon exposure to Ultraviolet (UV) light. D.The system must have an X-Y scanning stage, motorized z-axis objective positioning and a condenser for transmitted light imaging. Either Dodt or DIC optics must be included for transmitted infrared contrast imaging. E.The system must include a CCD camera for transmitted light imaging. F.The system must include a Pockel cell, driver and mount for rapid laser attenuation. G.The system must allow for user installed patterned (spatial) visual light stimulation, through either the objective or condenser lenses. The optics must be compatible with UV (365-405 nm) stimulus light and make it possible to deliver UV light stimulus without encountering significant stimulus-related noise (due either to spectral leak or to auto fluorescence/phosphorescence) in the green or red emission detection channels. H.The system must include a PC work station, peripherals and software compatible with galvanometer-based scanning and allow for an option to add resonant scanning. I.The system must be compatible with ScanImage control software to use with existing data sets. Time stamp transitor-transitor logic (TTL) signals should be available to temporally align imaging data, electrophysiology data and visual stimulation. J.The system must be capable of automatically collecting and storing XYZ stacks and must include the option to be controlled by external triggers or deliver a trigger to synchronize with other experimental electronics. K.The system must include software and hardware required for system calibration and troubleshooting, as well as a user manual. Anticipated period of performance: Delivery shall be required within six months after contractor receipt of order. Capability statement /information sought. Contractors that believe they possess the ability to provide the required equipment should submit documentation of their ability to meet each of the project requirements to the Contract Specialist. Responses shall include a capabilities statement and the following information: •information regarding respondents' commercially available equipment and technology related to this requirement; •respondents' opinions about the difficulty and/or feasibility of the potential requirement or proposed acquisition, possible solutions and approaches that may currently exist in the marketplace, and information regarding innovative ideas or concepts; The respondent must also provide their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2" x 11" paper size, with 1" top, bottom, left and right margins, and with single or double spacing. The information submitted must be must be in an outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. A cover page and an executive summary may be included but is not required. The response is limited to five (5) page limit. The 5-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents' technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer. Facsimile responses are NOT accepted. The response must be submitted to Ms. Lauren Phelps at e-mail address lauren.phelps@nih.gov. The response must be received on or before the closing date and time of this announcement. Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)."
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-2/HHS-NIH-NIDA-SSSA-SBSS-17-319/listing.html)
- Place of Performance
- Address: Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN04472894-W 20170416/170414234159-ec77e93481f37acc501e4acf797bb9b5 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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