SOURCES SOUGHT
66 -- Digital PCR
- Notice Date
- 6/5/2017
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Commerce, National Institute of Standards and Technology (NIST), Acquisition Management Division, 100 Bureau Drive, Building 301, Room B130, Gaithersburg, Maryland, 20899-1410, United States
- ZIP Code
- 20899-1410
- Solicitation Number
- NB644020-17-02966
- Archive Date
- 6/27/2017
- Point of Contact
- Latish Walker, Phone: 3019750474
- E-Mail Address
-
latish.walker@nist.gov
(latish.walker@nist.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a SOURCES SOUGHT NOTICE for market research purposes. THIS IS NOT A REQUEST FOR PROPOSALS. INFORMATION RECEIVED WILL BE USED FOR MARKET RESEARCH PURPOSES ONLY. After the results of this market research are obtained and analyzed, NIST may conduct a competitive procurement and subsequently award a contract. The National Institute of Standards and Technology (NIST), Biosystems and Biomaterials Division develops and applies standards and methods for all analytical and bio-analytical measurements to ensure bioassay performance for identification and quantitation of molecular and biological species. A digital PCR Instrument is used to determine the accurate and reproducible concentration of DNA samples. NIST requires a Digital PCR Instrument for use in certifying the concentration of Standard Reference Materials (SRM) that are produced in the Biosystems and Biomaterials Division. The instrument is required to produce high levels of data quality for the accurate measurement of the samples. In addition to high accuracy and reproducibility (precision) the instrument must also be able to measure large number of samples in a reasonable time (sample throughput) and at a reasonable cost per sample. A Droplet Digital PCR instrument works on the principle that single DNA molecules can be partitioned into droplet (compartments) and then PCR (polymerase chain reaction) is applied to amplify the single DNA molecules to detectable amounts. The PCR step can be done on a separate device (a thermocycler, many are already available at NIST) or part of the instrument. Each droplet that contains a single DNA molecule that is amplified by the PCR reaction and then detected by using a fluorescent dye molecule. The droplets that contain the amplified DNA are termed a positives and droplets that do not contain a DNA molecule are termed negatives. The fraction of negative droplets and the size of the droplets is used to calculate the concentration of DNA in the original sample. The instrument must have provisions for multiple samples, dispersion of the samples into the droplets and detection of the positives by detection of fluorescent dyes and software for data manipulation for calculation of the concentration values. NIST is aware of one instrument, the Bio Rad Laboratories model QX200 Droplet Digital PCR, which meets NIST's requirements. In the interest of promoting competition where possible, NIST is seeking information on third party resellers of the Bio Rad Laboratories model QX200 Droplet Digital PCR, as well as distributors of any other models/brands which are technically equivalent (i.e., meet the specifications detailed below). This information will be used to determine the best procurement strategy. Interested contractors shall supply the following information: 1. For products equal in function and performance to the Bio Rad Laboratories model QX200 Droplet Digital PCR, the contractor shall submit product documentation clearly showing how their product is equivalent in performance and function to the specified equipment as well as how their equipment can be installed. Specifications: • The droplet generator, the droplet reader, and the data analysis module must fit on a laboratory bench top size (each unit up to 48 inches in depth, 48 inches in width and 48 inches in height) • The droplet generator must capable of generating at least 20,000 droplets using a sample size of 20 microliters, allowing for detection of samples that differ in concentration up to 5 orders of magnitude • The instrument must be capable of detection of at least 2 fluorescent channels for at least 2 target sequences using common fluorescent dyes such as FAM, EvaGreen, HEX and VIC • The droplet reader capacity must have capability of automated reading for as few as 1 sample and up to 96 samples • The 96 samples must be analyzed by the droplet reader in less than 4 hours • The sample to sample reproducibility shall be within 10% of the calculated concentration values (precision). • The instrument shall include a data analysis module that is capable of collecting, storing and processing the data for at least 96 samples. • The instrument shall include software that can calculate the concentration of the DNA in each droplet for at least two fluorescent dyes by fitting the fraction of negative droplet data to a Poisson calculation to determine the copy numbers of the DNA targets • The software shall be able to display the fluorescent dye intensity for at least two dyes for each droplet. • The software shall be able to allow the adjustment of the threshold settings for calling positive droplets in individual wells or up to 96 samples. • The system shall include an operations and maintenance manual covering proper operation, routine maintenance, and troubleshooting for the instrument and controlling software 2. Resellers shall submit information showing that they are authorized to resell the specified equipment and that all manufacturer warranties and titles can transfer to NIST once equipment is delivered. Interested vendors shall submit their response via email to latish.walker@nist.gov no later than 11:00am on 12 June 2017. NIST's email system limits the total size of attachments to not more than 10Mb. Any single email exceeding that may be rejected by the NIST email server.
- Web Link
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FBO.gov Permalink
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- Record
- SN04532820-W 20170607/170605235226-996d77c6ee9a2f528a7ed1fdbc3a72c1 (fbodaily.com)
- Source
-
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