SOURCES SOUGHT
66 -- Fragment Analyzer
- Notice Date
- 7/7/2017
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- HHS-NIH-NIDA-SSSA-SBSS-17-535
- Archive Date
- 8/1/2017
- Point of Contact
- Sneha V Patel, Phone: 3014802443
- E-Mail Address
-
sneha.patel@nih.gov
(sneha.patel@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Solicitation Number: HHS-NIH-NIDA-SSSA-17-535 Title: Fragment Analyzer Classification Code: 6640 NAICS Code: 334516 Description: This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Background: The NIDA Genetic Engineering and Viral Vector Core (GEVVC) serves NIDA-IRP researchers by facilitating studies of brain function under physiological and pathological conditions through developing and producing genetic tools capable of modulating and monitoring the molecules, cells and circuits in the nervous system. The NIDA GEVVC offers the following services: (1) GEVVC services that are tailored to the needs of individual IRP researchers. Services include plasmid construction, packaging of custom Adeno-associated virus (AAV) and Lentivirus (LV) vectors, genome editing consultation for the design of CRISPR reagents for targeted mutagenesis or knock-in transgenes, development of genotyping assays, and BAC recombineering; and (2) training and access to equipment necessary for molecular biology techniques, i.e. for the construction of DNA vectors, genotyping, quantification of gene expression by ddPCR and qRT-PCR, expression and purification of proteins in bacterial and mammalian cell culture. GEVVC can use the Fragment Analyzer to: (1) streamline CRISPR workflows with reliable reagent quality control and a simple and accurate screening process while simultaneously elucidating the zygosity of positive clones; (2) determine the amplicon size and concentration of PCR fragments; (3) assess the amount of supercoiled plasmid DNA in a sample; (4) reliably and accurately assess genomic DNA size, quantity and quality; (5) reliably and accurately assess messenger and small RNA size, quantity and quality; (6) quantify and qualify DNA fragments and smears through 50 kilobases; and (7) assess the size and quantity of cell-free DNA (cfDNA) which is found in serum, plasma and cerebral spinal fluid (CSF) and can be potentially used as biomarkers in various diseases states, including brain injury. Purpose and Objectives: The NIDA Genetic Engineering and Viral Vector Core GEVVC needs a Fragment Analyzer which is capable of high resolution separation and quantification of DNA and RNA molecules to automate and standardize the characterization of mixed populations of nucleic acid fragments. This equipment will allow GEVVC to efficiently and cost-effectively categorize and quantify a wide range of DNA samples such as plasmids for viral vector production and PCR products amplified from CRISPR-edited cell lines and animals. This machine will also be used for similar analyses of total RNA and small RNA harvested from animal tissue and cell lines. The high resolution data generated by this machine will help investigators to decide whether or not to invest further time and resources on particular batches of samples, and should result in a substantial reduction in the amount of wasted effort. Project requirements: Fragment Analyzer with the following characteristics: 1. Technology must be centered on highly sensitive fluorescence-based, parallel capillary electrophoresis. 2. Analyze samples in as little as 15 minutes 3. View separations as they are generated and change electrophoresis times during a run if desired. 4. Direct parallel injection and separation of 12, 48, or 96 samples at once allowing the instrument to adjust throughput to fit the lab's needs. 5. The capillary array is stored in the equipment eliminating daily handling of the array. 6. Must have detection limits as low as 5pg/uL for DNA fragments and smears as low as 50pg/uL. Directly inject amplicon or restriction enzyme digestion product. 7. Must resolve a wide range of DNA and RNA fragments. Achieve as low as 3 basepair (bp) resolution with high sizing accuracy 8. High and Standard quantitative kits must include, but not be limited to: large fragment analysis for DNA 50bp-20,000bp; and RNA analysis for small RNA, microRNA, total RNA and messenger RNA. 9. Load and run up to three plates, or up to 288 samples, without human intervention. 10. Reagents are room temperature stable and no daily array handling 11. Requires only a single dilution step into the sample plate or tube strips. 12. Software packages which includes, but is not limited to: sample flagging for rapid scoring; smear analysis proving concentration, molarity, size range, average size, and CV%; ability to apply criteria to specific samples or all samples simultaneously; annotation features and customizable screen projection; and sample overlay with the ability to combine data from as many as 24 different runs Anticipated period of performance: Delivery on or before September 1, 2017. Capability statement: Contractors that believe they possess the ability to provide the required services should submit documentation of their ability to meet each of the project requirements to the Contract Specialist. The capability statement should include 1) the total number of employees, 2) documentation of ability to provide the required support, 3) any contractor GSA Schedule contracts by which all of the requirements may be met, if applicable, and 4) any other information considered relevant to this program. Contractors must also provide their Company Name, DUNS number, Physical Address, and Point of Contact Information. Interested organizations are required to identify their type of business, applicable North American Industry Classification System Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number. All capability statements sent in response to this Sources Sought Notice must be submitted to Sneha Patel, Contract Specialist, via email at sneha.patel@nih.gov before the closing date and time of this announcement. All responses must be received by the specified due date and time in order to be considered. Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)."
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