SOURCES SOUGHT
66 -- Procurement of All-In-One Fluorescence Microscope
- Notice Date
- 8/25/2017
- Notice Type
- Sources Sought
- NAICS
- 333314
— Optical Instrument and Lens Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- HHS-NIH-NIDA-SSSA-SS-17-764
- Archive Date
- 9/14/2017
- Point of Contact
- Danielle R. Brown, Phone: 301 480 2385
- E-Mail Address
-
danielle.brown2@nih.gov
(danielle.brown2@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Federal Business Opportunities (FBO) SOURCES SOUGHT NOTICE 1.Solicitation Number: HHS-NIH-NIDA-SSSA-SS-17-764 2.Title: Procurement of All-In-One Fluorescence Microscope 3.NAICS Code: 333314 - Optical Instrument and Lens Manufacturing 4.Size Standard: 500 Employees 5.Description: This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. 1.Background: The All-in-one microscope will allow complete imaging of tissue microarrays mounted on slides, multi-well experiments assessing the role of transcription factors in cell differentiation and aging rates of human iPS cells. The instrument will allow live cell imaging as well. The potential NIA applications of this microscope include, but are not limited to: •Ongoing studies of senescence (Gorospe lab, LGG) and aging in mice (SLAM, de Cabo lab, TGB) which can be facilitated by the user-friendly, high throughput, semi-automated imaging of tissue microarrays. •Single cell DNA damage assays (Comet assays) can be more efficiently imaged (Beerman lab, TGB). •Mitochondrial volume reconstructions can be rendered for whole organisms, tissue culture or isolated mitochondria, complementing the use of Seahorse system for measuring mitochondrial ROS and ATP generation.(Tyler Demarest, Bohr lab, LMG). For example, cultures analyzed in Seahorse plates can be re-imaged with the Keyence microscope system. •Wound invasion assays of NF-kB reporter expressing fibroblasts can be assessed on this setup as well. (Erik Martin, Sung lab, LMBI) Purpose and Objectives: The purpose of this acquisition is to procure a small stand-alone microscope with its own enclosed dark room with modular slide holders compatible with tissue culture flasks and plates, multi-well plates, coverslips and slides. The microscope must have bright field, fluorescence, and phase-contrast imaging with the capability for image stitching, Z-stack image capturing, and coordinate-specific condition setting. Project requirements: See Purchase Description Below. Quantity: One (1) Delivery Date: Within 30 days after receipt of order (ARO) Capability statement /information sought. Please provide a capability statement, this includes: •Provide evidence the respondent has performed services. •Provide evidence of similar previous contracts of research of the same magnitude. •Information regarding (a) staff expertise, including their availability, experience, and formal and other training; (b) current in-house capability and capacity to perform the work; (c) prior completed project of similar nature; (d) corporate experience and management capability; and (e) examples of prior completed Government contracts, references, and other related information. Please provide your DUNS number, organization name, address point of contact, and size and type of business (e.g., 8(a), HubZone, etc.,) pursuant to the applicable NAICS code and any other information that may be helpful. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2" x 11" paper size, with 1" top, bottom, left and right margins, and with single or double spacing. The information submitted must be must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. The response is limited to one to two (1-2) page limit. The 1-2 page(s) do not include the cover page, executive summary, or references, if requested. All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer. Facsimile responses are NOT accepted. The response must be submitted to Danielle R. Brown, Contract Specialist, at Danielle.brown2@nih.gov. The response must be received on or before Wednesday, August 30, 2017 at 12:00PM, Eastern Standard Time. "Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)." PURCHASE DESCRIPTION 1.Statement of Need and Purpose: The purpose of this acquisition is to procure a small stand-alone microscope with its own enclosed dark room with modular slide holders compatible with tissue culture flasks and plates, multi-well plates, coverslips and slides. The microscope must have bright field, fluorescence, and phase-contrast imaging with the capability for image stitching, Z-stack image capturing, and coordinate-specific condition setting. 2.Background Information and Objective: The All-in-one microscope will allow complete imaging of tissue microarrays mounted on slides, multi-well experiments assessing the role of transcription factors in cell differentiation and aging rates of human iPS cells. The instrument will allow live cell imaging as well. The potential NIA applications of this microscope include, but are not limited to: •Ongoing studies of senescence (Gorospe lab, LGG) and aging in mice (SLAM, de Cabo lab, TGB) which can be facilitated by the user-friendly, high throughput, semi-automated imaging of tissue microarrays. •Single cell DNA damage assays (Comet assays) can be more efficiently imaged (Beerman lab, TGB). •Mitochondrial volume reconstructions can be rendered for whole organisms, tissue culture or isolated mitochondria, complementing the use of Seahorse system for measuring mitochondrial ROS and ATP generation.(Tyler Demarest, Bohr lab, LMG). For example, cultures analyzed in Seahorse plates can be re-imaged with the Keyence microscope system. •Wound invasion assays of NF-kB reporter expressing fibroblasts can be assessed on this setup as well. (Erik Martin, Sung lab, LMBI) 3.Generic Name of Product: All-In-One fluorescence microscope 4.Purchase Description: 1BZ-X710 BZX Fluorescence Microscope 2BZ-X700E BZX Fluorescence Microscope 3972318 Win10 PC for BZ-X Dell T3420 4972072B BZX Accessory 23'' Monitor 5 BZ TRAININGBZ Training 6 BZ-H3XDBZX Software 7 BZ-H3XTBZX Software BZX Time Lapse 8 BZ-H3AEBZX Software BZX Analyzer Software 9 OP-87762BZX Accessory BZX DAPI Filter 10 OP-87763BZX Accessory BZX GFP Filter 11 OP-87765BZX Accessory BZX Texas Red Filter 12 OP-87766BZX Accessory BZX Cy5 Filter 13 972081CFI PLAN FLUOR DL 4X LENS 14 971936CFI PLAN FLUOR DL 10X LENS 15 971962Nikon Lens (20x ELWD Phase) for BZ S PI FI ELWD ADM 20xC 16 971963Nikon Lens (40x ELWD Phase) for BZ S PL FL ELWD ADM 40xC 17 972082INUG2-KIW STAGE TOP INCUBATOR 5.Salient characteristics: Microscope unitBasic optical systemInverted fluorescence phase contrast microscope Objective lensesNikon CFI 60 Series infinite optical system Observation modesBright field, Fluorescence (wide-field/sectioning), Phase contrast (PhL, Ph1, Ph2), Oblique illumination Objective lens switchingSix-mount electronic revolver Image-formation optical systemFixed image-forming lens, electronic LC filter insertion / removal unit Electronic XY stage100×64 mm stroke, 1 um pitch min. Electronic Z stage8 mm stroke, 0.1 um pitch min. Electronic filter turretUp to four filters can be mounted. Automatic position recognition and automatic excitation shutdown during filter replacement Fluorescent incident illuminationOptical sectioning system Fluorescence dimming mechanismElectronic dimming (0.3% 5% 10% 20% 40% 100%) Transmitted illumination optical systemOperating distance : 30 mm, Pop-up mechanism (with automatic lamp off function) Transmitted illumination mechanismElectronic switching, Electronic bright-field aperture (0% 20% 40% 60% 80% 100%) / Phase-contrast slit (Ph1 / Ph2) Transmitted light sourceLED 3.7W, 50,000 hours (average) Fluorescent incident light sourceMetal halide lamp 80W, 2,000 hours (average) Specimen enclosureThe stage is fully contained in a built-in darkroom. Camera unitImage pick-up element2 / 3 inch, 2.83 million pixel monochrome CCD (colourised with LC filter) CCD cooling mechanismPeltier cooling : 5°C 41°F (at ambient temperature of -25°C -13°F) Output signal, gradationsMonochrome 14bit / 8bit, 8 bit for RGB each Frame rate15 fps for monochrome recording (up to 95 fps with binning), 8.5 fps for color recording BinningOn-chip binning (2×2、3×3、4×4、8×8、12×12) Number of pixels in recorded image4,080 × 3,060 max (12.5 million pixels, high-quality interpolation) Video capture8-bit monochrome: 15 fps for 1,280 x 960 With binning: 28 fps for 960 x 720, 40 fps for 640 x 480, 50 fps for 480 x 360, 75 fps for 240 x 180, 95 fps for 160 x 120 Color: 8.5 fps for 1,280 x 960 Electronic shutterAuto : 1 / 7500 to 60 sec. (77 increments) Auto Exposure methodArbitrary area, average / peak Gain0dB、+6dB、+12dB、+18dB White balanceMANUAL, PUSH-SET Black balance Observation softwareMulti-color image capturing, Auto focus, Quick full-focusing, Scale display, Electronic revolver control, Electronic stage control ControllerApplicable OSWindows7 Professional 64bit SP1*1 PC interfaceUSB2.0 Ambient temperature+15 to +35°C 59 to 95°F Relative humidity35 to 80% RH (No condensation) DimensionsHead : 517 (H) × 340 (W) × 496 (D) mm*2 / Controller : 227.5 (H) × 125 (W) × 403 (D) mm WeightHead : 33 kg / Controller : 4.8 kg Power voltage100 to 240 VAC ±10% 50 / 60 Hz Power consumption250VA Overvoltage categoryII Pollution degree2 Software (optional)Sectioning ModuleOptical sectioning image mode Advanced Observation ModuleTypeNavigation, Image stitching, Z-stack image capturing, Coordinate-specific condition setting Navigation ModuleNavigation Image stitching ModuleImage stitching, Z-stack image capturing, Coordinate-specific condition setting Time-lapse ModuleTime-lapse imaging, Video capturing Advanced Analysis SoftwareImage stitching, Haze reduction, Full focus Measurement ModuleXY measurement, Area measurement, Brightness measurement (line profile, histogram) 3D ModuleReal-time 3D analysis, XYZ slicing, Maximum projection Hybrid Cell Count ModuleCell count (phase contrast, bright field, fluorescence images), Mask cell count Macro Cell Count ModuleMacro Cell Count (batch analysis of multiple images) 6.Quantity: One (1) 7.Delivery Date: Within 30 Days after receipt of order (ARO)
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/HHS-NIH-NIDA-SSSA-SS-17-764/listing.html)
- Place of Performance
- Address: 251 Bayview Boulevard, Baltimore, Maryland, 21224, United States
- Zip Code: 21224
- Zip Code: 21224
- Record
- SN04648547-W 20170827/170825232501-f2eb956339128e2321e0dce4728e4433 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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