SOLICITATION NOTICE
66 -- Inverted Total Internal Reflection (TIRF) Microscope System for Single Molecule Studies at NINDS - FAR 52.212-5 Applicable Clauses - FAR 52.212-3 Reps and Certs
- Notice Date
- 6/7/2018
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- NIH-NIDA-CSS-18-4949480
- Archive Date
- 6/20/2018
- Point of Contact
- Jason Williams, Phone: 3014965985, Nancy Lamon-Kritikos, Phone: 301-480-2447
- E-Mail Address
-
Jason.Williams1@nih.hhs.gov, nancy.lamon-kritikos@nih.gov
(Jason.Williams1@nih.hhs.gov, nancy.lamon-kritikos@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- FAR 52.212-3 Offeror Representation and Certifications -- Commercial Items FAR 52.212-5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders -- Commercial Items -- Applicable Clauses Title: Inverted Total Internal Reflection (TIRF) Microscope System for Single Molecule Studies at NINDS This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The solicitation number is NIH-NIDA-CSS-18-4949480 and the solicitation is issued as a request for quotation (RFQ). This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures; FAR Subpart 13.5-Simplified Procedures for Certain Commercial Items; and FAR Part 12-Acquisition of Commercial Items, and is expected to exceed the simplified acquisition threshold. The associated NAICS code is 334516, "Analytical Laboratory Instrument Manufacturing" and the small business size standard is 1,000 or fewer employees. This acquisition is unrestricted, with no small business restrictions. Both large and small business offers shall be considered for award. Contract Type A firm-fixed price type contract is contemplated for this requirement. Statement of Need and Purpose : An Inverted Total Internal Reflection (TIRF) Microscope is needed to perform single molecule imaging in support of research to be conducted by the Molecular Neurophysiology Section (MNS) of the National Institute of Neurological Disorders and Stroke (NINDS). Background Information and Objective: The National Institute of Neurological Disorders and Stroke (NINDS)-Molecular Neurophysiology Section (MNS) studies the conformational dynamics of proteins, focusing on the large-conductance voltage- and calcium-dependent potassium (BK) channels, Na+/K+ ATPase and Adenosine Deaminase Acting on RNA (ADAR). The first two are membrane proteins and the third one is a cytosolic protein that modifies mRNA at specific sites resulting in many instances with a change in codon that ultimately produce a protein with altered functions. Many substrates of ADAR2 are membrane proteins, including those of our immediate interest. Recently, MNS has begun applying fluorescence approaches to understand the dynamics of membrane proteins by measuring simultaneously ionic currents and fluorescence signals from a population of proteins. For many questions, the present approach is informative; yet, there is an unparalleled understanding of a biological system once you study it at the single molecule level. MNS is proposing to set up a microscope to perform single molecule Fluorescence Resonance Energy Transfer (smFRET). One example of the power of this approach is the possibility to track conformational changes of individual molecules in real time. This information allows the characterization of the time that a molecule dwells between two or more states, which provides a direct determination of the reaction rates of specific transitions. Typically, Cy3 (donor) and Cy5 (acceptor) fluorophores are used. These molecules can be incorporated into proteins by two ways: 1) cysteine substitution followed by chemical modification with cysteine-reactive fluorescence probes or 2) site-directed incorporation of unnatural fluorescent amino acids. The Imaging Probe Development Center from the National Institutes of Health has already synthesized for MNS three unnatural fluorescent amino acids: BODIPY, Cy3 and Cy5, all with short linkers between the fluorophore and the alpha carbon. From the biological perspective, we are ready to begin experiments at the single molecule level. Generic Name of Product: Inverted Total Internal Reflection (TIRF) Microscope System for Single Molecule Studies Sample Product: Nikon Ti2-E Inverted TIRF Microscope for Dual Color Single Molecule Imaging Salient characteristics The NINDS-MNS requires (1) Inverted Total Internal Reflection (TIRF) microscope system (composed of microscope and component parts) for single molecule studies system that meets all the following salient characteristics: 1.System must have a 25mm field of view with transmitted and fluorescence illumination engineered specifically for flatness across the 25mm field. 2.System must have a motorized encoded XY stage, high precision Z motor to achieve real-time compensation of focus drift, which is important for TIRF and single molecule imaging. 3.System must have a TIRF illuminator actuated by micrometers that afford extremely fine control of beam angle; this is advantageous for optimizing signal to noise during single molecule imaging. 4.System must have a 100X Apo TIRF 1.49 NA oil lens, apochromatically corrected across the visible range, including a correction collar for point spread function optimization. 5.System must include a laser safe camera port with an integrated Bertrand lens to safely view the laser beam spot during angle changes in TIRF imaging. 6.System must be flexible to expand from a single TIRF input to include multiple additional TIRF illuminators onto a single microscope in flexible wavelength and motorization combinations. 7.System must be driven by software that support for camera-timed hardware triggering, dual triple and quad camera synchronization, custom acquisition scripting and live ratio/FRET calculations. 8.System should be directly triggered by a camera timing signal. 9.System should be compatible for upgrades, including field scanning and laser scanning confocal, STORM and SIM superresolution, as well as FLIM and FCS devices. 10.A focus system with offset lens is needed to correct Z drift in real time during imaging. 11.System has to have two cameras with single photon counting mode; amplification of signal to increase the signal to noise ratio Quantity : One system consisting of various components and software as described in the salient characteristics above. Please submit price quote itemized by system components. Contract Type : A firm fixed price type contract is anticipated for this acquisition Delivery Date : 30 days after receipt of order Delivery Location: National Institutes of Health Porter Neuroscience Research Center Bldg 35 35 Convent Drive Bethesda, MD 20892 Warranty: Industry standard The provision at FAR clause 52.212-1, Instructions to Offerors - Commercial Items, applies to this acquisition. The provision at FAR clause 52.212-2, Evaluation - Commercial Items, applies to this acquisition. (a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to this solicitation will be most advantageous to the Government on the basis of lowest price, technically acceptable. The following factors shall be used to evaluate offers: In addition to price, the Government will award a contract on the basis of technical acceptability in accordance with the Salient Characteristics section of the Purchase Description, the vendor's ability to meet the required delivery schedule contained in the Delivery Date section, and the required warranty as indicated in the Warranty section of the Purchase Description contained in this combined synopsis/solicitation. Past performance shall also be considered. (b) A written notice of award or acceptance of an offer, mailed or otherwise furnished to the successful offeror within the time for acceptance specified in the offer, shall result in a binding contract without further action by either party. Before the offer's specified expiration time, the Government may accept an offer (or part of an offer), whether or not there are negotiations after its receipt, unless a written notice of withdrawal is received before award. The Offerors to include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications-Commercial Items, with its offer. Please indicate country of manufacture. The FAR clause at 52.212-4, Contract Terms and Conditions - Commercial Items, applies to this acquisition. FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items, applies to this acquisition. See attached document, "FAR 52.212-5 Applicable Clauses", for clauses applicable to this acquisition. The following additional contract requirement(s) or terms and conditions as determined by the contracting officer are necessary for this acquisition and consistent with customary commercial practices: There are no additional contract requirement(s) or terms and conditions applicable to this acquisition. The Defense Priorities and Allocations System (DPAS) are not applicable to this requirement. Responses to this solicitation must include sufficient information to establish the interested parties' bona-fide capabilities of providing the product or service. The price quote shall include: unit price, list price, shipping and handling costs, delivery days after contract award, delivery terms, prompt payment discount terms, F.O.B. Point (Destination or Origin), product or catalog number(s); product description; and any other information or factors that may be considered in the award decision. Such factors may include: past performance; special features required for effective program performance; trade-in considerations; probable life of the item selected as compared with that of a comparable item; warranty considerations; maintenance availability; and environmental and energy efficiency considerations. In addition, the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." All responses must be received by 5 PM EST June 15, 2018 and reference number NIH-NIDA-CSS-18-4949480. Responses shall be submitted electronically to Jason Williams, Contracting Officer at willjas@nida.nih.gov Fax responses will not be accepted. For any questions or further information regarding this solicitation, please contact Jason Willliams, Contracting Officer, NINDS Contracts Management Branch, NIDA, NIH at PH: 301-496-5985; E-mail: willjas@nida.nih.gov
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/NIH-NIDA-CSS-18-4949480/listing.html)
- Place of Performance
- Address: Contractor's facility, United States
- Record
- SN04947774-W 20180609/180607230941-94ed0234499cf5c80102552b1054192b (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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