SPECIAL NOTICE
66 -- Notice of Intent Sole Source Nikon Eclipse Ti2-E with Perfect Focus Microscope System for NINDS
- Notice Date
- 6/26/2018
- Notice Type
- Special Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- NIH-NINDS-NOI-18-4949992
- Archive Date
- 7/12/2018
- Point of Contact
- Nancy Lamon-Kritikos, Phone: 301-480-2447, Jason Williams, Phone: 3014965985
- E-Mail Address
-
nancy.lamon-kritikos@nih.gov, Jason.Williams1@nih.hhs.gov
(nancy.lamon-kritikos@nih.gov, Jason.Williams1@nih.hhs.gov)
- Small Business Set-Aside
- N/A
- Description
- THIS IS A PRE-SOLICITATION NOTICE OF INTENT TO AWARD ON A SOLE SOURCE BASIS. THIS IS NOT A REQUEST FOR QUOTE/PROPOSALS. The NINDS Contracts Management Section of the National Institute on Drug Abuse (NIDA), intends to solicit on a sole-source basis by a request for quotation to Nikon Instruments, Inc. on or around July 13, 2018 with an award issued on or around July 27, 2018. The purpose of the resultant contract will be to provide the National Institute of Neurological Disorders and Stroke (NINDS) with a Nikon Ti2-E with Perfect Focus Microscope System in order to conduct genetic screens to help identify interactors and partners in different biological pathways. This type of novel screen requires the use of a high-resolution, high-speed, movement-sensitive microscope. Additionally, it requires an open-source infrastructure that will allow NINDS to use its customized interface. The NINDS has developed custom machine learning software for extant Nikon equipment and would be cost and time prohibitive to re-produce for non-Nikon equipment. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE and SET ASIDE STATUS The intended procurement is classified under NAICS code 334516 with a Size Standard of 1,000 employees or fewer. This acquisition is NOT set aside for small businesses, but rather is a sole-source acquisition to Nikon Instruments, Inc. REGULATORY AUTHORITY This acquisition is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 6.302-1, Only one responsible source and no other supplies or services will satisfy agency requirements. BACKGROUND, PURPOSE AND OBJECTIVES OF REQUIREMENT Neurodegenerative disorders are broadly defined as a heterogeneous group of diseases all presenting with gradual, progressive loss of neuronal structure and function. One of the common pathophysiological features of many neurodegenerative disorders is the presence of dysfunctional mitochondria. Most notably, mitochondrial dysfunction, along with misfolded protein accumulation, are hallmarks of some of the most prevalent neurodegenerative disorders - Huntington's Disease, Parkinson's Disease, Alzheimer's Disease, and Amyotrophic Lateral Sclerosis - and many other less common neurodegenerative diseases. In each of these conditions, multiple mitochondrial functions are impaired, ranging from mitochondrial morphology and aggregation to ATP formation. While all of these conditions exhibit loss of mitochondrial function, the specific mechanisms behind the loss of function are still being elucidated. Of the many neurodegenerative disorders with links to mitochondrial dysfunction, Parkinson's Disease is the most well-studied. In cell biology, the identification of new genetically encoded pathways is of essential for scientific advancements and drug development. Over the years, scientists have relied on genetic screens to help identify interactors and partners in different pathways. Historically, these genetic screens were performed in yeast; but in order to develop therapeutic treatments for humans, this work is best performed in human cells. Traditionally, RNAi-based screens have been used to elucidate pathways in human cells. In order to help elucidate the contribution of mitochondria to Parkinson's Disease progression, we have used functional genomic screens to identify novel regulators in the past. However, this type of approach has limitations, namely, lack of specificity leading to numerous false positive hits. Another limitation of functional genomic screens is that they rely on connecting genetic perturbations to a phenotype on a subcellular level. In order to establish this connection, an arrayed screen is often used, where hundreds of thousands of samples are perturbed on a mass scale; this creates a bottleneck of data analysis. Additionally, the reproducibility is limited. Therefore, we have developed a novel technique that uses machine learning to detect pre-determined phenotypes in a pooled screen. With this approach, we are able to bypass the limitations of an arrayed screen because the sampling number is smaller. Additionally, by using a revolutionary genetic engineering technology - CRISPR/Cas9- we have a screening method more specific than RNAi. Importantly, this pooled screen is more financially sustainable than an arrayed screen. At the moment, our approach is limited by the equipment that we use. The microscope that we currently use is not meeting the needs of the project effectively. This type of novel screen requires the use of a high-resolution, high-speed, movement-sensitive microscope. In addition, it requires an open-source infrastructure that will allow us to use our customized interface. Currently, there is no microscope on the NINDS premises that can perform all of the necessary functions. This type of genetic screen can only be performed on a microscope that has both high-resolution and high-speed in a wide field of view. These parameters will allow us to not only sample more efficiently but also more quickly. By using the unique open-source NIKON Elements module, we will be able to perform on-the-fly image analysis allowing us to collect pre-defined phenotypes. Ultimately, we will be able to interface all parameters of the microscope while simultaneously incorporating our customized algorithms. Neurodegenerative disorders are a devastating class of diseases with few therapeutic options and largely unresolved mechanisms of disease progression. This screen will aid us in revealing new aspects of cellular pathways that could ultimately be used in developing therapeutic treatments. CONTRACTING WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME) DETERMINATION The Spinning Disk Confocal Microscope must interface with internally-developed custom machine learing software that was developed for existing Nikon microscopes in the lab. This custom machine learning software is capable of interfacing with a confocal microscope to capture images, laser stimulation, and analysis on-the-fly. Using support vector machine-based learning, the NINDS developed algorithm uses images captured and processed by the microscope to identify pre-determined phenotypes and then subsequently feeds this information back into the microscope system to isolate cells of interest. Based on this co-dependency, the NINDS-developed algorithm must interface perfectly with the imaging software. The NINDS custom module was integrated within the Nikon software environment during the development of the algorithm. The synchronization of the algorithm with image acquisition steps was a complex process which required immense technical support. This internally-developed software provides users with all the tools to anlyze complex images. Futhermore, all of these capabilities are available to use during image acquisition, rather than needing to be applied post-acquisition, thereby creating a seamless platform. In order to create this platform with any equipment other than the Nikon Brand Name equipment would require immense effort from hardware and software engineers causing unacceptable delays and duplication of costs toward planned research efforts. In accordance with FAR part 10, extensive market research was conducted to reach this determination. Specifically, a Sources Sought notice was posted to FedBizOpps referencing the above detailed requirements and no responses were received. Additionally, contacts with knowledgeable individuals both in industry and Government, as well as reviews of available product literature, revealed no other sources. A review of the GSA Advantage, Dynamic Small Business Search, and previous government acquisitions returned no results that meet all of the requirements. Finally, Nikon Instruments verified that there were no authorized dealers/distributors for this particular equipment. Therefore, only Nikon Instruments, Inc. is found to be capable of meeting the needs of this requirement. The intended source is: Nikon Instruments, Inc. 1300 Walt Whitman Rd, Fl 2 Melville, NY 11747-3001 Trade Name/Model/Part Number: (1) Trade name: Nikon Eclipse Ti2-E Spinning Disk Confocal Microscope with Perfect Focus (2) Product's brand name: Nikon Eclipse Ti2-E Spinning Disk Confocal Microscope with Perfect Focus (3) Manufacturer's name: Nikon Instruments (4) Model and part number: Nikon P/N Qty Descripition MEA54000 1Ti2-E Inverted Motorized Microscope Stand MEF550371Ti2-E Controller MXA221491S-TI2-EXT External Trigger Cable MEB558301Ti2-T-BS Binocular Body Base Unit (Simple) MEB525201TC-T-ER Ergo Binocular Tube MAK101102CFI 10X EYEPIECE F.N. 22MM-NC MEP593941Ti2-ND-P Perfect Focus System 4 MEE599201Ti2-D-PD Diascopic Illumination Pillar MEE557001Ti2-D-LHLED Transmitted Light LED Lamphouse MEV500101Ti2-D-SF Diacopic Illumination Slider MBN117101Daylight NCB11 Filter, 45mm MBN112001Filter 45mm, GIF Green Interference (546/60nm) MBN218161Filter 45mm, ND16A Multi-Coated Neutral Density MBN218081Filter 45mm, ND8A Multi-Coated Neutral Density MEL510051TC-C-TC Condenser Turret, Seven Position MEL562001TC-C-LWD Lens Unit for System Condenser Turret MEV510301Ti2-F-FLT-E Motorized Epi Fluorescence Turret MXA221471S-TI2-DCL Daisy Cable Long MXA221461S-TI2-DCS Daisy Cable Short MEV500201TI2-F-FSC Circle Field Stop Slider MXA221521Ti2-D-LSK Laser Safety kit MXA221581S-Ti2I-LU Interlock Cable MEE548401TI2-LA-BM-E Motorized Main Branch MEE547301TI2-LA-FLL Epi Fluorescence Module Large Field 770600851SOLA SE II Light Engine, Solid State While-Light 770600212Liquid Light Guide for Lumencor, 3mm diam 963691C-FLL Large Field of View DAPI SOLA Filter Set 963721C-FLL Large Field of View GFP/FITC/Cy2 Filter Set 963741C-FLL Large Field of View DSRed/TRITC/Cy3 Filter 963761C-FLL Large Field of View Cy5 Filter Set 970501USB 2.0 A-B 15ft Cable 790351Power Cord 120V, Lead Free 999281CSU-W1 50um pinhole disk array 770145672ET455/50m, DAPI-ET Emission Filter 770148032ET525/36m Emission Filter, max blocking 770744932ET605/52m Emission Filter 25mm 770743292ET705/72m Emission Filter, Far Red Emission 77074021269000M DAPI/FITC/TRITC TRPL BP FLTR 25MM 999311Ti sealed microscope adapter for CSUW-1 MQD420051C-DA C-Mount/Iso Adapter 1x 999331DM A561LP for CSUW-1 999361Full Mirror for CSUW-1 999351Dummy Glass for CSUW-1 770182911Photometrics Prime 95B Back-illum sCMOS camer MEE581001TI-LA-DMS DMD Module MEE548801TI2-LA-BS Sub Branch MEV530021TI-LA-LEDAD LED Adapter MQF520551AC Adapter 973371C-TIRF Filter Cube 405nm Reflection 770600791AURA Solid State Triggerable Illuminator 770600171DB 15HD to 7Xbnc cable for TTL control MEE580172TI-LA-Exchangable Mirror (100% Reflect) 911721405nm Reflect/430-800nm Transmit Mirror 790351Power Cord 120V, Lead Free 770380331P-736.ZRN2S PlNano Z K Plate Scanning System 770380241Microscope Universal Holder for Slides and Petri 770380071Well Plate Holder for Pl Nano Z 200um piezo stage 770380061Slide Holder for PI Nano Z 200um stage 770574371Custom Microscope Lexan Enclosure for CO2 770575001H2O1 Gas chamber for Physik Instr PI P-736 770573431Temperature control unit - Bold Line 770573331Touch Screen Controller 770575281Multi-Well Plate insert for H201 Incubation System 7705746311"x3" chamber slider holder - magnetic 770574611Lab-Tek II 1"x2" Chambered Cover Glass Holder 770574341Optional KOEHLER Lid 770573481CO2 Controller - Bold Line 770573421Air pump - Bold Line 770574361Vibration Free Humidifying Module 770115231Prior High Speed Linear Motor Stage for Nikon Ti 770115241Prior Proscan III LD controller with linear drives 770115451Joystick and Interactive Control Center MQS411001NIS-Elements: Hardware Module MRD001051CFI60 Plan Apochromat Lambda 10x Obj Lens MRD702001CFI60 Plan Aprochromat VC 20x Obj Lens MRD772001CFI60 Aprochromat Lambda S LWD 20x Water Im MRD774101CFI60 Apochromat Lambda S LWD 40x Water Im MRD006051CFI60 Plan Apochromat Lambda 60x Obj Lens MEV540061TI2-N-WID Water Immersion Dispenser 770197171Advanced Biosystem Workstation HP Z840 77019701227" LED Backlit IPS narrow bezel monitor 770132401NI PXI-1033 Controller; 5 Slot PXI Xchassis 770132411NI PXI 6723 DAQ 770132351BNC-2115 Shielded BNC connector block 770132261NI BNC-2110 Shielded BNC and terminal conn 770132272SHC68-68-EPM, 68-D-Type to 68 VHDCI Offset MQS340001NIS-Elements High Content Package for Multiwell MQS425601NIS-Elements: Module 6D Imaging MQS419601NIS-ELEMENTS HARDWARE MODULE MQS419301Device control for Sutter DG4/5, AOTF, Agilent, MQS412201NIS-Elements: Hardware Module Wavelength contr MQS419201Controls Shutter and Illumination EXFO and SOLA MQS427801Device Control for External Triggering th camera MQS413201NIS-Elements Hardware Module Motor Focus Cntrl 770197181Zenith Workstaton HP Z840 MQS315001NIS-Elements Adv Research Passive License MQS430401NIS-Elements High Content Upgrade Module 77049153136"x48" Vibration Isolation Table 770490131Set of 4 Casters for Vibration Isol Table 920841Large Nycle Dust Cover 14"x26.5"x32" MXA22168130cc Non-Fluorescing Immersion Oil F 770491071Subshelf for Vibration Isolation Table MED545501Ti2-FP Fixing Plate Salient characteristics: Nikon Instruments' newest inverted microscope, the Eclipse Ti2-E improves upon the market-leading performance of the Eclipse Ti with several key enhancements, including an industry-leading 25mm field of view, Perfect Focus 4 drift correction system, and several key improvements in optical and mechanical design. This particular instrument offers the largest available field of view on the market, increased motor speed and stability, as well as compatibility with various imaging modalities. System includes a CSU-W1 model spinning disk confocal scanner from Yokogawa. This system provides increased pinhole spacing and thus reduced spatial crosstalk between adjacent pinholes, a dual camera scanhead, and dual emission filters. Furthermore, this system comes with the powerful NIS-Elements software, providing a comprehensive suite of acquisition and analysis features. Importantly, this NIS-Elements software is fully compatible with custom acquisition scripting, allowing us to handle image processing and analysis using our custom-made program. Due to the features detailed above and many more, the Eclipse Ti2-E is the only microscope that could work for our unique needs. Delivery Date : 60-90 days after the order is received Delivery Location: National Institutes of Health Porter Neuroscience Research Buidling Bldg. 35, Rm. 2C917 35 Convent Drive Bethesda, MD 20892 Contract Type: A firm-fixed price type contract is planned for this acquisition consistent with commercial practices. Warranty : Industry standard CLOSING STATEMENT THIS NOTICE OF INTENT TO AWARD ON A SOLE-SOURCE BASIS IS NOT A REQUEST FOR COMPETITIVE PROPOSALS. However, interested parties may identify their interest and capability to respond to this notice. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. All responsible sources may submit a capability statement, proposal, or quotation which shall be considered by the agency. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov. All responses must provide evidence that they can provide equipment that meets all of the requirements in this announcement. All responses must be received by the closing date and time of this announcement and must reference the solicitation number, NIH-NINDS-NOI-18-4949992. Responses must be submitted electronically to Nancy Lamon-Kritikos, Section Head, NINDS Contracts Management Branch, NIDA, at nancy.lamon-kritikos@nih.gov. U.S. Mail and Fax responses will not be accepted.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/NIH-NINDS-NOI-18-4949992/listing.html)
- Place of Performance
- Address: Contractor's facility, United States
- Record
- SN04969833-W 20180628/180626231347-8a3e544847c1ded2e9656af7d6ade396 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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