SOURCES SOUGHT
66 -- Imaging Cytometer
- Notice Date
- 7/19/2018
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 6001 Executive Boulevard, Room 3165, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-(AG)-SBSS-18-355
- Archive Date
- 8/11/2018
- Point of Contact
- Andrea McGee, Phone: 301480-2449
- E-Mail Address
-
amcgee@nida.nih.gov
(amcgee@nida.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- SOURCES SOUGHT NOTICE 1.Solicitation Number: HHS-NIH-NIDA-(AG)-SBSS-18-355 2.Title: Imaging Cytometer 3.NAICS Code: 334516 Analytical Laboratory Instrument Manufacturing 4. Description: Lab Equipment This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only. Background: The assessment of cell viability and proliferation rate are standard cell culture techniques which are required for the thorough investigation of genotype and phenotype alterations in age-related research. Current technologies require the trypsinization and/or staining of cells with toxic chemicals, which perturbs their natural state and requires the use of a lot of materials (i.e. dishes, media, and drugs) because current techniques also consume an entire cell sample for one time-point. The imaging cytometer will allow us to non-invasively monitor the same cells over time, with different treatments, without having to plate multiple duplicates for time course experiments. This will provide more accurate data with less reagent and materials used in numerous cell culture formats. Moreover, we routinely assess mitochondrial function using the Seahorse XFe96 extracellular flux analyzer. This requires a normalization step to account for the number of cells, to ensure the results are due to mitochondrial functional differences rather than differences in cell number or protein content. Current normalization techniques require cell lysis and protein determination, which is time consuming with a large degree of inherent variability. This method also assumes that there are no differences in total cellular protein content between treatment groups, which may not be the case, especially in experiments where proteins are overexpressed or knocked down for experimental purposes. Using the imaging cytometer, we will be able to count the exact number of cells in each of the 96 wells before and after an experiment using the Seahorse. This will allow us to account for any loss of cells during the course of the experiment, as well as proper normalization per cell, without the need to make assumptions like the current protein content normalization approach. Overall, the imaging cytometer provides a novel, non-invasive tool to more accurately assess and quantify critical cell functions while saving time and money in wasted reagents. Purpose and Objectives: The purpose of this requirement is to purchase a Imaging Cytometer with 5 channels to enhance our studies on mitochondria and aging. Purchase Description: The Imaging Cytometer must be able to scan the entire well and all 96 wells in a plate and perform analysis at the same time in under 10 minutes. Must be able to read plates made for use in a Seahorse Analyzer to allow for normalization of Seahorse experiments. Must be able to image the entire well at one time. Must have flow-like gating capability enabling sub-population and cell cycle analysis Must be able to analyze in 5 channels (BF and 4FL). The machine and software should be ready to use with minimal training and have built in applications Anticipated delivery: 60 days after receipt of order Place of Performance: 251 Bayview Blvd Baltimore, MD 21224 Capability statement /information sought. Contractors that believe they possess the ability to provide the requirement should submit documentation of their ability to meet each of the project requirements to the Contract Specialist. The response should directly and specifically state in the, capabilities statement, which project requirements you can supply. Contractors must also provide their Company Name, DUNS Number, Physical Address, Point of Contact, and Size and Type of Business (e.g., 8(a), HubZone, etc.) pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2" x 11" paper size, with 1" top, bottom, left and right margins, and with single or double spacing. The information submitted must be must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. A cover page and an executive summary may be included but is not required. The response is limited to ten (10) page limit. The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents' technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. All responses (capability statements) sent in response to this Small Business Sources Sought Notice must be submitted electronically (via e-mail) to the Contract Specialist. Facsimile responses are NOT accepted. The response must be submitted to Andrea McGee, Contract Specialist at e-mail address amcgee@mail.nih.gov The response must be received on or before July 27, 2018 at 12:00PM, eastern time. "Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s)."
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-2/HHS-NIH-NIDA-(AG)-SBSS-18-355/listing.html)
- Place of Performance
- Address: 251 Bayview Boulevard, Baltimore, Maryland, 21224, United States
- Zip Code: 21224
- Zip Code: 21224
- Record
- SN04998209-W 20180721/180719230759-9f4834c68373cce83f5ed0cfc660287e (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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