SOLICITATION NOTICE
R -- HEK293 and HepG2 Knockout Cell Lines with GenCRISPR - Attachment
- Notice Date
- 9/9/2019
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541990
— All Other Professional, Scientific, and Technical Services
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211, MSC 9559, Bethesda, Maryland, 20892-9559, United States
- ZIP Code
- 20892-9559
- Solicitation Number
- 75N95019Q00345
- Archive Date
- 9/26/2019
- Point of Contact
- Hunter A Tjugum, Phone: 3018275304
- E-Mail Address
-
hunter.tjugum@nih.gov
(hunter.tjugum@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- invoice and payment provisions 52.204-24 (i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. (ii) The solicitation number is 75N95019Q00345 and the solicitation is issued as a Request for Quotation (RFQ) on a Full and Open competitive basis. This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures; FAR Subpart 13.5-Test Program for Certain Commercial Items; and FAR Part 12-Acquisition of Commercial Items. This acquisition is NOT expected to exceed the simplified acquisition threshold of $250,000.00. A Fixed-Price type of contract is contemplated for any resulting contract entered into as a result of this solicitation. (iii) The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Regulation (FAR) Federal Acquisition Circular (FAC) Number/Effective Date: 2019-05 / 08-13-2019. (iv) The North American Industry Classification System (NAICS) code for this procurement is 541990- All Other Professional, Scientific and Technical Services with size standard $15 million. This requirement is released with no set-aside restriction. (v)This requirement is for the National Center for Advancing Translational Sciences (NCATS) at the National Institutes of Health (NIH) to obtain cell lines utilizing Cas-CRISPR gene editing technology. NCATS brings together a diverse range of scientists, including medicinal chemists, biologists, toxicologists, and engineers, in order to ultimately translate basic science into real products and services that help improve people's lives. Included in this process is the development of unique small molecules as potential anticancer agents. NCATS is supporting a basket trial gene therapy project for heredity disorders of vitamin B12 (cobalamin) and methylmalonic acid metabolism (MMA). One mission critical objective is to develop cellular models of metabolism of cobalamin associated B (MMAB) deficiency and mMut deficiency. Knock-out HEK293 and HepG2 cell lines of the MMAB and methylmalonyl-CoA mutase (MUT) genes are needed to accomplish this goal. The studies support the Platform Vector Gene Therapy project supported by the NCATS Cures Acceleration Network (CAN) within the National Center for Advancing Translational Sciences (NCATS) Division of Preclinical Innovation (DPI), Therapeutics Development Branch (TDB). (vi)The purpose of this requirement is to obtain cell lines utilizing Cas-CRISPR gene editing technology. Statement of Work: Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below: Contractor will process and deliver human embryonic kidney (HEK293) and human liver (HepG2) knockout (KO) cell lines to conduct in vitro pharmacology for studies for heredity disorders of vitamin B12 (cobalamin) and methylmalonic acid metabolism (MMA). Specifically, the Contractor will generate HEK293/MMAB, HepG2/MMAB, and HepG2/MUT knockout cell lines utilizing GenCRISPR TM technology in host cell line gRNA-Cas9 plasmid preparation. Each KO cell line will include producing the following deliverables: 1. gRNA and Cas9 expression plasmids; 2. Two bi-allelic KO clones and a negative KO clone. 3. QC Standard: MycoAlert TM PLUS Mycoplasma Detection and cell viability test. 4. Option: Validate the knockout clones to carry the INDELs on CDS at mRNA level by sequencing the RT-PCR product. GOVERNMENT RESPONSIBILITIES: Government will not furnish property, facilities, workspace, computers, or other equipment. Contractor will not use federal facilities. Government will have the responsibility to review and approve the final report. The TDB will be responsible for reviewing the quality control report of each cell line to ensure the following criteria are met: 1) Mycoplasma testing to ensure cells are free from mycoplasma contamination; 2) Sanger sequencing to confirm identity of gene sequence on cell line genome; 3) Cell viability test to ensure cell health; 4) Sequencing of RT-PCR product to validate the KO clones and make sure they carry insertions or deletions on the coding regions at the mRNA level. DELIVERY OR DELIVERABLES: Contractor will provide the data analysis and report for the contents of this statement of work. Reports of cell line test, cell pool examination, cell clone generation, screening of cell clones, validation of clones, and final report shall be submitted at completion of each milestone to be submitted or updated on a status-basis to the Project Officer no less than a monthly basis or when requested. Data Rights: Any data generated as a result of this contract will be the property of NCATS. NCATS shall have unlimited rights to and ownership of all data including reports. In addition, it includes any additional deliverables required by contract change. The definition of "unlimited rights" is contained in Federal Acquisition Regulation (FAR) 27.401, "Definitions." FAR clause 52.227-14, "Rights in Data-General," is hereby incorporated by reference and made a part of this contract/order. Any data schemas will be the exclusive right of the government. Section 508-Electronic and Information Technology Standards: Contractor will not be involved in EIT Standards. No EIT is anticipated to be generated as a result of this requirement. Publications and Publicity: Contractor will not publish results of the work. Confidentiality of Information: Contractor will keep the results of the acquisition confidential. (vii)The Government anticipates award of a firm fixed-price purchase order for this acquisition. The Period of Performance shall commence after Effective Date of Contract. The Period of Performance shall be effective for 20 months including an option to extend services by 2 months to add an additional quantity of validation services as a separately priced line item. Base Year period of performance: 9/15/2019 through 5/14/2021 Optional period of performance: 5/15/2021 through 7/14/2021 A Fixed-Price type of Purchase Order award not exceeding the Simplified Acquisition Threshold is anticipated for any contract resulting from this requirement. The anticipated award date is September 15, 2019. (viii)The provision at FAR 52.252-1, Solicitation Provisions Incorporated by Reference (Feb 1998), applies to this acquisition. This solicitation incorporates one or more solicitation provisions by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. The offeror is cautioned that the listed provisions may include blocks that must be completed by the offeror and submitted with its quotation or offer. In lieu of submitting the full text of those provisions, the offeror may identify the provision by paragraph identifier and provide the appropriate information with its quotation or offer. Also, the full text of a solicitation provision may be accessed electronically at these addresses: https://www.acquisition.gov/browse/index/far https://www.hhs.gov/grants/contracts/contract-policies-regulations/hhsar/index.html (End of provision) The following provisions apply to this acquisition and are incorporated by reference: • FAR 52.204-7, System for Award Management (Oct 2018) • FAR 52.204-16, Commercial and Government Entity Code Reporting (Jul 2016) • FAR 52.212-1, Instructions to Offerors-Commercial Items (Oct 2018) • FAR 52.212-3, Offeror Representations and Certifications-Commercial Items (Oct 2018) • FAR 52.222-52, Exemption from Application of the Service Contract Labor Standards to Contracts for Certain Services-Certification (May 2014) • HHSAR 352.239-73, Electronic and Information Technology Accessibility Notice (December 18, 2015) The clause at FAR 52.252-2, Clauses Incorporated by Reference (Feb 1998), applies to this acquisition. This contract incorporates one or more clauses by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. Also, the full text of a clause may be accessed electronically at these addresses: https://www.acquisition.gov/browse/index/far https://www.hhs.gov/grants/contracts/contract-policies-regulations/hhsar/index.html (End of clause) The following clauses apply to this acquisition and are incorporated by reference: • FAR 52.204-13, System for Award Management Maintenance (Oct 2018) • FAR 52.204-18, Commercial and Government Entity Code Maintenance (Jul 2016) • FAR 52.212-4, Contract Terms and Conditions--Commercial Items (Oct 2018). The provisions of FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items (AUG 2019) is applicable to this solicitation: 52.222-3 Convict Labor (June 2003) 52.222-19 Child Labor-Cooperation With Authorities and Remedies (Jan 2018) 52.222-21 Prohibition of Segregated Facilities (Apr 2015) 52.222-26 Equal Opportunity (Sep 2016) 52.222-36 Equal Opportunity for Workers With Disabilities (Jul 2014) 52.222-50 Combating Trafficking in Persons (Mar 2015) 52.223-18 Encouraging Contractor Policies to Ban Text Messaging While Driving (Aug 2011) 52.232-33 Payment by Electronic Funds Transfer-- System for Award Management (Jul 2013) The following provisions and clauses apply to this acquisition and are incorporated as an attachment: • NIH Invoice and Payment Provisions (2/2014) • FAR 52.204-24, Representation Regarding Certain Telecommunications and Video Surveillance Services or Equipment (Aug 2019) (ix)The Government will evaluate quotations or offers in accordance with FAR 13.106-2 and award a purchase order from this solicitation to the responsible offeror whose quote conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate quotes: a) Technical Capability: Ability of the Offeror to understand the SOW and requirements, adequately describe the proposed approach, identify problems and potential challenges, and demonstrate how all contract requirements will be met. Offerors shall provide evidence of sufficient planning to show that the work will be accomplished and how it will be accomplished within time and other constraints specified in the SOW or as applicable. The Offeror shall utilize all available resources to include controlling the execution of assigned activities, tasks, sub-tasks, monitoring progress, status reporting, resolving critical issues and/or mitigating risks and fulfilling all delivery requirements. b) Price; and c) Past performance [see FAR 13.106-2(b)(3)] Offerors shall show documented evidence of personnel experience related to the subject requirement. Specific and specialized relevant experience, knowledge, skills, abilities, and education of proposed personnel shall be clearly documented. The offeror must submit with their proposal a list of the last two (2) contracts completed during the past three (3) years and / or contracts awarded currently in process that are similar in nature to the work scope. Contracts listed may include those entered into by the Federal Government, agencies of state and local governments and commercial concerns. Newly formed entities without prior contracts should list contracts and subcontracts as required above for such key personnel. For each contract, subcontract, or reference include: (i) Name of Contracting Organization; (ii) Contract Number (for subcontracts, provide the prime and subcontract numbers); (iii) Contract Type; (iv) Total Contract Value; (v) Description of Requirement; (vi) North American Industry Classification System (NAICS) code. Offeror's submitting a response must provide a completed Price Quote to be evaluated and considered for award. Price quotes must indicate fixed unit pricing and total cost, including any shipping cost and estimated time of delivery after receipt of order if applicable. Prices shall be quoted on an itemized basis with a fixed-price pricing structure included for all work to be performed in accomplishing this requirement. (x)The Offerors to include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications-Commercial Items, with its offer. (xi)The clause at FAR 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. (xii)The clause at FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items, applies to this acquisition and is incorporated in full text. (xiii)The following additional contract requirement(s) or terms and conditions as determined by the contracting officer are necessary for this acquisition and consistent with customary commercial practices. There are no additional contract requirement(s) or terms and conditions applicable to this acquisition. (xiv)The Defense Priorities and Allocations System (DPAS) are not applicable to this requirement. (xv)Responses to this solicitation must include clear and convincing evidence of the offeror's capability of fulfilling the requirement as it relates to the technical evaluation criteria. The price proposal must include the labor categories, an estimate of the number of hours required for each labor category, fully loaded fixed hourly rate or each labor category, breakdown and rationale for other direct costs or materials, and the total amount. The Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov and the offeror representation in FAR Provision 52.204-24, which is attached, MUST be completed and submitted with any quotation or response to this RFQ. (xvi)All responses must be received no later than the date specified in this announcement. Late responses will not be accepted. All responses must reference the subject solicitation number. Responses must be submitted electronically to hunter.tjugum@nih.gov. Facsimile responses will not be accepted. For information regarding this solicitation, contact Hunter Tjugum by email at hunter.tjugum@nih.gov or by phone at 301-827-5304.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/75N95019Q00345/listing.html)
- Record
- SN05437287-W 20190911/190909231213-43152e091536f10305d850cc5fd81417 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |