SOURCES SOUGHT
Q -- Measurement of p16 in T cells/CD3+ cells from the Baltimore Longitudinal Study on Aging (BLSA) participant samples.
- Notice Date
- 1/28/2021 6:04:08 AM
- Notice Type
- Sources Sought
- NAICS
- 621511
— Medical Laboratories
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- 75N95021Q00060
- Response Due
- 2/12/2021 12:00:00 PM
- Archive Date
- 02/27/2021
- Point of Contact
- Jeanette Russll, Phone: (301) 827-5140
- E-Mail Address
-
Jeanette.Russell@nih.gov
(Jeanette.Russell@nih.gov)
- Small Business Set-Aside
- SBA Total Small Business Set-Aside (FAR 19.5)
- Description
- This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition.� It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.� Responses will not be considered as proposals or quotes.� No award will be made as a result of this notice.� The Government will NOT be responsible for any costs incurred by the respondents to this notice.� This notice is strictly for research and information purposes only. Background:� This request is for the measurement of p16 T cells/CD3+ cells to support the research of the Baltimore Longitudinal Study on Aging (BLSA) samples in aging participants within the National Institute on Aging (NIA) Laboratory of Clinical Investigation (LCI). Purpose and Objectives: P16 is an important biomarker cellular senescense. In several publications it has been demonstrated that the measurement of p16 T cells/CD3+ cells has clinical utility in identifying individuals at risk for age-related complications. The NIA, LCI will evaluate the cellular senescence from the Baltimore Longitudinal Study on Aging (BLSA) participants. Fresh blood samples will be obtained from the BLSA participants admitted at the NIA at Harbor Hospital, in Baltimore MD. The LCI wet lab team will perform an isolation of the peripheral blood mononuclear cells (PBMCs), and consequentially sort CD3+ cells using magnetic bead bound to specific antibodies. Frozen aliquots of CD3+ cells will be sent from the NIA to the Contractor 4 times a week in a batch of 40 samples each at a total of 160 samples. It is crucial for the Contractor to isolate the T cells/CD3+ as it has been demonstrated that the level of p16 expression in other PBMC populations (e.g. in monocytes) is too low. It is also crucial to sort the T cells/CD3+ from fresh blood vs frozen because it has been demonstrated that PBMCs can be thawed and intact T cells isolated using traditional cell sorting methods. Unfortunately, the levels of p16 expression measured in T cells isolated from frozen PBMCs correlates poorly with levels obtained from T cells isolated from fresh blood, with R2 of only 0.59. The measurement of p16 in T cells/CD3+ from the BLSA participant samples is needed to continue ongoing research. Project requirements: The Contractor shall provide a measurement of p16 in T cells/CD3+ cells using BLSA participant samples. The p16 cells will be measured as p16 mRNA transcripts abundance using quantitative real-time PCR assay. The Contractor shall develop the p16 measurement assay in a CLIA-compliant laboratory test using quality control systems and optimization of analytical performance of the assay; based on clinical performance standards and clinical workflow considerations. The Contractor shall provide everything necessary for the project set-up, tools and reagent (including blood stabilization solution) for T cells/CD3+ isolation, and completion of a p16 qPCR assay by measuring p16 in 160 BLSA participant samples. The contractor shall provide a data report for each sample received and batch run. The data report must include a study rationale and objective, methods used to generate the data, data analysis of the data, rationale used in the data analysis, and provide the study context. The Contractor shall provide information on whether the p16 expression T cells/CD3+ cells from the BLSA participants is in correlation of other studies with participants similar in age. The Contractor shall provide a virtual training for the T cells/CD3+ cells isolation from whole blood to the LCI wet lab team Anticipated period of performance: One year from date of award. Capability statement /information sought. Respondents must provide, as part of their response, a capability statement that clearly identifies information regarding: (a) staff expertise, including their availability, experience, and formal and other training; (b) current in-house capability and capacity to perform the work; (c) prior completed projects of similar nature; (d) corporate experience and management capability; and (e) examples of prior completed Government contracts, references, and other related information. Please note that NIA is particularly interested in small business sources. If you are a small business, please state the type of small business (small business; HUBZone; service-disabled, veteran-owned; 8(a); veteran-owned; woman-owned; or small disadvantaged businesses) and your size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. The respondent must also provide their� DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2� x 11� paper size, with 1� top, bottom, left and right margins, and with single or double spacing. The information submitted must be in an outline format that addresses each of the elements of the project requirements and in the capability statement /information sought paragraphs stated herein.� A cover page and an executive summary may be included but is not required. The response is limited to ten (10) pages.� The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents� technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. All responses to this notice must be submitted electronically to the Contract Specialist.� Facsimile responses are NOT accepted. The response must be submitted to Jeanette Russell, Contract Specialist, at e-mail address Jeanette.Russell@nih.gov. The response must be received on or before February 12, 2021 at 3:00 pm, Eastern Time. �Disclaimer and Important Notes:� This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).�
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/e415db7a952046cba564b590436f0955/view)
- Place of Performance
- Address: Baltimore, MD, USA
- Country: USA
- Country: USA
- Record
- SN05903144-F 20210130/210128230117 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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