SPECIAL NOTICE
66 -- Becton Dickinson Symphony S6 6-laser Flow Cytometer/Cell Sorter
- Notice Date
- 3/23/2021 3:12:49 PM
- Notice Type
- Special Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- NIH NCI ROCKVILLE MD 20852 USA
- ZIP Code
- 20852
- Solicitation Number
- 75N91021Q00053
- Response Due
- 4/6/2021 9:00:00 AM
- Archive Date
- 04/21/2021
- Point of Contact
- Miguel Diaz, Phone: 240-276-5439
- E-Mail Address
-
miguel.diaz@nih.gov
(miguel.diaz@nih.gov)
- Description
- General Information� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � �� Title:�Becton Dickinson Symphony S6 6-laser Flow Cytometer/Cell Sorter Document Type:�Notice of Intent to Sole Source Solicitation Number:�75N91021Q00053 Classification Code:�6640 - Instruments and Laboratory Equipment NAICS Code:�334516 - Analytical Laboratory Instrument Manufacturing Contracting Office Address: Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E144, Bethesda, MD� 20892, UNITED STATES Description: National Cancer Institute (NCI), Center for Cancer Research (CCR), Experimental Immunobiology Branch (EIB) plans to procure Becton Dickinson Special Order Symphony S6 6-laser Flow Cytometer/Cell Sorter from Becton, Dickenson and Company of One Becton Drive Franklin Lakes, NJ 07417 on a sole source basis. This acquisition will be processed under FAR Part 12 � Acquisition for Commercial Items and will be made pursuant to the authority in FAR 13.106-1 (b)(1)(i) using simplified acquisition procedures for commercial acquisitions.� The response close date of this notice for this requirement is in accordance with FAR 5.203(a)(1). The North American Industry Classification System code is 334516 and the business size standard is 1,000 employees. The intent of this requirement is acquisition of a new state-of-the-art 6-laser cell sorter for the Experimental Immunology Branch (EIB) Flow Cytometry Facility. The instrument is intended to replace an obsolete 2009 Becton Dickinson 6-laser cell sorter that supports multiple ongoing research projects in order to maintain state-of-the art instrumentation. The EIB Flow Cytometry Facility provides basic research support to members of the EIB as well as to investigators throughout CCR, NCI. Support is provided for ongoing immunobiology research projects including studies of: thymocyte biology and differentiation, T cell and B cell development, differentiation and activation, mechanisms of gene expression and chromatin biology, mechanisms of neuro-immunological pathology, mechanisms of gut inflammation, antigen processing and presentation, mechanisms of co-stimulation in T and B cell biology.� Currently, support is provided to about 65 users with 5 flow cytometry analyzers and 2 flow cytometry cell sorters manufactured by Becton Dickinson (BD).� All 7 instruments are produced by the same manufacturer (and thus use similar optics, electronics, laser sources and software) so experiments can seamlessly transfer between instruments with identical results. Protocols for cell preparation and staining with fluorescent reagents can be developed on the flow cytometry analyzers and transferred immediately to the cell sorters for cell separation. Each instrument supports multiple experiments each day, and each instrument is fully utilized. Demand for instrument time currently exceeds availability so the ability to transfer seamlessly between instruments is required in order to maintain throughput and to maintain data integrity when experiments need to be transferred to a different cell sorter, due to malfunctions.� Becton Dickinson instrumentation is necessary to provide compatibility with existing instrumentation and established research protocols.�� By replacing the obsolete 6-laser cell sorter with a Becton Dickinson state of the art cell sorter, the two EIB facility cell sorters will share 5 identical laser excitation sources, providing additional instrument redundancy.� Additionally, the new cell sorter shall be able to analyze, and use for cell sorting, additional fluorochromes such that the instrument will support more complex experiments than the current obsolete 2009 cell sorter. Replacing the obsolete cell sorter with a new cell sorter from the same manufacturer will be able to continue long term research projects that now utilize the current cell sorters.� Replacing the current obsolete cell sorter is also required due to it utilizing obsolete biosafety protections with a cell sorter in a self-contained biosafety hood providing the ability to sort Biosafety Level 2 biological materials in accordance with NIH safety requirements. The equipment is a non-standard configuration and will be custom manufactured with a unique laser and fluorescent detector configuration that meets the requirements of the EIB and that is required to continue ongoing research products� The following product features/characteristics are required: Six lasers must include:� 488nm = 200mW, 637nm = 140mW, 405nm = 200mW, 561nm = 150mW, 355nm = 60mW, 592nm = 100mW. All 6 lasers to be air-launched.� This specific excitation platform configuration required by EIB for compatibility with existing instrumentation and with established research protocols using specific fluorescent dyes and seamless movement of experiments between the existing equipment and the new equipment. 35 Fluorescence and 2 Scatter Detectors with appropriate optical filters must be configured as follows for each laser: 488nm blue laser: forward scatter, side scatter, 6 fluorescence detectors. 637 nm red laser:� 3 fluorescence detectors 405nm violet laser:� 8 fluorescence detectors 561nm yellow-green laser: 3 fluorescence detectors 355nm uv laser:� 8 fluorescence detectors 592 yellow laser: 2 fluorescence detectors This detector configuration is required for compatibility with existing instrumentation and ongoing research projects using specific fluorescence dyes that require these detectors to be measured, and for seamless transition of experiments between the existing equipment and the new equipment. Cell Sorting Functionality: Must provide sorting at 25,000 cells/second with 98% purity. Must provide 6-way sorting. Must include automated cell deposition unit for sorting onto slides or into 6, 48, 96 and 384 well plates. Cell sorter must employ automatic sort setup technology that allows the operator to monitor side streams while sorting. This provides for a faster sort set-up providing the operator with a visual display of the sort set-up. Cell sorting functionality required for compatibility with existing instrumentation and ongoing research projects. Automatic sort setup required for compatibility with existing staff training. Cell sorter must have the ability to threshold on multiple parameters simultaneously. This feature is required for detecting small particles or samples with large amounts of debris.Capability for temperature control (heating and cooling) of sample tube and sorted cells. This feature is required to keep cells alive during sorting. Cell Sorter must have automated compensation networks between all lasers and all fluorescence parameters. Cell sorter must provide real time fluorescence compensation during sorting.� Real time compensation required for compatibility with existing multi-color fluorescence cell sorting protocols that support ongoing research projects. Instrument control and data management to be provided by BD FACSDiva software v8,0 or later and to include the ability to produce FCS2.0, 3.0 and 3.1 data files, the ability to ratio any signal from any laser, and the ability to automatically determine and adjust optimal laser delays. FACSDiva software required for compatibility with existing instrumentation, with current operator training, with custom EIB-produced analysis software and with established protocols used in ongoing research projects. Cell sorter must be installed in a fully integrated Class II NSF-49 certified Biosafety Cabinet (BSC) with ULPA filtration system for live cell sorting. Redundant aerosol management system must function in event of blower motor failure. Required to meet NIH safety guidelines for sorting BSL-2 biomaterials. Maximum heat dissipation of cell sorter system in Biosafety Cabinet must be < 5300 BTU/hour.� Biosafety cabinet must be self-contained to operate without connection to building ventilation system.� This requirement will allow new instrument to operate in existing environmental conditions of the laboratory without renovations. Cell sorter installed in Biosafety Cabinet must have maximum dimensions: H 88�, x W 56�, x D 36� in BSC. This requirement required to install equipment in existing laboratory space. Two (2) year manufacturer extended warranty to include emergency repair on-site service by in-state qualified factory trained field engineers with a 48-hour response time. The equipment will be a custom manufacturer instrument of high complexity and an extended warranty is required. A 48-hour response time is required due to the heavy experimental load of ongoing research experiments. This notice is not a request for competitive quotation. However, if any interested party, especially small business believes it can meet the above requirement, it may submit a capability statement for the Government to consider. The response and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement.� Responses must be received in the contracting office by 12:00 PM ET, on April 06, 2021.� All responses and questions must be via email to Miguel Diaz, Contracting Officer at miguel.diaz@nih.gov.� A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement.� In order to receive an award, contractors must be registered and have valid certification through SAM.GOV and have Representations and Certifications filled out. Reference: 75N91021Q00053 on all correspondence.
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/25bcae9cd5ca4cf6be9d3c50f64664ea/view)
- Place of Performance
- Address: Bethesda, MD 20892, USA
- Zip Code: 20892
- Country: USA
- Zip Code: 20892
- Record
- SN05950580-F 20210325/210323230116 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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