AWARD
B -- Pacbio Sequencing of 8 citrus (Pomelo) tissue samples
- Notice Date
- 3/31/2021 4:39:56 AM
- Notice Type
- Award Notice
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- USDA ARS SEA AAO ACQ/PER PROP STONEVILLE MS 38776 USA
- ZIP Code
- 38776
- Solicitation Number
- 12405B21P0080
- Archive Date
- 04/13/2021
- Point of Contact
- Jessica P. Pacheco, Phone: 6626865414
- E-Mail Address
-
jessica.pacheco@usda.gov
(jessica.pacheco@usda.gov)
- Award Number
- 12405B21P0080
- Award Date
- 03/29/2021
- Awardee
- UNIVERSITY OF ARIZONA Tucson AZ USA
- Award Amount
- 37200.00
- Description
- This award is for a Genomics Project of Pacbio Sequencing of 8 citrus (Pomelo) tissue samples that must run together as a batch of 8. Receive 8 tissue samples and extract HMW DNA suitable for Pacbio sequencing a- Must receive correct amount of tissue and quality of tissue for HMW DNA; b- Extractions performed using modified CTAB; QC data available c- cost for shipping is the responsibility of the customer Produce Pacbio HiFi sequence libraries a- Construct one HiFi library from each DNA sample b- HiFi library, size selected 15-25kb c- Amnt of library correct for Number of cells to run Sequence libraries on Pacbio Sequel II; FOR EACH SAMPLE: a- Sequencing Chemistry used is SeqII v2.0; SMRT cells are 8M v1 b- Sequence 1 cell HiFi CCS (30hr) c- Each CCS cell is expected to produce 16-24Gb of QV20+ CCS data. No guarantee on yield. Run CCS analysis on all raw data; summarize data and deliver via secure FTP a- CCS analysis run on each HiFi CCS cell raw data; b- No additional bioinformatics. No Assembly c- AGI QC data, monitor progress, summarize outputs and stats. Deliver CCS data via secure FTP (or cloud). TOTAL COST for 8 sample data sets (from 8 cells sequenced) Includes all materials, supplies, labor, and fees
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/1dd95604423542d7b015e63f298d60d0/view)
- Place of Performance
- Address: Tucson, AZ 85719, USA
- Zip Code: 85719
- Country: USA
- Zip Code: 85719
- Record
- SN05957747-F 20210402/210331230058 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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