SPECIAL NOTICE
B -- Notice of Intent to Sole Source - Whole Genome Sequencing of V. parahaemolyticus isolates in Alaskan Oysters
- Notice Date
- 6/2/2021 10:20:11 AM
- Notice Type
- Special Notice
- NAICS
- 541715
— Research and Development in the Physical, Engineering, and Life Sciences (except Nanotechnology and Biotechnology)
- Contracting Office
- DEPT OF COMMERCE NOAA KANSAS CITY MO 64106 USA
- ZIP Code
- 64106
- Solicitation Number
- 1333M2-21-Q-00965
- Response Due
- 6/17/2021 8:00:00 AM
- Archive Date
- 07/02/2021
- Point of Contact
- Scott Beliveau
- E-Mail Address
-
scott.beliveau@noaa.gov
(scott.beliveau@noaa.gov)
- Description
- The NOAA Cooperative Oxford Laboratory (COL) is working with the Food and Drug Administration�s Center for Food Safety and Applied Nutrition (FDA CFSAN) to investigate Vibrio parahaemolyticus strains in Alaskan oysters. These bacteria are of interest to the aquaculture industry due to the capability of causing human illness, usually in the form of gastroenteritis from eating raw or undercooked seafood. The goal of this project is to investigate which strains are present in Alaskan shellfish/coastal waters to inform the industry and regulatory officials of potential risks of contamination from pathogenic strains, as well as to investigate migration of strains from the Pacific Northwest to Alaskan waters. Sampling for this project was completed in September of 2018 and 56 V. parahaemolyticus isolates were cultured in preparation for Next Generation Sequencing (NGS) analysis. Whole genome sequencing (WGS) of these isolates is required for comprehensive understanding of the strains present in the Alaskan oysters sampled, and will enable NOAA to inform stakeholders of Vibrio migrations and potential pathogenic strains isolated from Alaskan aquaculture sites.�Whole Genome Sequencing must be completed using Illumina platforms (Miseq or Novaseq instrument) for results to be congruent with current FDA sequencing methods. Additionally, the results require a quality guarantee of > 80% of bases > Q30.� Whole Genome Sequencing shall be conducted from both purifiied genomic DNA and frozen bacterial cells.�
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/e76287439d1047a49f86cfbc3cdeee9a/view)
- Record
- SN06018715-F 20210604/210602230116 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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