SOURCES SOUGHT
66 -- Fully Automated Exosome Characterization Platform
- Notice Date
- 6/11/2021 6:56:27 AM
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-75N95021Q00229
- Response Due
- 6/28/2021 7:00:00 AM
- Archive Date
- 07/13/2021
- Point of Contact
- Karen Mahon
- E-Mail Address
-
Karen.Mahon@nih.gov
(Karen.Mahon@nih.gov)
- Description
- This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition.� It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.� Responses will not be considered as proposals or quotes.� No award will be made as a result of this notice.� The Government will NOT be responsible for any costs incurred by the respondents to this notice.� This notice is strictly for research and information purposes only. Background:� NIA has provided initial proof of concept that exosomes isolated from plasma effectively discriminate between Alzheimer�s disease patients and controls when investigating certain biomarkers. Studying exosome field requires extensive characterization of exosome and this process is the one of the key part our biomarker studies. Following initial successful results in developing a diagnostic test for prediction of Alzheimer�s disease, NIA plans to validate these biomarkers in larger cohorts and expand its application to different neurodegenerative disorders. Apart from that, NIA plans to use not only biomarkers originating from neuronal cells but other cell origins such as astrocytic, oligodendrocytic etc. Each subpopulation maintaines the caracteristics of tissue of origin which is unique in expression of proteins on their sufface as well as the cargo encapsulated inside the plasma membraine. To discover and characterize novel biomarkers, NIA needs to rely on new approaches and technologies. Purpose and Objectives: This platform is a general requirement for the ongoing and future extracellular vesicle (EV) studies involving the biomarker detection and discovery and characterization of EVs isolated from blood samples of both humans and rodents for Cellular & Molecular Neuroscience Section. Currently, NIA does not have a fully automated platform that allow us to simultaneuos characterization and biomarker detection with low volume of EV samples. NIA requires this fully automated platform to quantify, characterize cell specific EVs isolated from plasma samples and identify different subclasses of EVs for functional studies using rodent models and cell culture systems. Project requirements: Fully Automated Exosome characterization platform. Salient characteristics include: Capabilities of measurements of exosome size, concentration, phenotype, and biomarker (proteins, nucleic acids) detection, identification and colocalization. Ability to measure up to 4 markers on single exosomes and other extracellular vesicles. Ability to measure protein and nucleic acid targets. �identification and co-localization of markers specific to both surface and cargo proteins of extracellular vesicles. customization and multiplexing - simultaneous detection of multiple protein or nucleic acid targets. Up to 6 markers to be probed in parallel through binding of the exosomes to an antibody containing chip. The addition of fluorescent antibodies provides the ability to probe for an additional 3 markers with single fluorescent antibody sensitivity. Specifications: Minimum Particle Size Scatter 50nm Concentration Linearity 5x105 to 1x108 particles/mL Fluorescence Sensitivity Better than 10 fluorescein equivalents Excitation Wavelengths:� 410nm (interferometry) 488nm (fluorescence) 555nm (fluorescence) 640nm (fluorescence Anticipated delivery: 9 weeks after receipt of order Other important considerations: NIA also requires an optional annual calibration and performance qualification, to begin 12 months after receipt of order Capability statement /information sought. Respondents must provide, as part of their responses, a capability statement that demonstrates their ability to provide a product that meets the requirements described above. The respondent must also provide their� DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2� x 11� paper size, with 1� top, bottom, left and right margins, and with single or double spacing. The information submitted must be in an outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein.� A cover page and an executive summary may be included but is not required. The response is limited to ten (10) page limit.� The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents� technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer.� Facsimile responses are NOT accepted. The response must be submitted to Karen Mahon, Contracting Officer, at e-mail address Karen.Mahon@nih.gov. The response must be received on or before June 28, 2021 at 10:00 am, Eastern Time. �Disclaimer and Important Notes:� This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).�
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/c8d671ecc0d54f82993faa439ce9917b/view)
- Record
- SN06029851-F 20210613/210611230116 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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