SOLICITATION NOTICE
66 -- PURCHASE OF 200 16S RRNA MICROBIAL AND 60 CYTOCHROME C OXIDASE 1
- Notice Date
- 6/24/2021 4:58:51 PM
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- DEPT OF COMMERCE NOAA SEATTLE WA 98115 USA
- ZIP Code
- 98115
- Solicitation Number
- NFFP72102102687SRG
- Response Due
- 6/29/2021 1:00:00 PM
- Archive Date
- 06/30/2021
- Point of Contact
- SUZANNE ROMBERG-GARRETT, Phone: 3034975110, Fax: 3034973163
- E-Mail Address
-
SUZANNE.GARRETT@NOAA.GOV
(SUZANNE.GARRETT@NOAA.GOV)
- Small Business Set-Aside
- SBA Total Small Business Set-Aside (FAR 19.5)
- Description
- AMENDMENT 6/24/2021 QUESTIONS: ""To be summarized, you want to do 16s rRNA sequencing for 200 DNA samples and COI sequencing for 60 samples, right? Please confirm. We will use one MiSeq lane for these 260 samples.� For�COI sequencing, Our supplier is not sure why you want to do two PCR rounds, actually, one PCR using�BF2+BR2 with in-line tags & Illumina sequencing adaptors will be good enough, as we will do with the 16s sequencing, please let me know if this is fine with you."" ANSWER:� Nope, that isn't fine for the CO1.� We are following the protocol of the reference cited in the tech specs, which uses a 2-PCR process to reduce tag switching that occurs with the single PCR.� First step has no tags, while the 2nd step does.� This is recommended by colleagues working with similar taxa. COMBINED SYNOPSIS/SOLICITATION� PURCHASE OF 200 16S RRNA MICROBIAL AND 60 CYTOCHROME C OXIDASE 1 SAMPLES HIGH THROUGHPUT SEQUENCING FOR LOWER TROPHIC LEVELS IN MARINE WATERS IN ACCORDANCE WITH THE ATTACHED STATEMENT OF NEED (I)����������� This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice and in accordance with the simplified acquisition procedures authorized in FAR Part 13. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. (II)��������� This solicitation is issued as a request for quotation (RFQ).� Submit written quotes on RFQ Number NFFP72102102687SRG. (III)������� The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2021-05 (MAR 2021) (DEVIATION 2020-11) (AUG 2017). (IV)�������� This solicitation is being issued as a Total Small Business Set-Aside. The associated NAICS code is 334516.� The small business size standard is 1000 employees. (V)��������� This combined solicitation/synopsis is for purchase of the following commercial services: CLIN 0001: Purchase of a quantity of 200 samples, 16S rRNA microbial, Primer of 515fB - GTGYCAGCMGCCGCGGTAA; 806rB � GGACTACNVGGGTWTCTAAT (updated EMP pair), Amplicon size of 292 bp and reference is Earth Microbiome Project 16S Illumins Protocol (https://earthmicrobiome.org/protocols-and-standards/16s/) $__________ per sample and extended price for 200 samples $__________. CLIN 0002: Purchase a quantity of 60, Cytochrome c oxidase 1, primer of BF2 - GCHCCHGAYATRGCHTTYCC BR2 -� TCDGGRTGNCCRAARAAYCA, amplicon size of 424 bp and reference of� Elbrecht & Steinke, 2019.� Note this is a 2-PCR library prep with 1st PCR for amplification using unmodified BF2 + BR2, & 2nd PCR using BF2+BR2 with in-line tags & Illumina sequencing adaptors.� No cleanup between 1st & 2nd PCR.� Will need to synthesize ten (10) primers:� unmodified BF2, unmodified BR2, four (4) modified BF2, four (4) modified BR2 (Fig 3 of reference) $__________ per sample and extended price for 60 samples $__________. Total of both CLIN 0001 & 0002 $___________________
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/2dd66629ff8b44d3baa7bd8dc3542c76/view)
- Place of Performance
- Address: Seattle, WA 98112, USA
- Zip Code: 98112
- Country: USA
- Zip Code: 98112
- Record
- SN06042266-F 20210626/210624230122 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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