Loren Data's SAM Daily™

SAMDAILY.US - ISSUE OF JULY 01, 2021 SAM #7152
SOLICITATION NOTICE

66 -- Reagents for library preparation

Notice Date

6/29/2021 5:49:01 PM
 

Notice Type

Solicitation
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

NAVAL MEDICAL RESEARCH CENTER DET DPO AA 34031-0008 USA
 

ZIP Code

34031-0008
 

Solicitation Number

VEID210104
 

Response Due

7/13/2021 9:00:00 AM
 

Archive Date

07/28/2021
 

Point of Contact

Genaro M Vasquez, Phone: 0115116144128
 

E-Mail Address

genaro.m.vasquez.ln@mail.mil
(genaro.m.vasquez.ln@mail.mil)
 

Description

The Virology & Emerging Infections Department of the U.S. Naval Medical and Research Unit No. 6 (NAMRU-6) is looking for reagents to prepare libraries using human and animal samples that later will be sequenced in the MiSeq platform developed by Illumina, as part of the projects of next-generation sequencing (NGS) that are being conducted at this command. The kits must be designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime end for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run. Items requested must be shipped in two installments: first shipment must be effected upon award signature; and second one must be effected four months afterwards. This timeline is important since these reagents are very time-sensitive and have limited expiration dates.
 

Web Link

SAM.gov Permalink
(https://beta.sam.gov/opp/ad9fb09c947c4f5ea02ca15404eda63f/view)
 

Place of Performance

Address: PER

Country: PER
 

Record

SN06046552-F 20210701/210629230152 (samdaily.us)
 

Source

SAM.gov Link to This Notice
(may not be valid after Archive Date)

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