SOLICITATION NOTICE
Q -- SEQUENCING LIBRARY
- Notice Date
- 7/5/2021 12:37:32 PM
- Notice Type
- Presolicitation
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NICHD BETHESDA MD 20817 USA
- ZIP Code
- 20817
- Solicitation Number
- NICHD-21-167
- Response Due
- 7/12/2021 8:00:00 AM
- Archive Date
- 07/13/2021
- Point of Contact
- Verne L. Griffin, Mr. Kyle Wisor, Phone: 3014350950
- E-Mail Address
-
verne.griffin@nih.gov, kyle.wisor@nih.gov
(verne.griffin@nih.gov, kyle.wisor@nih.gov)
- Description
- PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS INTRODUCTION THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION. The National Institutes of Health, National Institute of Child Health and Development (NICHD), in support of the work of the National Institute of Diabetes, Digestive, and kidney Disease �(NIDDK), intends to award a purchase order without providing for full and open competition to SEQMATIC 44846 Osgood Road Fremont, CA 94539 to acquire Ten 10X Genomics single cell RNA libraries. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE The intended procurement is classified under NAICS code 541380 with a Size Standard $15.0 M. REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses in effect through the Federal Acquisition Regulation (FAR) Circular (FAC) 2021-05 effective 03-10-202.� This acquisition is conducted under the procedures as prescribed in FAR subpart 13-Simplified Acquisition Procedures at an amount not exceeding the simplified acquisition threshold ($250,000) and FAR Subpart 12- Acquisition of Commercial Items.� STATUTORY AUTHORITY This acquisition is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13�Simplified Acquisition Procedures, Subpart 13.106-1 (b) (1), Soliciting from a single source and is not expected to exceed the simplified acquisition threshold.� Contracts awarded using FAR Part 13�Simplified Acquisition Procedures are exempt from the requirements of FAR Part 6�Competition Requirements. Place of Performance National Institutes of Health 9000 Rockville Pike Phoenix, AZ DESCRIPTION OF REQUIREMENT The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement for single cell RNAseq for more samples in to continue mapping the entire transcriptome including very rare transcripts. DMGS has been collecting samples from iPSC lines (stem cells) during different stages of differentiation. ��More RNA sequencing is required to analyze more time points during iPSC development. The goal of the project is to map the entire transcriptome including very rare transcripts. The 10x Genomic single cell samples are from three different iPSC lines (stem cells) during different stages of differentiation.� To compare these different stages and detect very rare transcripts, RNASEQ paired end with 50bp reads is needed. Data analysis is to accompany these RNAseq assays. Description Ten 10X Libraries������������������������������������������������������������������������������� ����������� SL-032 ���������� Sequencing Library Normalization (per pool) � For 8-23 libraries��� ����������� SQ-003 ���������� 000000-1912-874 Protect premium Research Microscope mot������ ����������������������� SS-068 ����������� Illumina Library qPCR QC of one sample or library pool 1040��� ����������� NovaSeq v1.5, S4 200 cycles (per Flow Cell) � Read length: R1 28 + R2 91bp SB-10X-count� Bioinformatic Analysis (per sample) � Pipeline: cell ranger count (10 EA) Five 10X libraries SL-032������������ Sequencing Library Normalization (per pool) � For 3-7 libraries SQ-003����������� Illumina Library qPCR QC of one sample or library pool SS-065������������ NovaSeq V1.5, S2 100 cycles (per Flow Cell) � Read length: R1 28 + R2 91bp SB-10X-count� Bioinformatic Analysis (per sample) � Pipeline: cell ranger count (5 EA) CONTRACTING WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-� NAME) DETERMINATION The determination by the Government to award a contract without providing for full and open competition is based upon the market research conducted as prescribed in FAR Part 10�Market Research as determined that �SeqMatic LLC is the sole provider of the required sequencing services.� To maintain the integrity of the analysis, the sequencing should be done by the same platform and data format.� To do otherwise, would introduce variables and corrupt the data.�. We need these single cell RNASEQ assays to be comparable to previous RNASEQ data, using same platform and data format.� SEQMATIC is the sole source of 10X single cell RNASEQ for iPSC comparison. CLOSING STATEMENT This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provided feedback to respondents with respect to any information submitted.� Responses to this notice shall contain sufficient information to establish the interested parties� bona-fide capabilities for fulfilling the requirement, unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.� A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. �All responses to this notice shall be submitted electronically by 11:00 AM Eastern Standard Time, on July 12, 2021.
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/3902a5d93f0a40c1a2aaab34b73c9db4/view)
- Record
- SN06051108-F 20210707/210705230104 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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