SPECIAL NOTICE
65 -- The procurement of reagents for single cell DNA sequencing.
- Notice Date
- 7/9/2021 9:37:33 AM
- Notice Type
- Special Notice
- NAICS
- 541714
— Research and Development in Biotechnology (except Nanobiotechnology)
- Contracting Office
- NIH NCI ROCKVILLE MD 20852 USA
- ZIP Code
- 20852
- Solicitation Number
- 75N91021Q00142
- Response Due
- 7/13/2021 9:00:00 AM
- Archive Date
- 07/28/2021
- Point of Contact
- Elizabeth Tucker, Jolomi Omatete
- E-Mail Address
-
elizabeth.tucker@nih.gov, jolomi.omatete@nih.gov
(elizabeth.tucker@nih.gov, jolomi.omatete@nih.gov)
- Description
- 1.0������� DESCRIPTION� The U.S. Department of Health and Human Services (DHHS), National Institutes of Health (NIH), National Cancer Institute (NCI), Center for Cancer Research, HIV and AIDS Malignancy Branch (HAMB) intends to procure drugs and biologicals on a sole source basis from Mission Bio located at 6000 Shoreline Ct. Suite 104, South San Francisco, California 94080. The response close date of the notice for this requirement is in accordance with FAR 5.203(b). This acquisition will be processed under FAR Part 12 - Acquisition for Commercial Items and will be made pursuant to the authority in FAR Part 13.106-1(b)(1); and is exempt from the requirements of FAR Part 6. The North American Industry Classification System code is 541714 with a size standard in number of employees is 1,000. � The anticipated period of performance shall be 12 months from the date of award. The anticipated award shall be a firm fixed price purchase order. It has been determined there are no opportunities to acquire green products or services for this procurement. 2.0 BACKGROUND The HIV and AIDS Malignancy Branch, in the National Cancer Institute, National Institutes of Health, Dept. of Health and Human Services requires the purchase of reagents for single cell DNA sequencing. We have an ongoing project to study cell types and DNA mutations in diseased clinical samples obtained from patients enrolled in clinical trials the NIH. We need to process clinical samples using this new technology for this translational project. 3.0 TYPE OF ORDER This is a Firm Fixed-Price Purchase Order. 4.0 SPECIAL ORDER REQUIREMENTS 4.1�PRODUCT FEATURES/SALIENT CHARACTERISTICS Reagents requested include: 1. Single cell DNA and protein kit to capture single cells and cell surface proteins. 2. DNA Cartridge kit that is compatible with NIH Tapestri instrument. 3. DNA custom oligonucleotide pool to query a custom set of human and viral DNA targets. To be compatible with existing instrumentation (single cell DNA sequencing), the following product features/characteristics listed below are required. � The platform (system of reagents and equipment) must: utilize droplet microfluidic technology to rapidly encapsulate, process, and profile 5,000 to 10,000 individual cells for multi-analyte detection including single-cell DNA and Protein analysis. utilize a unique two-step workflow, including (1) cell encapsulation and protease incubation to lyse cells and allow access to DNA within individual droplets and (2) barcode addition and reagent introduction for target identification and amplification. allow for target amplification without use of whole genome amplification methods (e.g. multiple displacement amplification). have the capability to do both cell encapsulation and barcode/reagent addition on the same cartridge. take no more than 4 hours of hands-on time from cell suspension to sequencing-ready libraries. be compatible with an Illumina sequencing platforms MiSeq System, Illumina HiSeq 2500 System, Illumina Novaseq System. allow for robust DNA detection of single-nucleotide variants (SNVs), small insertion/deletions (indels), and copy number aberrations across regions targeted by gene specific primers. capable of operating in a mode to perform targeted DNA detection and detect protein analytes from the same cell. capable of detecting subclones present in at least 1% of the population. � 4.2 DELIVERY / INSTALLATION The items shall be delivered within 8 weeks of the purchase order award. Delivery will be to the following address: National Institutes of Health 10 Center Dr. Room 5A21 Bethesda, MD 20892 4.3 ������ WARRANTY The Contractor shall warrant that any instruments, equipment, components, or other supplies (�Supplies�) procured by this purchase are free of defects in design, material, or manufacture for a period of at least one (1) calendar year from date of acceptance by the Government. The Contractor shall warrant that all consumables or expendable parts are free of defects in design, material or manufacture for a period of at least 90 calendar days from date of acceptance by the Government. Supplies which�are repaired or replaced shall be warranted for the remainder of the initial warranty period or for 90-days � whichever is greater. 4.4������� PROCEDURES DURING COVID-19 PANDEMIC Due to the current COVID-19 pandemic, the Contractor must pay close attention to the following delivery instructions. Please be aware that due to the fluidity of the existing pandemic, instructions may be updated as needed after award. The equipment shall be delivered within the timeframe listed in section 4.2 above. The Contractor shall: Store or hold equipment until NCI is ready to accept the equipment at no additional cost; The equipment shall not be sold to another business in the event NCI is not ready to accept delivery due to unanticipated changes related to COVID-19; and Training as required by the Statement of Need shall be made available and provided to required staff at different times and dates, if required, at no additional cost. Communicate and follow instructions from the TPOC to coordinate delivery, installation and training in accordance with the timeframes stated in sections 4.2 through 4.3 above. The delivery address and TPOC�s information will be provided at the time of award and must be included as part of the order/shipping label. All companies delivering to NIH�s main campus shall comply with Commercial Vehicle Inspection Facility (CVIF) access procedures. CVIF will provide a list of buildings with loading docks. Delivered items will be logged in and the loading dock Contractor or Government employee will attempt to contact the TPOC for inspection and acceptance of the package. In the event the TPOC is unable to accept the package, the equipment shall not be left at the loading dock station. The Contractor shall confirm another attempt will be made to deliver on another date, to be coordinated with the TPOC, at no additional cost. � Deliveries shall only be made between 6AM to 4PM, Monday through Friday. Note: Delivery personnel may be asked a series of questions related to recent travels and if any physical symptoms relevant to COVID 19 exist. 5.0 INSPECTION AND ACCEPTANCE CRITERIA Pursuant to FAR clause 52.212-4, all work described in the SOW to be delivered under this purchase order is subject to final inspection and acceptance by an authorized representative of the Government. The authorized representative of the Government is the NCI TPOC, who is responsible for inspection and acceptance of all services, materials, or supplies to be provided by the Contractor. Regardless of format, all digital content or communications materials produced as a deliverable under this purchase order, shall conform to applicable Section 508 Standards to allow Federal employees and members of the public with disabilities to have access to and use of information and data that is comparable to the access to and use of information and data by Federal employees or by members of the public who are not individuals with disabilities. Remediation of any materials that do not comply with the applicable Section 508 requirements as set forth below, shall be the responsibility of the Contractor. HHS guidance regarding accessibility of documents can be found at https://www.hhs.gov/web/section-508/making-files-accessible/create-accessible-pdfs/index.html.� Federal government-wide guidance regarding accessibility of documents can be found at�https://www.section508.gov/, including the documents describing the preferred method of authoring and testing documents produced in Microsoft Word 2013 or later, Microsoft Excel, and files formatted as PDF. 6.0������� UNIQUE QUALIFICATIONS HAMB lacks the ability to perform single cell DNA sequencing, which is required for the next step of our ongoing project. We need the ability to mark individual cells with nucleic acid barcodes, then measure both viral and human DNA copy number variations and single nucleotide variants and combine the DNA measurements with surface protein measurements. This is the only system that we have found that can combine these abilities. Furthermore, this system of reagents works with existing equipment at an NCI Frederick core facility. These reagents and technology are new and not available from other suppliers. This is the only supplier of these reagents and the other unique required qualifications are: The platform must utilize droplet microfluidic technology to rapidly encapsulate, process, and profile 5,000 to 10,000 individual cells for multi-analyte detection including single-cell DNA and Protein analysis. The platform must utilize a unique two-step workflow, including (1) cell encapsulation and protease incubation to lyse cells and allow access to DNA within individual droplets and (2) barcode addition and reagent introduction for target identification and amplification. The platform must allow for target amplification without use of whole genome amplification methods (e.g. multiple displacement amplification). The platform must have the capability to do both cell encapsulation and barcode/reagent addition on the same cartridge. The workflow must take no more than 4 hours of hands-on time from cell suspension to sequencing-ready libraries. The platform must be compatible with Illumina sequencing platforms. The platform must allow for robust DNA detection of single-nucleotide variants (SNVs), small insertion/deletions (indels), and copy number aberrations across regions targeted by gene specific primers. The platform must be capable of operating in a mode to perform targeted DNA detection and detect protein analytes from the same cell. The platform must be capable of detecting subclones present in at least 1% of the population. 7.0������� SUBMISSION INFORMATION This notice is not a request for competitive quotation. However, if any interested party, especially small businesses, believes it can meet the above requirement, it may submit a capability statement, proposal, or quotation for the Government to consider.� The statement of capabilities and/or any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement.� All responses must be submitted via email to Contract Specialist, Elizabeth Tucker, at elizabeth.tucker@nih.gov by no later than 12:00 PM EST on July 13, 2021. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractors must be registered and have valid certification in the System for Award Management (SAM) through sam.gov. Reference: 75N91021Q00142 on all correspondence.
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-
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- Record
- SN06056303-F 20210711/210709230113 (samdaily.us)
- Source
-
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