SOLICITATION NOTICE
R -- lab work and research services for the NIDDK
- Notice Date
- 12/13/2021 9:24:23 AM
- Notice Type
- Presolicitation
- NAICS
- 541690
— Other Scientific and Technical Consulting Services
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NICHD BETHESDA MD 20817 USA
- ZIP Code
- 20817
- Solicitation Number
- 75N94022Q00037
- Response Due
- 12/17/2021 6:00:00 AM
- Point of Contact
- Amber Harris, Fax: 3014803278
- E-Mail Address
-
amber.harris@nih.gov
(amber.harris@nih.gov)
- Description
- INTRODUCTION THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). The National Institutes of HealthO, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Office of Acquisitions (OA) on behalf of the on behalf of the National Institute of Digestive, Diabetes & Kidney Diseases intends to award a purchase order without providing for full and open competition (Including brand-name) to EDWAN LLC for lab work and research services for the NIDDK Biomedical and Metabolic Imaging Branch. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE The intended procurement is classified under NAICS code 541690 with a Size Standard of $16.50. REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular 2022-01 Effective December 6, 2021. This acquisition is conducted under the procedures as prescribed in FAR subpart 13�Simplified Acquisition Procedures at an amount not exceeding the simplified acquisition threshold ($250,000). STATUTORY AUTHORITY This acquisition is conducted under the authority of 41 U.S.C. 253(c) under provisions of the statutory authority of FAR Subpart 6.302- FAR 6.302-1�Only one responsible source and no other supplies or services will satisfy agency requirements 41 U.S.C. 253(c)(1). PERIOD OF PERFORMANCE 1/20/2022 - 1/19/2023 Place of Performance National Institutes of Health� National Institute of Digestive, Diabetes & Kidney Diseases� 10 Center Drive Bethesda, MD 20892 � ? DESCRIPTION OF REQUIREMENT Much of the work carried out in the Biomedical and Metabolic Imaging Branch focuses on developing noninvasive imaging approaches with high spatial (micro-millimeter) and temporal (milliseconds) resolution that allow multi-organ evaluation and analysis to better understand the interplay between atherogenesis and metabolic syndrome in humans. This is being achieved through new chemical spectroscopic techniques of involved organs (e.g. liver, heart and muscles) and improved imaging methodologies such as the development of free breathing methods (important for kids and adolescents) and high contrast techniques without the use of contrast agents. The Biomedical and Metabolic Imaging Branch has championed the creation of a multidisciplinary team of imaging physicists, spectroscopists, and biologists. Patients with rare genetic mutations such as hyper immunoglobulin E (Job's) syndrome (immune system and coronary arteries), Turner's syndrome (genetics and vascular disease), lipodystrophy (metabolic dysfunction and cardiovascular disease), or HIV infection (inflammation and coronary disease), share many of the metabolic and immunological dysregulations encountered in multi-organ disease subjects inclusive of atherogenesis. T-cell dysfunction mirrors that seen in obesity affecting leptin metabolism. Finally, it is well established that such subjects acquire accelerated atherosclerosis. Therefore, a goal has been to isolate and understand the effects of these defects and the early events that signal atherosclerosis. Taking advantage of the effects of deregulation of metabolism, inflammation and immunity that play a key role in multi-organ disease progression of atherosclerosis in obesity and metabolic syndrome requires an in-depth understanding of the molecular basis of inflammatory diseases and studying their associated pro-inflammatory cytokines. Specifically, understanding the initiating pathways of the immune system, such as the Toll-Like Receptors (TLRs) and the inflammasome, to determine effective therapies involved in the dysregulation of these inflammatory signals as a treatment approach to ameliorate atherosclerosis and obesity, diabetes and hereditary autoinflammatory diseases. � Novel role for mitochondrial STAT3 has been implicated in autoinflammatory diseases with an underlying component of mitochondrial dysfunction, such as Type 2 diabetes and obesity among many other diseases. Therefore, a deeper understanding of the interplay between STAT3 and mitochondria creates a great advantage to developing therapeutic approaches to treat inflammatory diseases. Research endeavors will employ a combination of flow cytometry, cell imaging, proteomic and bioinformatic approaches to further dissect mechanisms involved in mitochondrial regulation of inflammatory diseases. The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), Biomedical and Metabolic Imaging Branch has a requirement to obtain services of a scientist with extensive expertise in innate immunology and hereditary autoinflammatory diseases with demonstrated understanding of, and ability to apply principles, concepts, practices and standards associated with patient sample testing in a laboratory setting. _________________________ PURPOSE AND OBJECTIVES The National Institutes of Health (NIH)�s National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement for the services of a scientist with specialized skills beyond what our current resources can provide in order to accomplish the goals of this program. The contractor will work 40 hours per week, Monday through Friday, totaling 2080 hours per year.� � SALIENT / REQUIRED FEATURES AND SPECIFICATIONS Specifically, the NIDDK requires the following of the candidate: ��� �PhD with 15+ years of directly relevant experience in immunology, or inflammation. ��� �Proficiency in T cell culture, multicolor Flow Cytometry, RNA-seq, western blotting, q-PCR, multiplex ELISA, RNA/DNA isolation, T cell isolation, cell assay development, mouse handling/ organ isolation is required. ��� �Expertise in lentiviral production, synthetic biology, lentiviral vector design desired. ��� �Expertise in exosome purification and characterization. � ��� �Knowledge of standard Human T cell isolation & in vitro culture techniques is required. ��� �Possess experience using autoimmune and inflammatory disease mouse and rabbit models. ��� �Good understanding of immune signaling pathways and interest in metabolomics. ��� �Ability to contribute creatively and strategically to in vitro assay design, optimization and derive actionable biological conclusions. ��� �Ability to define and implement critical studies needed to support hypothesis-based research. ��� �Ability to write protocols and study reports, produce and maintain accurate records. ��� �Expertise with data analysis software such as Excel and Graphpad Prism ��� �Strong verbal and written communication skills. ��� �Ability to synthesize complex scientific problems into a strategy and tactics. ��� �Familiarity with both Microsoft and Mac operating systems, including PowerPoint, Excel, Word, Adobe Photoshop and Access. ��� �Excellent interpersonal, analytical, organizational and time management skills. The contractor is expected to deliver scientific data and results and implement the following: ��� �Tissue processing and purification of immune cells, in vitro cell based bioassay development, tissue cell culture (primary mouse and human cells, cell lines and genetically engineered cell lines) ��� �Multicolor flow cytometry (FACS), and ELISA assays ��� �Basic molecular biology techniques (cloning, viral tranductions, etc.) ��� �Analyze biological data using FlowJo, Microsoft Excel, and GraphPad Prism ��� �Interpret, analyze and present data; work in fast-paced, result oriented research environment ��� �Contribute to project advancement by developing new methods or technologies and troubleshooting existing protocol ��� �Write protocols and study reports, produce and maintain accurate records ��� �Prepare manuscripts for journal publications TASKS AREAS Independently, and not as an agent of the Government, the contractor shall perform all of the detailed Task Areas listed below: Task Area 1 � Drive discovery and translational research aimed at identifying, characterizing, and interpreting results for novel diagnosis for inflammatory diseases. The candidate must be able to create their own research plans with minimum support from the principal investigator. The candidate must be able to run research plans independently. Weekly/as needed meetings will be held to evaluate the work and provide directions for next steps. Task Area 2 � Define and implement critical studies needed to support hypothesis-based research. The candidate�s progress will be parodically evaluated and the candidate will be provided with feedback and directions. Task Area 3 � Make contributions to scientific literature and conferences through publication and presentation of research results. The candidate will be evaluated based on preparing manuscripts and abstracts for scientific publications. Task Area 4 � Identify and evaluate a wide variety of in vitro assays to test immunological, metabolic, and various disease mechanisms. Task Area 5 � Contribute creatively and strategically to in vitro assay design, optimization, and derive actionable biological conclusions. Task Area 6 � Perform assay development, qualification and validations as needed. Task Area 7 � Conduct cell-based assays including, but not limited to, ELISA and Flow Cytometry. Task Area 8 � Separate, isolate and cryopreserve primary cells peripheral blood mononuclear cells (PBMCs). Task Area 9 � Organize data prior to scientific publication submission. The data will be reviewed and evaluated by the principal investigator. � Task Area 10 � Ensure lab maintenance activities and equipment calibration are performed according to schedule. Task Area 11 � Ensure lab maintenance activities and maintain a clean and safe lab environment. CONTRACTING WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME) DETERMINATION The determination by the Government to award a contract without providing for full and open competition is based upon the market research conducted as prescribed in FAR Part 10�Market Research. �Only one source is available: Per FAR 13.106-1(b)(1) the Contracting Officer has determined EDWAN LLC to be the only reasonable available source to provide lab work and research services for the NIDDK Biomedical and Metabolic Imaging Branch. �� This acquisition was pursued on a sole source basis centered on the fact that this is ongoing project that requires specific knowledge of the history of the development of each experiment and the thought process that led to each conclusion. �Jehad Edwan of Edwan LLC possesses extensive expertise in innate immunology and hereditary autoinflammatory diseases, which is vital to our study of the relationship between atherosclerosis and obesity and other metabolic disorders. CLOSING STATEMENT This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties� bona-fide capabilities for fulfilling the requirement and include: descriptive literature, delivery timeframe, warranties and/or other information that demonstrates that the offer meets all the foregoing requirements, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov.� A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses to this notice shall be submitted electronically by 9:00 am Eastern Standard Time, on Friday, December 17, 2021 to the Contract Specialist, Amber Harris, at amber.harris@nih.gov . Assessment of Capability Lowest Price Technically Acceptable �
- Web Link
-
SAM.gov Permalink
(https://beta.sam.gov/opp/1d37f822c015428bb6a7f970be190e8d/view)
- Place of Performance
- Address: Bethesda, MD 20892, USA
- Zip Code: 20892
- Country: USA
- Zip Code: 20892
- Record
- SN06196407-F 20211215/211213230112 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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