SOURCES SOUGHT
66 -- SOURCES SOUGHT: Single Cell Sequencing Reagent Kits
- Notice Date
- 7/8/2022 1:57:48 PM
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- 75N95022Q00379
- Response Due
- 7/15/2022 8:00:00 AM
- Point of Contact
- Hashim Dasti, Phone: 3014434577, Karen Mahon, Phone: 3014357479
- E-Mail Address
-
hashim.dasti@nih.gov, karen.mahon@nih.gov
(hashim.dasti@nih.gov, karen.mahon@nih.gov)
- Description
- This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement, to determine the method of acquisition, and availability of domestic sources manufactured in the United States in sufficient and reasonably available commercial quantities and of a satisfactory quality.� It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.� Responses will not be considered as proposals or quotes.� No award will be made as a result of this notice.� The Government will NOT be responsible for any costs incurred by the respondents to this notice.� This notice is strictly for research and information purposes only. Small businesses are encouraged to respond. For equipment/supply requirements, responses must include the place of manufacturing (i.e. address if supply/equipment is a domestic end product and include country of manufacture). For equipment/supply requirements, small businesses must also address the size status of the manufacturer under the applicable NAICS code (i.e. address Non-Manufacturer Rule). Background:� It has been hypothesized that senescent cells localize to specific locations within the aorta during aging and atherosclerosis.� Further, the unique markers that identify senescent cell niches may be used to detect senescent cell accumulation in human vascular tissue.� It is important to understand how this senescent cell population operates in the vascular to comprehend its role in aging. A proteomic approach to this question will be of significance in studying what proteins are involved in this complex mechanism.� Primarily focusing on this analysis of atherosclerotic mice will allow us to then determine if these niches can be detected in human tissue. Specifically, NIA will use macro-dissected human aortic samples with sections clinically diagnosed as grossly normal (N) or grossly abnormal (P) from 5 young and 5 old patients. Using single cell sequencing to elucidate these cell characteristics within this population will help further elucidate these processes. Purpose and Objectives: Single Cell Sequencing is becoming more and more valuable as it has become routinely used in research to further elucidate the specific RNA present in a population of single cells. The presence of poly-A tailed RNAs can be amplified and sequenced providing valuable data regarding the presence of multiple RNA species within a single cell which can then be compared to the RNA species present within a population of hundreds to thousands of cells. The purpose of this work will be to understand the context and organization of senescent cells in the vasculature, employing single cell analysis of atherosclerotic mice and attempt to determine if these niches can be detected in human tissue.� The National Institute on Aging (NIA) hopes to determine if senescent populations in human vascular disease and aging reflect the same or similar genotypic markers that can be used to identify senescent cell patterns or populations. Project requirements: The required reagents must be compatible with the 10X Genomics single cell sequencing system, with the ability to process RNA from live cells following the manufacturer�s protocol, with a minimum shelf life of 6 months if stored properly.� The purpose of these reagents will be to create a library specific for each single cell which can then be amplified and sequenced.� The reagents must be able to enter an individual cell, label the RNA with a unique identifier which will be specific for all the labeled RNA in that cell which will be distinguishable from every other cell which will also be labeled with their own unique identifier.� These labeled RNAs must then be further amplified and converted to cDNA libraries which can then be fragmented and sequenced.� The required reagents must be compatible with the 10X Genomics sequencing machinery allowing for formation of GEMS for each individual cell. The Chromium Next GEM Single Cell 3� Kit, v3.1, 16 rxns must have all the required components to perform gene expression single cell sequencing preparations for up to 16 samples.� The kit must include RT Reagent B, RT Enzyme C, Template Sqitch Oligo, Reducing Agent B, Cleanup Buffer, cDNA Primers, Amp Mix. The components of the Library Construction Kit will include Fragmentation enzyme, Fragmentation Buffer, Ligation Buffer, DNA Ligase, Adaptor oligos, and Amp Mix.The kit must also include Single Cell 3� v3.1 Gel Beads. The Chromium Next GEM Chip G Single Cell Kit, 48 rxns must be compatible with the 10X system to correctly generate GEMs for up to 48 samples.� All kits must have the components listed, with expiration dates within 1 year of purchase date.�� Anticipated Delivery Date: 30 days ARO Other important considerations: In case domestic sources are available and capable of fulfilling the Government�s need, and a future solicitation is published, the Government will use evaluation preferences in accordance with FAR 25. Capability statement /information sought. Companies that believe they possess the capabilities to provide the required products should submit documentation of their ability to meet each of the project requirements to the Contracting Officer. The capability statement must specifically address each of the project requirements separately.� Additionally, the capability statement should include 1) the total number of employees, 2) the professional qualifications of personnel as it relates to the requirements outlined, 3) any contractor GSA Schedule contracts and/or other government-wide acquisition contracts (GWACs) by which all of the requirements may be met, if applicable, and 4) any other information considered relevant to this program. Capability statements must also include the Company Name, Unique Entity ID from SAM.gov, Physical Address, and Point of Contact Information. The response must include the respondents� technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. Interested companies are required to identify their type of business, applicable North American Industry Classification System (NAICS) Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2� x 11� paper size, with 1� top, bottom, left and right margins, and with single or double spacing. The information submitted must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein.� A cover page and an executive summary may be included but is not required. The response is limited to ten (10) page limit.� The 10-page limit does not include the cover page, executive summary, or references, if requested. All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer.� Facsimile responses are NOT accepted. The response must be submitted to Hashim Dasti, Contract Specialist, at e-mail address hashim.dasti@nih.gov. The response must be received on or before July 15, 2022 at 11AM Eastern Time. Disclaimer and Important Notes:� This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in www.sam.gov. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).
- Web Link
-
SAM.gov Permalink
(https://sam.gov/opp/542277a456a94c2e9706eafce8aa6f98/view)
- Record
- SN06383332-F 20220710/220708230109 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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