SOURCES SOUGHT
99 -- Nikon Ti2U or Equal
- Notice Date
- 6/5/2023 6:18:24 AM
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- 75N95023Q00375
- Response Due
- 6/15/2023 10:00:00 AM
- Archive Date
- 06/30/2023
- Point of Contact
- Shayna Simpson, Phone: 3018276812, Sneha Singh
- E-Mail Address
-
shayna.simpson@nih.gov, sneha.singh@nih.gov
(shayna.simpson@nih.gov, sneha.singh@nih.gov)
- Description
- This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement, to determine the method of acquisition, and availability of domestic sources manufactured in the United States in sufficient and reasonably available commercial quantities and of a satisfactory quality.� It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.� Responses will not be considered as proposals or quotes.� No award will be made as a result of this notice.� The Government will NOT be responsible for any costs incurred by the respondents to this notice.� This notice is strictly for research and information purposes only. Small businesses are encouraged to respond. Responses must include the place of manufacturing (i.e. address if supply/equipment is a domestic end product and include country of manufacture). For equipment/supply requirements, small businesses must also address the size status of the manufacturer under the applicable NAICS code (i.e. address Non-Manufacturer Rule). The response must also include their DUNS number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. ����������� The purpose of this acquisition is for the Membrane Transport Biophysics section to purchase a microscope system that will allow for patch-clamp electrophysiology and microscopy techniques to be performed simultaneously. The experiments being performed will detect cellular and pH changes along with ionic current across the membrane. To complete these experiments, NINDS will perform membrane potential measurements of cells in patient mutation cell types while looking at localization of proteins and pH changes in the lysosomes. In order to perform these recording, the MTBS needs an inverted, manual microscope in order to collect high quality and patch-clamp equipment to collect electrophysiology data. This new system has to be is compatible with our current microscope system, parts, filters and data acquisition software. This new system will allow for patch-clamp electrophysiology and microscopy techniques to simultaneously detect cellular and pH changes along with ionic current across the membrane, understanding the functions of the lysosome and the role of disease-causing mutations such as in the CLCn7. The Synaptic Functions Section focuses on mechanisms regulating axonal transport that is essential for the maintenance of synaptic function and axonal homeostasis. Using genetic mouse models, the lab is addressing several fundamental questions: (1) how mitochondrial transport is regulated to sense changes in synaptic activity, mitochondrial integrity, axon injury and pathological stress; (2) how neurons coordinate late endocytic transport and autophagy-lysosomal function to maintain cellular homeostasis and distal degradation capacity; (3) how impaired transport contributes to synaptic dysfunction and axonal pathology in neurodegenerative diseases. These studies have led to the identification of three motor adaptor and anchoring proteins (syntaphilin, Snapin, and syntabulin) that regulate axonal transport of mitochondria, endo-lysosomes, autophagosomes, and synaptic cargoes. The long-term goal of the laboratory is to decipher mechanisms (1) boosting axonal and synaptic energy metabolism and (2) enhancing autophagy-lysosomal function for efficient clearance of dysfunctional organelles and aggregated proteins that are associated with major neurodegenerative diseases. Pursuing these investigations will advance our knowledge of fundamental processes that may affect human neurological disorders. The current studies in the lab are heavily reliant on research employing various animal models and tissue samples gathered from these species. Tissue slices are broadly employed in biochemical research to explore protein levels, functions, and interactions, as per the approved animal study protocol.
- Web Link
-
SAM.gov Permalink
(https://sam.gov/opp/edf25c747d344ddeaa240f3b113b1866/view)
- Record
- SN06704646-F 20230607/230605230109 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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