SOURCES SOUGHT
Q -- snRNAseq Analysis of Senolytics in the Aging Kidney and Lung
- Notice Date
- 6/7/2024 12:29:45 PM
- Notice Type
- Sources Sought
- NAICS
- 621511
— Medical Laboratories
- Contracting Office
- NATIONAL INSTITUTES OF HEALTH NIDA Bethesda MD 20892 USA
- ZIP Code
- 20892
- Solicitation Number
- 75N95024Q00336
- Response Due
- 6/24/2024 6:00:00 AM
- Archive Date
- 07/09/2024
- Point of Contact
- Emily Palombo
- E-Mail Address
-
emily.palombo@nih.gov
(emily.palombo@nih.gov)
- Description
- This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS code should not submit a response to this notice. This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition.� It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract.� Responses will not be considered as proposals or quotes.� No award will be made as a result of this notice.� The Government will NOT be responsible for any costs incurred by the respondents to this notice.� This notice is strictly for research and information purposes only. Background:� � Single-cell RNA sequencing (scRNAseq) emerges as an indispensable technique for dissecting the dynamics of senescence induction following drug administration in mice, particularly in organs as diverse as the kidney and lung. The complexity of these tissues requires a cellular-level investigation to unravel the intricacies of senescence dynamics. By employing scRNAseq, The National Institute on Aging (NIA) can delve into the heterogeneous cellular responses, uncovering nuanced changes in gene expression profiles across various cell types over time. This approach not only provides a comprehensive understanding of senescence progression but also unveils potential cell-specific therapeutic targets, advancing the ability to intervene in age-related pathologies. Aging is a multifaceted process that affects all organisms, and understanding its mechanisms is crucial for developing interventions to promote healthy aging. The kidney, a vital organ responsible for filtration and waste excretion, undergoes significant changes with age, leading to functional decline and increased susceptibility to disease. Investigating the dynamics of senescence within the kidney is essential for elucidating the molecular pathways involved in renal aging. Among vital organs, the lungs play a pivotal role in oxygen exchange, facing significant alterations with age, which lead to functional deterioration and heightened disease susceptibility. Exploring the dynamics of senescence within the pulmonary tissue is imperative for unraveling the molecular pathways implicated in pulmonary aging. This project aims to study senescence dynamics in the kidney and lungs of mice by administering doxorubicin, a chemotherapeutic agent. Following doxorubicin administration, mice will be sacrificed at various time points (1, 2, 4, 6, and 12 days), and their kidneys and lungs will be harvested for analysis. NIA will employ a technique known as single nuclei RNA sequencing (snRNA-seq) to examine the transcriptomic landscape of renal cells and pulmonary cells at each time point. These organs are complex and are composed of diverse cell populations, each playing unique roles in maintaining function. Traditional RNA sequencing methods often fail to capture this cellular heterogeneity, leading to a limited understanding of gene expression changes occurring during senescence. snRNA-seq overcomes this limitation by enabling transcriptomic analysis at the single-cell level. By leveraging snRNA-seq, NIA can dissect the distinct transcriptional responses of individual cell types to senescence-inducing stimuli. This information will provide valuable insights into the functional consequences of senescence on different cell populations and identify potential therapeutic targets to mitigate age-related organ dysfunction. Purpose and Objectives: The purpose of this contract is to procure RNA sequencing and data analysis services (following tissue dissociation, nuclei extraction, library preparation) from precious mouse kidney and lung tissues dissected from senolytic treated mice at different time points to identify transcriptomic changes with age and senolytic treatment of these tissues. Project requirements: NIA requires snRNAseq. NIA has already collected and frozen all the samples. The only way to perform single-cell RNAseq in frozen tissue pieces is through the technique proposed here, snRNAseq.�� The services required for this project include: ����������� Nuclei isolation (18 samples) ����������� RNA extraction from the nuclei ����������� Single-nuclei RNA library preparation using 10X Genomics platform ����������� Illumina Nova Seq (250M reads/sample) The vendor shall process the samples and provide sequencing data and analysis within 3 months of receipt of the samples.� The vendor shall provide progress updates during the entire process.� The data and analysis shall be provided electronically. Anticipated period of performance: �3 months after receipt of the samples. Capability statement /information sought. Small business concerns that believe they possess the capabilities to provide the required services should submit documentation of their ability to meet each of the project requirements to the Contracting Officer. The capability statement must specifically address each of the project requirements separately.� Additionally, the capability statement should include 1) the total number of employees, 2) the professional qualifications of personnel as it relates to the requirements outlined, 3) any contractor GSA Schedule contracts and/or other government-wide acquisition contracts (GWACs) by which all of the requirements may be met, if applicable, and 4) any other information considered relevant to this program. Small businesses must also provide their Company Name, Unique Entity ID from SAM.gov, Physical Address, and Point of Contact Information. Interested small businesses are required to identify their type of business, applicable North American Industry Classification System (NAICS) Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number. The respondent must also provide their Unique Entity ID from SAM.gov, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements. One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2� x 11� paper size, with 1� top, bottom, left and right margins, and with single or double spacing. The information submitted must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein.� A cover page and an executive summary may be included but is not required. The response is limited to ten (10) page limit.� The 10-page limit does not include the cover page, executive summary, or references, if requested. The response must include the respondents� technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses. All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer.� Facsimile responses are NOT accepted. The response must be submitted to Emily Palombo, Contract Specialist, at e-mail address Emily.palombo@nih.gov. The response must be received on or before June 24, 2024, 9:00 �am, Eastern Time. �Disclaimer and Important Notes:� This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization�s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).�
- Web Link
-
SAM.gov Permalink
(https://sam.gov/opp/d3631543dc2f4635b9430f3a4785cb8a/view)
- Place of Performance
- Address: Bethesda, MD, USA
- Country: USA
- Country: USA
- Record
- SN07090678-F 20240609/240607230119 (samdaily.us)
- Source
-
SAM.gov Link to This Notice
(may not be valid after Archive Date)
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