National Cancer Institute, Research Contracts Branch, Purchasing Support Contracts Section, EPS/638, 6120 EXECUTIVE BOULEVARD MSC 7228 BETHESDA MD 20892-7228

65 -- AMPLIFICATION OF RECOMBINANT POX VIRUS IN CHICKEN EMBRYO FIBROBLAST CELLS AND DEV OF POLYCLONAL ANTIBODIES TO TUMOR ASSOC ANTIGEN GENE PRODU SOL NCI-CB-56207-84 POC Eileen Keating, Contract Specialist or Janet Mattson, Contracting Officer, 301/402-4509. The National Cancer Institute plans to procure on a SOLE SOURCE basis under authority of FAR 6.320-1, in accordance with 41 U.S.C. 253(c)(1), Amplification of recombinant pox viruses in chicken embryo fibroblast cells and development of polyclonal antibodies to tumor associated antigen gene products from Bio Molecular Technology (BMT) Inc., 5340 Spectrum Drive, Frederick, MD 21701. The items are for the Laboratory of Immunology and Biology (LTIB), for use in an ongoing research project. When the LTIB began using the services of BMT for the development of polyclonal antibodies, they were the only vendor willing to meet our needs by attaching TAA peptides to Keyhole Limpet Hemocyanin (KLH) and the subsequent development and purification of polyclonal antibodies to these peptides. BMT began to provide the service of amplifying and providing purified high titer lysates to several of the LTIB's recombinant pox viruses grown in chicken embryo fibroblast cells. Several vendors were contacted at that time, but none were willing to grow the virus in chicken embryo fibroblast cells for the production of these recombinants except for BMT. The LTIB does not wish to introduce new variables into the ongoing experiments and especially do not wish to cause delays generated by the introduction of the services of a new vendor. To introduce a new vendor at this time would by very costly and would not be in the best interest of the Government since it would delay the development of these novel new therapies for the treatment of human carcinomas. The planned period of performance is 12 months plus 4 successive 12-month periods. Due to the above requirements and the unique capabilities of BMT, there is no other known vender to meet NCI's requirements. It is the Government's belief that the above requirements can only be met by BMT. This notice of intent is not a request for competitive proposals, however, if an interested party believes they can meet the above specifications, they may submit a statement of capabilities. Information furnished shall include enough material, in sufficient detail, to perform a proper evaluation of their ability to perform. Closing date is 45 days after publication of this notice. SEE Note 22. (0052)

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