Centers for Disease Control and Prevention, Procurement and Grants Office, 255 East Paces Ferry Road NE, Room 405, Atlanta, Georgia 30305

B -- MOLECULAR CHARACTERIZATION OF BORDATELLA PERTUSSIS BY PULSE- FIELD GEL ELECTROPHORESIS SOL 200-95-0945(P) DUE 071495 POC Contact Susan Brandel at (404) 842-6635 The Centers for Disease Control and Prevention contemplates awarding a contract to define the molecular epidemiology of B. pertussis in North America. Pulsed-field gel electrophoresis (PFGE) will be used to type isolates. The technique will identify different genetic subtypes of B. pertussis and their geographic distribution, and allow an assessment of how prevalent strains change over time. The project will have two components: 1) to describe geographic distribution, recent B. pertussis isolates from different regions will be collected and typed; and 2) to assess temporal changes and trends in strains, a large number of isolates collected over a long period of time (5-10 years or longer) in several geographic locations will be typed. TO BE ELIGIBLE FOR THIS CONTRACT, OFFERORS MUST BE IN POSSESSION OF, OR HAVE DOCUMENTED ACCESS TO, MORE THAN 200 B. PERTUSSIS STRAINS ISOLATED IN A DEFINED GEOGRAPHIC AREA OVER A PERIOD OF 5 TO 10 YEARS, OR LONGER. The scheduled issue date is approximately May 26, 1995. NO TELEPHONE REQUESTS WILL BE ACCEPTED; only written requests for this solicitation will be honored. Written requests should cite RFP No. 200-95-0945(P). All responsible sources that meet the above requirements may submit a proposal which shall be considered by the Agency. (0121)

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