NIH, National Heart, Lung, & Blood Institute, Procurement Section, Contracts Operations Branch, Rockledge Building (RKL2), Room 6143, 6701 ROCKLEDGE DR MSC 7902, BETHESDA MD 20892-7902

66 -- LAMBDA FLUOROSCAN TRAY SYSTEM SOL NHLBI-HL-P-95-111 POC Kathleen J. Van Steelant, Contract Specialist, (301) 435-0366. It is the intent of the National Heart, Lung, and Blood Institute to negotiate on a sole source basis under the authority of 41 USC 253 (c)(1) and FAR 6.302-1, with One Lambda, Inc. to purchase a Lambda Fluoroscan Tray System for use in the Bone Marrow Transplant Rsearch Laboratory in Bethesda, Maryland. The system must meet the following minimum specifications: 1) The microscope stage shall be motorized to accommodate Terasaki trays. The stage shall accommodate different types of Terasaki trays, i.e., high and low profile, tower trays, with 60, 72 or 96 wells per tray; 2) There shall be a tray stacking system so that up to 10 trays can be loaded at one time; 3) Automated Fluoro Module - the fluorescence detection device shall be space saving in design, have a short suggestion scan time (about 15 seconds per tray) and simultaneously read both green (Calcein-AM) and red (ethidium bromide) fluorescence emission. It must be compatible with inverted fluorescence/phase contrast microscopes; 4) The fluorescence detection device shall be integrated with the computer so all fluorescence emission is discretely measured, saved and accessible for analysis by computer using commonly available statistics programs; 5) The microscope shall have a camera port appropriate for recording on film appropriate fluorescent wells; 6) Software - the software must allow different reading configurations of the trays to be analyzed, e.g., every other row or half of each tray, etc. The software must allow for input of experimental conditions, tray layouts from different manufacturers and look-up options to view subject information, tray layouts, reaction data and results. The software must allow the assignment of positive control wells, negative control wells, number of replicates, and number of repeats. The software must determine the mean and standard deviation of the replicates. It should also calculate the background and determine the cutoff value for limiting dilution assays. Then each well of the test should be analyzed by computer as negative or positive based on the cutoff value. Finally, the software should be able to calculate the precursor frequency using the Poisson distribution (maximum likelihood method). After analysis, the software should display the image of the trays for each experiment with the positive wells highlighted. Network compatibility is necessary so that data can be analyzed by one scientist while another is actually using the system to accumulate data. Security is necessary since this will be a multi-user system. Separate user names are needed to limit access to certain areas of the software; and 7) Report generation should be flexible depending on the purpose of the report. It must be possible to export the data to a file format compatible with common spreadsheet and statistics programs, e.g., Quattro Pro, SigmaPlot, etc. Installation of the system shall be accompanied by immediate on-site training. Interested parties may identify their interest and capability to respond to the requirement or submit proposals. This notice of intent is not a request for competitive proposals. However, all proposals received within forty-five (45) days after the date of publication of this synopsis will be considered by the Government. A determination by the Government not to open the requirement to competition based upon responses received as a result of this notice of intent will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. Oral communications are not acceptable and will not be honored. Interested parties may express their concerns in writing. Written responses must contain clear and convincing documentation, technical information and descriptions demonstrating the ability to meet the stated requirement. All responses must be received within forty-five (45) calendar days from the publication date of this notice and should be directed to Kathleen J. Van Steelant at the above address. Responses received by facsimile will not be honored. See Numbered Note(s): 22. (0159)

Loren Data Corp. http://www.ld.com (SYN# 0253 19950609\66-0001.SOL)