National Institutes of Health, Division of Procurement, AB-B, 6120 Executive Blvd., Room 868, Rockville, Maryland 20892

66 -- BIOSTAT Q AND BIOSTAT B SOL 263-95-B(BC)-0138 POC Sheri Lawson, Contracting Officer (301) 402-3065 or Edward Wilgus, Chief, AB-B (301) 402-3070. A solicitation will be issued for a Biostat Q and a Biostat B, benchtop fermentation system (from B. Braun Biotech), or equal. The fermentation systems shall be in accordance with the following specifications:1) Shall be a Biostat Q Multi-Fermentor-System with the following minimum required features: Supply cabinet, Gas flow rotameters, Brushless D.C. motors, Autoclavable stainless steel trays with carrying cage for two (2) culture vessels and four (4) storage bottles (two for each vessel), 1000ml glass culture vessels, pH electrode complete for 1000ml vessel - (shall be for a 12mm diameter headplate port, shall be 200mm long and have an Ingold gel filled electrode and connecting cable to supply cabinet, shall include pH 4, 7, and 9, 250ml buffer solutions), pO2 electrode complete for 1000ml vessel - (shall be for a 12mm diameter headplate port, shall be 220mm long and be a polarographic Ingold electrode with a plug connection, shall include a headplate fitting, connecting cable to supply cabinet and a membrane kit for repair/replacement of membrane), Watson Marlow peristaltic pumps for liquid additions - (shall be mounted on front panel and be for acid and alkali, shall be installed in the supply cabinet of the Biostat Q and have a flow rate (with 2 X 4 mm tubing) of 8mL/min), Digital measurement and control unit (DCU) - (shall include slots for four (4) measurement boards and four (4) VME bus boards. Shall include DCU firmware that will provide measurement and control of temperature, stirrer speed, pH and pO2 and storage of process parameters in up to four (4) recipes. DCU boards and amplifiers - (measurement M1 idle board, equipped with a temperature amplifiers (measurement range of 0 - 150oC, for connection to a Pt 100 sensor), pH amplifiers (measurement range of 2 -12 pH, for connection to an Ingold pH probe (or equal)), pO2 amplifiers (measurement range 0 - 100% sat, for connection of an Ingold pO2 probe (polarographic)). Shall be a Biostat B with the following minimum required features: Addition agent pumps shall be for acid, alkali, anti-foam and nutrient feed, shall be installed in supply cabinet of Biostat B, shall have a configuration program that assists each pump to operate defined function, shall have a Watson Marlow peristaltic pump with acid/alkali resistant head, flow rate for 2 X 4 mm tubing shall be 8 mL/min and 30 mL/min for 4 X 7 mm tubing. A pH electrode, complete, shall be provided for the B-5 vessel as follows: shall be for a 12mm diameter headplate port, shall have an Ingold gel filled electrode, insertion length shall be 317 mm and have a connecting cable to supply cabinet (100 mm), shall include a headplate fitting and pH 4, 7, and 9, 250ml buffer solutions). A pO2 electrode, complete, shall be provided for the B- 5 vessel as follows: shall be for a 12mm diameter headplate port, shall be a polarographic Ingold electrode with a plug connection, insertion length shall be 312 mm, shall include a headplate fitting, shall include a connecting cable to supply cabinet (100 mm) and a membrane kit for repair/replacement of membrane). A foam sensor, complete, shall be provided for the B-5 vessel as follows: shall be for a 6mm diameter headplate port, insertion length shall be 80 mm, shall have ceramic insulation and shall include a connecting cable to supply cabinet (100 mm). A level sensor, complete, shall be provided for the B vessel as follows: shall be for a 6mm diameter headplate port, insertion length shall be 120 mm, shall have ceramic insulation and shall include a connecting cable to supply cabinet (100 mm). Digital measurement and control system (DMCS): shall be for connection of a Pt 100 temperature sensor and have a measuring range of 0 - 150oC, shall have pH measurement and control for an Ingold pH electrode and have a measuring range of 2 - 12 pH, shall have pO2 measurement and control for a polarographic Ingold pO2 electrode with a measuring range of 0 - 100% saturation, shall have foam measurement and control for connection of a conductive foam sensor and a limit switch with digital output signal, shall have level measurement and control for connection of a conductive level sensor and a limit switch with digital output signal. Shall have standard firmware DMCS Version 1.1 with the following functions: process delays with simultaneous display of four (4) process variabled per screen, measurement and control of: temperature, stirrer speed, pH, pO2 and antifoam. Solicitation will be issued on or about 15 calendar days after publication with closing date 30 calendar days after issue date. (0181)

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