NATIONAL INSTITUTES OF HEALTH, NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES (NIDDK) NATCHER BUILDING 45 CENTER DR. MSC 6600 ROOM 6AN32 BETHESDA, MARYLAND 20892-6600

66 -- 66-PHOTOMETRIC PXL 37, HIGH SPEED MODULAR CHARGED COUPLED DEVICE COOLED CATHODE DETECTOR (CCD) CAMERA SOL NIH-NIDDK-95-110 DUE 082295 POC LIVIE V. SPEARMAN, III., CONTRACT SPECIALIST (301) 594 - 8855. The National Institutes of Health (NIH), National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) intends to purchase The PHOTOMETRIC PXL 37, HIGH SPEED MODULAR CHARGED COUPLED DEVICE COOLED CATHODE DETECTOR (CCD) CAMERA, to work together with our existing imaging system built around a Zeiss Photomicroscope III Microscope & including a 486/66/EISA computer, Universal Imaging Metamorph software, Ludle dual filter wheel, Ludle microscope stage & Z focus drivers & Panasonic optical disc recorder. The image acquisition must be fast enough to send 2-4 pictures/second to the computer to detect cellular events within seconds, work with fluorescent labels below 450 nm. Any adaptor to the microscope should be included. On site integration, testing, training and technical support is essential to ensure uninterrupted operation & to avoid unacceptable downtime with ongoing experiments. The camera has to be sensitive enough and work with low noise to allow using physiological, low dye concentrations. The transfer speed and readout time must be minimized between image pairs for reliable measurement of ratio images. To detect rapid events at low light level with our existing microscope imaging system. The camera made by Princeton Instruments has low noise level, high speed & fast transfer time necessary for our experiments. The Justification For Other Than Full & Open Competition is that the drivers for the camera must be completely integrated with the existing Universal Imaging Metamorph software to allow integrated control of the experimental conditions. The intended source Universal Imaging Corporation is the manufacture of the Metamorph software that is customized for the PHOTOMETRIC PXL 37, HIGH SPEED MODULAR CHARGED COUPLED DEVICE COOLED CATHODE DETECTOR (CCD) CAMERA, & PHOTOMETRIC CAMERA SOFTWARE DRIVERS. Universal Imaging Corporation will install and guarantee complete integration of standard software and hardware into the Universal Imaging current system, for those elements that they provide. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. (See Note 22). It is anticipated that the Request For Quotation (RFQ) Number NIH-NIDDK-95-110 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due ten (10) days after the release of the RFQ. The anticipated award date is August 22, 1995. Requests for copies of the RFQ must be in writing and maybe requested by FAX (301) 480 - 4226. Quotations must be submitted in writing to the National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) The Natcher Building 45 Center Drive MSC 6600 Room 6AN32 Bethesda, MD 20892-6600 Attention: Livie V. Spearman, III. FAX quotations will only be accepted if dated and signed by an authorized company representation. (0207)

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