National Institute of Environmental Health Sciences, Contracts and Procurement Management Branch, OM, 79 T.W. Alexander Drive, 4401 Building, P. O. Box 12874, Research Triangle Park, NC 27709

A -- GENETIC MONITORING OF RODENTS SOL NIH-ES-95-33 DUE 012396 POC Contact Vondia C. Malone, Contract Specialist, (919) 541-0428; Phillip D. Jones, Contracting Officer, (919) 541-0426 The National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), is soliciting proposals from offerors having the capability for the genetic monitoring of inbred rodents. The objective of this project is to determine the genetic integrity of rodents used in the National Toxicology Program (NTP) chemical exposure studies. The Contractor will be required to monitor up to twenty designated loci for each strain or hybrid by electrophoresis of erythrocyte lysates: kidney, liver, pancreas and lung homogenates and serum proteins. Immunochemical methods may be employed. The Contractor will receive up to 700 live inbred rodents per year at a rate of about 20 mice and 10 rats at approximately two-week intervals (40 mice and 20 rats per month) for genetic monitoring by biochemical procedures. In addition, frozen tissues (usually kidneys) from approximately 200 mice/year (kidneys from 20 mice/month) will be shipped to the Contractor by the NIEHS/NTP toxicology testing laboratories for genetic marker isozyme analyses. Based on the availability of methods, other special strains of rodents such as transgenic animals may be included for genetic monitoring. The Government estimates that .45 professional person years and .75 technical person years will be required per year. The period of performance will be five years. Request must reference RFP No. NIH-ES-95-33 and must be directed to the office listed above. (0270)

Loren Data Corp. http://www.ld.com (SYN# 0002 19950928\A-0002.SOL)