NIH, OPM, 6120 Executive Blvd, Rockville, MD 20892-7260

A -- GROW HELA CELLS AND PREPARE NUCLEAR AND WHOLE CELLS SOL 263-96-Q(CS)-1577 DUE 120995 POC Mrs. Aretha T. Latimer, Purchasing Agent, Small Purchases Operation Branch, 6120 Executive Blvd., EPS/849C, Rockville, Maryland 20892. 8734 Professional Service: RFQ-263-96-Q(CS)-1577, Poc, Purchasing Agent Arethat Latimer (301) 496-3340. NIH Intends to procure on a sole source basis unde the authority of FAR 6.302-1 for the following service: Grow Hela Cells and Prepare Nuclear and Whole Cell Extracts For In Vitro Transcriptio. Hourly Rate: This Requirement is for the Laboratory of Molecular Growth Regulation, Unit on Eukaryotic Transcriptional Regulation. NICHD the Sections purpose is to understand the mechanism of action by which sequence specific transcriptional sctivators regulate transcription by interacting w/RNA polymerase transcription machinery. The purpose and objective of this requirement is to establish an LMGR central facility to grow hela cells and prepare extracts for in vitro transcription. The contractor will establish a system to grow hela cells and prepare extracts for in vitro transcription, thus a very strong background in this field is a prerequisite for this this requirement. (0312)

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