NIH, OPM, 6120 Executive Blvd., Rockville, Md. 20892-7260

A -- GROW HELA CELLS AND PREPARE NUCLEAR AND WHOLE CELLS POC Aretha T. Latimer, Purchasing Agent, 301-402-3340. National Institutes of Health intends to procure on a SOLE SOURCE basis under the authority of FAR 6.302-1 for the following service: grow hela cells and prepare nuclear and whole cell extracts for in vitro transcription. Hourly Rate: This requisition is for the laboratory of molecular growth regulation, unit on eukaryotic transcriptional regulation. NICHD, the section's purpose is to understand the mechanism of action by which sequence specific transcriptional activators regulate transcription by interacting w/RNA Polymerase transcription machinery. The purpose and objective of this requirement is to establish an LMGR central facility to grow hela cells & prepare extracts for in vitro transcription. The contractor will establish a system to grow hela cells and prepare extracts for in vitro transcription, thus a very strong background in this field is a requirement for this requisition. See Numbered Note(s): 22. (0324)

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