National Cancer Institute, Research Contracts Branch, PSCS, 6120 Executive Blvd, EPS/Room 638, MSC 7228, Bethesda MD 20892-7228

66 -- CONFOCAL IMAGING SYSTEM WITH KYPTON/ARGON LASER SOL NO2-BC-66206-83 POC Sarah Payne, Contracting Specialist or Janet Mattson, Contracting Officer, 301-402-4509. The National Cancer Institute requires delivery and installation of a Confocal Imaging System including Kypton/Argon Laser. The Confocal Imaging System shall meet or exceed the following: 1) Shall be capable of scanning, point detecting laser scanning, confocal imaging and adapt to an existing Nikon Optiphot-2 microscope. 2) Shall have a single laser that will allow imaging at multiple wavelengths including 488nm, 568nm, and 647nm. This shall include a filter set to optimize separation of these, three wavelengths each permitting 10% or less excitation in competing channels. 3) Shall provide high speed digital photon counting for low intensity specimens and enhanced photomultiplier tubes for high sensitivity. 4) Shall provide for ease of use in multi-user laboratories because gain, black level, aperture, neutral density filters, excitation filters and emission filters, are computer controlled. Computer shall provide automatic set up for a given experiment from preset methods with one click of an icon. 5) Shall provide variable scan speeds, real time image averaging, line scanning mode, optical zoom capability, and fiber optic cable delivery of the laser line. 6) Shall be compatible with peripheral components such as printers, optical drives and workstations that may be added in the future as the use of the system grows. 7) The contractor shall insure that the existing microscope is laser safe prior to initial startup. 8) Shall have 8 bit color resolution minimum per channel and permit overlaying of images with 24 bit resolution. 9) Shall have kalman averaging to increase sensitivity and reduce background. This shall permit real or near real time visualization of the collected images. 10) The disk drive shall be rewritable, and store up to one and half gigabytes per 5 1/2 inch disk with a SCSI interface compatible with DOS, Windows, and OS/2. 11) Shall have a minimum of three sensitivity enhanced photomultiplier tubes having computer controlled, continuously variable confocal aperture which shall allow for simultaneous display of three fluorophores and a live merged image of the three fluorophores on a 21 inch monitor with a resolution of 1280 X 1024 X 24 bits. 12) Shall include integrated volume rendering software that shall manipulate 24 bit data sets and provide quantitative analysis of the data sets. The software shall be able to rotate and tilt images around any angle and be able to slice through data sets at an oblique angle and also provide features such as variable opacity renderings and a seed fill function. 13) Shall have a computer controlled sequential collection mode to eliminate bleed through and overlapping a detector channel. 14) Shall have the option of being adaptable to other research grade upright or inverted microscopes from various manufacturers to provide future flexibility. 15) Shall include acquisition and analysis software that runs 32 bit megabytes of RAM and 1 gigabyte of hard disk space. All responsible sources may submit a bid which shall be considered by the agency. The solicitation will be issued on or about February 12, 1996. Closing will be 30 days after issue date. (0019)

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