U. S. Department of Commerce, NOAA, Central Administratrive Support Center, 601 East 12th St., CC31:RFA, Rm. 1756, Kansas City, Missouri 64106

66 -- LABORATORY EQUIPMENT SOL RFQ - NFFN2800600348RA DUE 020296 POC Ronald F. Anielak (816) 426-7267 extension 226 The National Marine Fisheries Service, SEFC Charleston Laboratory, Charleston, South Carolina has a need for a GEL IMAGING AND ANALYSIS SYSTEM. GENERAL:A) gel imaging system must run in a networked UNIX environment under one of the following operating systems: Solaris 2.x(Sun OS 5.4) or Silicon Graphics Irix 5.3. B) The new system must use X-windows displays so that it may be employed from remotely networked X-terminal servers. C) The system shall be capable of analyzing one-dimensional electrophoretic gels, two-dimensional electrophoretic/isoelectric focusing gels, and DNA/RNA sequencing gels. It shall provide for the convenient export of these images and the analyses performed thereon to a data base. D) Installation and on-site training for the system shall be provided. E) Technical service shall be provided within 24 hours of request. SPECIFIC: A) Hardware, 1) The imaging system shall provide a camera with a resolution of at least 1000 X 1000 pixels. 2) The field of view covered by the camera shall be variable up to a maximum of 24 CM X 24 cm. 3) The camera shall be controlled by the software and include a parallel interface card and cable connections to Sun SPARCstation platforms. B) Software, 1) The system shall contain image acquisition software compatible with the above camera specifications and UNIX environment. 2) The system shall provide data files in a readily exportable form for incorporation into other data base and analysis programs. 3) The system shall be capable of handling multiplexed images (i.e., multiple, superimposed images of the same gel each containing different information). 4) The system for electrophoretic gel analysis shall be capable of comparing unknown to known references of molecular weight, nucleotide base length, or isoelectric point and calculating the molecular weight, fragment length, or isoelectric point of an electrophoretic band based on these standards. 5) The system shall be capable of quantifying the amount of material in a band (for example, as represented by the density of that band).6) The DNA sequencing software shall permit automatic and manual lane detection, automatic band detection, and automatic lane registration to adjust for gel distortion. Such software shall also permit editing by the user. 7) Reports for sequences should be exportable to data bases including GenBank, EMBL, Intelligenetics, GCG, Wisconsin, and MacVector. Delivery 45 calendar days ARO. FOB Destination. Only written requests to receive a copy of RFQ number NFFN2800600348RA will be accepted and shall include a point of contact, phone number, fax number, and mailing address. Facsimile requests to 816-426-7530 will be accepted; however receipt by the government is the responsibility of the sender. If there are no affirmative responses, a purchase order will be issued to Bio Image, Ann Arbor, MI. All responsible sources may submit an offer which shall be considered by the agency. (0019)

Loren Data Corp. http://www.ld.com (SYN# 0262 19960122\66-0002.SOL)