National Library of Medicine, Office of Acquisitions Management, Building 38A, Room B1N17, 8600 Rockville Pike, Bethesda, MD 20894

A -- PRODUCTION OF ANTIBODIES TO NEW CRANIOFACIAL GENES RELEVANT TO ANOMALIES AND ORAL CANCERS SOL NLM 96-100/MLM DUE 041596 POC Lynn Montalvo, Contract Specialist/Patricia S. Page, Contracting Officer, (301) 496-6546. The National Institute of Dental Research (NIDR) has a requirement to generate a library of antibody reagents for the characterization of novel genes expressed in teeth and craniofacial tissues during embryonic development. Polyclonal antibodies are to be produced in rabbits against purified proteins obtained from selected cDNA clones expressed in E. coli by relatively standard methods. The antibodies are to be evaluated by Western immunoblot analysis and immunocytochemistry for their ability to react with embryonic craniofacial tissues. The project's goal will be to produce a library of approximately 100 characterized antibodies against these novel genes. The Contractor must have experience in recombinant DNA technologies and must demonstrate that they have successfully generated polyclonal antibodies against recombinant proteins previously. The laboratory must be certified by the Federal government as meeting Good Laboratory Practice Regulations (Title 21 of the U.S. Code of Federal Regulations, Part 58) and must be accredited by the American Association for the Accreditation of Laboratory Animal Care (AAALAC) with an assurance statement on file with the Office for Protection from Research Risks (OPRR). It is anticipated that a three-year, cost reimbustement (completion) type contract will be awarded. Request for Proposal (RFP) No. NLM 96-100/MLM will be available approximately fifteen calendar days from publication of this announcement. All responsible sources may submit a proposal which will be considered by the NIDR. Copies of the RFP may be obtained by writing to the National Library of Medicine, Office of Acquisitions Management, 8600 Rockville Pike, Building 38A, Room B1N17, Bethesda, Maryland 20894, Attention: Lynn Montalvo, Contract Specialist. Telephone and fax request will not be accepted. (0029)

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