National institutes of Health; Office of Purchasing and Contracts/OD; 6006 Executive Blvd., Suite 311, Rockville, MD 20892-7058

99 -- EVALUATION OF GENES SOL 263-96-Q-(M5)-0006 DUE 032796 POC Contact Points; Purchasing Agent, Sabrina Lindsay or Contracting Officer, Clarence Jackson, on 301-496-3704. At the Nuclear Medicine Department, we are developing a new approach to targeting eukaryotic genes using 125I-labeled triplex forming oligonucleotides (125I-TFOs). Upon binding to the target sequence in the genome 125I-TFOs introduce double-stranded breaks into intracellular DNA. The accumulation of double-stranded breaks in genomic DNA could result in deletion of the target sequence or have such severe consequences as the induction of apoptosis. The mouse c-Ki-ras protoongene provides an ideal target for evaluation of 125I-TFOs. The promoter of the c-Ki-ras gene contains a suitable target, a 32-bp-long homopurine-homopyrimidine stretch, which is vital for gene expression. A convenient model for cellular studies is the mouse cell line, Y1, where the c-Ki-ras gene is amplified approximately 50-fold, leading to dramatic target enrichment. Must perform the following experiments: 1. Design of radiolabeled purine and pyrimidine rich TFOs which bind to c-Ki-ras promoters most strongly & specifically, causing efficient double stranded breakage of DNA. 2. An analysis of 125I-TFOs functioning in a cellular environment. 3. Use 125I-TFOs against the c-Ki-ras promoter which demonstrated the strongest effect in the experiments described above will be delivered into the Y1 cells by either lipofection or electroporation. Therefore, it is anticipated that the only source capable of meeting this requirement is the University of Illinois, Chicago. However, any vendor wishing to submit an offer should contact Sabrina Lindsay, Purchasing Agent, 301-496-3704 at the above address. Any bids must be received by close of business 3/27/96. (0045)

Loren Data Corp. http://www.ld.com (SYN# 0472 19960215\99-0001.SOL)