NIH, National Heart, Lung, and Blood Institute, COB, Procurement Section, 6701 Rockledge Drive MSC 7902, Bethesda, MD 20892-7902

66 -- ELECTRON MICROSCOPE SOL NHLBI-PS-SP-96-204 DUE 032696 POC Deborah Coulter, Purchasing Agent, (301) 435-0366 Debra Hawkins, Contracting Officer, (301) 435-0366. The National Heart, Lung, and Blood Institute intends to negotiate with Philips Electronic Instruments, 6440-A Dobbin Rd, Columbia, MD 21045, to purchase a one (1) each, Slow-Scan CCD Camera PT #694/10B-2 and one (1) each, CRYO Vacuum Control PT #PW6474/00 for a Philips CM120 Twin Electron Microscope. The instrument must be capable of supporting imaging, diffraction, electron energy loss spectroscopy, cryo-electron microscopy, x-ray microanalysis, in with fixed beam and scanned beam modes. The unit must incorporate dedicated microprocessor control of all models of operation, including control and memory of all electron optical and alignment parameters. The electron optical column must be factory aligned with no mechanical parts except apertures. Must provide a fully motorized 5-axis, side entry specimen stage with +70 degree tilt. Specimen drift should be less than 0.3 nm per minute. Specimen holders must be available for rapid specimen loading, and cryotransfer. Specimen insertion must be through an automatic pre-pumped airlock with high tension remaining on during specimen exchange. Vacuum should be (2.7.x10-5 Pa, with partial pressure of water vapor (8x10-6 Pa. Specimen area, gun area and column down to the final projector lens must be pumped by an oil-free pump and not by an oil diffusion pump. A pressure differential of 104 must be maintained between the plate camera and the column with a differential aperture of diameter (0.2.mm. Accelerating voltage should go up to 120 kV in steps of 20Kv. The instrument must provide operation of a LaB6 source. All column alignments should be carried out with electromagnetic beam deflectors. There should be four illumination lenses: condenser 1, condenser 2, mini-condenser, and objective- condenser. Illumination should stay constant independent of magnification. The minimum TEM spot size should be 2nm diameter. In all operating models the specimen must be in the eucentric plane and at the optical and mechanical center of the symmetrical objective lens. The spherical aberration constant should be 2mm. The chromatic aberration constant should be 2mm. Electrical stability should be (2 ppm/minute at 120 kV. Smallest focal step should be 3nm. Resolution should be 0.2nm line and 0.34 nm point. Objective lens should have constant excitation from 400x to 450,000x magnification. Imaging system should consist of five lenses: objective, diffraction, intermediate, projector 1, projector 2. Magnification range should be from 50x to 750,000x controlled from a single knob. The camera length for diffraction should be variable from 30 to 5000 mm in the selected area mode and up to 2000 m in the low angle mode. Film camera should record images on 3.25''x4'' film with at least 50 exposures. Camera control should be completely electronic. Instrument should supports STEM operation. Instrument should support energy-dispersive x-ray microanalysis with low background and with at least 0.13 steradians collection soild-angle. Instrument should support retractable cryo anti-contamination blades so that both energy-dispersive x-ray microanalysis with 0.3 steradian solid-angle collection and contamination-free cryoelectron microscopy are possible on the same instrument without breaking vacuum. Slow-scan CCD camera must have a fiber optic coupling to a single crystal YAG scintillator providing a detective quantum efficiency ) 0.5 for single electron detection. Slow-scan CCD camera must be compatible with image acquisition using Gatan Digital Micrograph software and compatible with the Gatan Imaging Filter Spectrometer. It is anticipated award date of 4/1/96. This not a request for competitive proposal. However all responses received before the closing date (3/26/96) of this advertisement will be considered by the government. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. Responses must be submitted in writing to the National Heart, Lung, and Blood, Institute, Contracts Operation Branch, Procurement Section, Rockledge Building, Room 6143, 6701 Rockledge Drive, Bethesda, MD 20817-7902, Attention: Ms Deborah Coulter. FAX responses will only be accepted if dated and signed by an authorized company representative. See Numbered Note(s): 22. (0072)

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