Naval Surface Warfare Center, Carderock Div., White Oak Site, 10901 New Hampshire Avenue, Silver Spring, MD 20903-5640

A -- RESEARCH IN BIOCORROSION, BIOFOULING, ENVIRONMENTAL MICROBIOLOGY, BIOMARINE ENGINEERING, AND BIOSENSORS. SOL N00167-96-R-4036 DUE 072496 POC Linda Folk, Code 3324, Contract Specialist (301) 394-3780, Renee Cairo-Iocco, Contracting Officer, or Pamela Collins. The Carderock Division, Naval Surface Warfare Center (NSWCCD) intends to negotiate on a sole source basis with the University of Maryland, Center of Marine Biotechnology (COMB). This procurement is being pursued as a sole source follow-on effort to prior research conducted in conjuction with COMB, and unacceptable delays in the completion of this research would result if other sources were considered at this time. The Biotechnology Laboratory at NSWCCD in Bethesda, MD, has a requirement for a fully equipped facility with expertise in microbial attachment (biofouling and biocorrosion), molecular biology, microbial products, (i.e., bioemulsifiers, biosurfactants, lipases, esterases, etc.), applied and environmental microbiology including bioremediation, protein pattering for biosensors, growth and maintenance of marine animals and plants, and acoustic effects on marine organisms. The facility must have: (1) facilities for the production of polyclonal and monclonal antibodies; (2) equipment and expertise for fluorescent labeling studies; (3) an anaerobic atmospheric hood; (4) expertise in microbial physiology and microbial genetics for marine microbes, soil microbes and freshwater microbes (aerobes, facultative anaerobes and strict anaerobes); (5) HPLC, GC/MS, and NMR equipment; (6) equipment and expertise for molecular biology experiments including DNA extractions, restriction digests, agarose and polyacrylamide gel electrophoresis, nick translation, Southern Blots and Western Blots; (7) facilities for growth of marine animals and plants, including finfish. Close contact with the researchers at NSWCCD will be essential; therefore, weekly reports via the telephone and monthly meetings at NSWCCD are required. The contract is good for a period of three years. See numbered note 22. (0145)

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