National Cancer Institute, Research Contracts Branch, PSCS, 6120 Executive Blvd, EPS/RM 634 Bethesda, MD 20892-7228

B -- ESOPHAGEAL CANCER GENETIC STUDIES SOL N02-CN-66211-81 POC Janet M. Mattson, Contracting Officer (301)402-4509. The National Institutes of Health plans to procure on a Sole Source Basis, under authority of FAR 6.302-1, in accordance with 41 U.S.C. 253 (c)(1), a genetic study on esophageal cancer from Shanxi Cancer Hospital, Ahigongxinjie, Taiyuana 030013, Shanxi, China. This is an ongoing study and a continuation and study of additional patients. A capability to provide samples and data to test for genetic polymorphisms to identify potential susceptibility genes associated with risk for cancers of the esophagus, gastric cardia, and gastric body is needed. Requirements include: (1) Identify 350 newly diagnosed and untreated upper gastrointestinal cancer cases (50 esophageal, 150 gastric cardia, 150 gastric body) from Shanxi Province in China going to surgery and (1a) obtain tumor and adjacent nontumor tissue from these cases during surgery, (1b) preserve portions of tumor and nontumor specimens in formalin, ethanol, and liquid nitrogen, (1c) extract DNA from liquid nitrogen preserved specimens, (1d) from each ethanol-preserved specimen, prepare one H&E stained section, microdissect the tumor, and extract DNA from the microdissected tissue, (1e) from each formalin-preserved specimen, prepare one H&E stained section, (1f) obtain 15 ml venous blood sample from each of the 350 esophageal/gastric cardia/gastric body cancer patients and extract DNA from these samples, (1g) obtain finger stick blood sample from each of the 350 esophageal/gastric cardia/gastric body cancer patients and retain samples on blotter paper, (1h) obtain buccal cells samples from each of the 350 esophageal/gastric cardia/gastric body cancer patients and extract DNA from these samples, (1i) obtain questionnaire information (general information, family history, lifestyle information, and medical abstract), (2) Obtain a 10 ml whole blood sample from 100 healthy volunteers from a Yangcheng County, Shanxi Province, China, high-risk area of esophageal cancer, and extract the DNA, (3) Identify 1400 newly diagnosed and untreated upper gastrointestinal cancer cases (600 esophageal, 600 gastric cardia, 200 gastric body) from Shanxi Province, China going to surgery, (3a) obtain tumor tissue from these cases during surgery (or endoscopy), (3b) preserve portions of tumor specimens in formalin and ethanol, (3c) from each ethanol-preserved tumor specimen, prepare one H&E stained section, microdissect the tumor, and extract DNA from the microdissected tissue, (3d) from each formalin-preserved tumor specimen, prepare one H&E stained section, (3e) obtain 15 ml venous blood sample from each of the 1400 esophageal/gastric cardia/gastric body cancer patients and extract DNA from these samples, (3f) obtain finger stick blood sample from each of the 1400 esophageal/gastric cardia/gastric body cancer patients and retain samples on blotter paper, (3g) obtain buccal cell samples from each of the 1400 esophageal/gastric cardia/gastric body cancer patients and extract DNA from these samples, (3h) obtain questionnaire information (general information, family history, lifestyle information, and medical abstract), (4) Identify 1000 comparison subjects without upper gastrointestinal cancer from the general population of Shanxi Province, China, (4a) obtain 10 ml venous blood sample from each of the 1000 comparison subjects and extract DNA from these samples, (4b) obtain finger stick blood sample from each of the 1000 comparison subjects and retain samples on blotter paper, (4c) obtain buccal cells samples from each of the 1000 comparison subjects and extract DNA from these samples, (4d) obtain questionnaire information (general information, family history, lifestyle information, and medical history), (5) Identify 150 families with 2 or more cases of upper gastrointestinal cancer in which a tissue sample is available, (5a) obtain tissue from each case to confirm diagnosis histologically and extract DNA from tissue, (5b) obtain 10 ml venous blood sample from each case and all living first degree relatives over 20 years old and extract DNA from these samples, (5c) obtain finger stick blood sample from each case and all living first degree relatives over 20 years old retain samples on blotter paper, (5d) obtain buccal cell samples from each case and all living first degree relatives over 20 years old and extract DNA from these samples, (5e) draw family pedigrees and obtain questionnaire information from each case and their first degree relatives (general information, family history, lifestyle information, and medical history). This notice of intent is not a request for competitive proposals, however, interested parties may identify their interest and capability to respond to the requirement. Information furnished shall include enough material, in sufficient detail, to perform a proper evaluation of their ability to perform. Closing date is 45 days after publication of this notice. See Numbered Note(s): 22. (0149)

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