National Institute of Standards and Technology, Acquisition and Assistance Division, Building 301, Room B117, Gaithersburg, MD

66 -- OXFORD CRYOSTREAM UNIT SYSTEM SOL 52SBNB6C9173 DUE 072296 POC Keith Kennedy (301) 975-6325/Contracting Officer, Phyllis Bower (301) 975-6336 The National Institute of Standards and Technology intends to purchase on a sole source basis, two each Oxford Cryostream unit systems, from Charles Supper Co., Natick, MA. The systems shall flash freeze protein crystals with no ice formation to temperatures in the range of from 80 to 375 K and maintain that temperature for periods of greater than two weeks. The system required for planned crystallographic studies shall: feature a cooler which shall supply dry nitrogen gas with programmable temperatures in the range from 80 to 375 K; the cooler shall also be portable and supplied with a support stand that permits users to retract the coldhead when not in use and to reposition the coldhead at the crystal in reproducible fashion; control temperature to plus or minus 0.1 K; the temperature controlling unit shall program ramps, plateaus, and combined ramps and plateaus so that NIST can perform automatic temperature versus structure studies; the temperature control unit shall also feature a built-in RS 232 interface so that NIST can interrogate or remotely control it; consume 0.6 or less L per hour; feature a flexible cryotransfer tubing of at least 130 cm connecting the cooler head and the liquid nitrogen storage vessel; the coldhead design shall supply a concentric flow of dry air to prevent frosting of the crystal. The contractor shall supply an appropriate air dryer that can provide a continuous supply of dry air with a dewpoint of less than minus 60 degrees Celsius at a flow rate of 25 L per minute. The contractor shall supply a splitter that can simultaneously provide service to two units; be of open, not pressurized, design so that users can charge the system with liquid nitrogen with no effect on the cooling parameters; must be compatible with existing Siemens and Rigatu X-ray generators, radiation enclosures and cameras. FOB destination 90 calendar days after contract award. This notice of intent is not a request for competitive proposals. NIST intends to solicit only one source under authority of FAR 6.302-1. See numbered Note 22. (0150)

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