NIH, OPM, Acquisitions Branch A, EPS, Rm 801, Rockville, Maryland 20892-7260

65 -- ATTOFLUORO RATIOVISION IMAGE SYSTEM SOL 263-96-P(LQ)-0122 DUE 072996 POC Deborah Britton, Contract Specialist, 301-402-3068. Under authority of 41 U.S.C. 253(c)(1), FAR 6.302-1, the Government intends to purchase Attofluoro Ratiovision Image System. The Attofluoro Ratiovision System shall have the following minimum required specifications: The system shall have the capability of providing high speed resolution simultaneously from up to 99 cells and shall provide dual dye technology for analysis of simultaneous video acquisition, analysis and viewing of ratio, single excitation and/or transmitted light images; shall have calibrated excitation and/or emission rationing with simultaneous background subtraction, shading correction and exclustion with up to 99 Regions of Interest (ROI) plus post-experimental analysis from stored images; shall have simultaneous or sequential multi-dye capability; shall provide no less than 30 full field image (512X480) ratios per second acquisition rates; shall have Dual Dye Technology for analysis of simultaneous dual dye experimentation such as FURA-2 and BCECF or FURA-2 and Cyclic AMP; shall have ratio arc allowing continuous electronic control of lamp intensity for UV applications; shall have a dual emission camera system providing accuracy within one pixel, utilizing precise image registration hardware and consisting of two ICCD camera systems; shall have integrated atto data analysils software (AttoGraph) which shall run on any stand alone PC; AttoGraph shall be integrated with AttoView to allow flexible image viewing from experimental data sets; Uncage and Photobleaching software functions shall be fully integrated; shall have full digital control of ICCD cameras GAIN and BLACK levels via computer; shall have capability for input of up to 8 analog channels, for electrical recordings of physiological changes; shall have integrated Macro facility for automating routines; and, host computer shall have the following minimum characteristics: pentium processor with 90Mhz clock, 3MB Video RAM, 1GB EIDE HD, 16 MB RAM, 2 serial ports, 3 parallel ports, and shall include 4 GB video rate HD to allow writing of images at 30 frames per second (up to 15 minutes shall be recorded at video rate). It is the Government's belief that these requirements can only be met by Carl Zeiss, Inc., Medical Consumer Dept., One Zeiss Drive, Thornwood, NY 10594. This is not a formal solicitation. However, concerns that respond to this notice must furnish concise responses directed specifically to the requirements mentioned above. Sources demonstrating the capability to accomplish the above, shall supply pertinent information in sufficient detail to demonstrate their ability to perform. Information furnished shall include enough material to perform a proper evaluation. Concerns submitting material may at that time request a copy of the solicitation when it becomes available. Respondents will not be notified of the evaluation results. Closing date will be 45 calendar days after publication of this notice. Note 22. (0151)

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