U.S. Dept. of Commerce, NOAA, WASC, Procurement Division, R&D Contracting Branch WC33, 7600 Sand Point Way NE, BIN C15700, Seattle, WA 98115

A -- OUTBREEDING DEPRESSION IN PACIFIC SALMON SOL 52ABNF-6-00110 DUE 081396 POC For further information, call Contract Specialist Jan Sullivan at 206-526-6032. FAX number is 206-526-6025. This publication serves as a notice of intent to negotiate a sole-source firm fixed-price contract with the State of Washington, Department of Fish and Wildlife (WDFW), to provide a suitable facility and conduct a study of out-breeding depression expressed in Pacific salmon and to determine the genetic mechanism for this expression. We believe the Washington Department of Fish and Wildlife is the only agency in Washington State that is capable of providing a suitable facility and coho salmon stocks to conduct and monitor an experiment of this scale and complexity. South of Alaska, Puget Sound coho salmon are the organism of choice for this experiment because of their nearly invariant generation time (3 years) and relatively high smolt-to-adult survival rates. Although coho salmon populations also occur in Oregon and California, we believe that this study should be implemented with Puget Sound coho salmon for two reasons: 1) most Puget Sound populations and hatcheries are readily accessible from the Northwest Fisheries Science Center, which would facilitate direct supervision by NMFS scientist; and 2) populations south of northwestern Washington are generally thought to be at increased risk of extinction and may therefore be inadequate to meet the study's sampling requirements. The Contractor shall be required to: must spawn coho salmon from three Puget Sound populations; spawn adults from these populations to yield experimental groups that are each established from at least 20 single-pair matings. The period of performance will be a base year plus five option years. Any firm wishing to offer on this procurement must show demonstrated experience necessary to perform this project. This notice may represent the only official notice of such solicitation. See Numbered Note 22. (0187)

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