Centers for Disease Control and Prevention (CDC), Procurement & Grants Office, 255 East Paces Ferry Road, N.E., Room No. 500 Atlanta, Georgia 30305

A -- MOLECULAR EPIDEMIOLOGY OF AIDS SOL 200-96-0516(p) DUE 082396 POC Contact Barry E. Smith, Contract Specialist at (404)842-6787 The Centers for Disease Control and Prevention (CDC) contemplates awarding a sole source contract to Children's Hospital of Oakland Research Institute (CHORI) for a three year study of the molecular epidemiology of AIDS. The contractor shall analyze sequences provided by CDC to determine: (a) inter-person and intra-person nucleotide sequence similarities; (b) pairwise and multiple alignments of nucleotide and amino acid sequences; (c) genetic distances between sequences; and d) phylogenetic and evolutionary analysis through genetic distance, parsimony and/or maximum likelihood trees of the new sequences with suitable known HIV-1 reference strains. In order to be considered for this contract a prospective offeror must, within 15 days of publication of this synopsis, demonstrate its capability to provide detailed and unique molecular information concerning DNA sequences derived from the envelope, gag, or other genes from HIV-1 infected persons and provided by the government. The offeror must have access to and be able to use Eyeball Sequence Editor (ESEE) version 2.00B, in order that individual sequences can be aligned, unalignable regions removed, and files with alignable regions can be created for individual sequences, as well as a sequence figure for documentation. The offeror's ability to create multi-sequence data files for each of the phylogenetic analysis programs, create distance matrices with multiple-hit correction using PHYLIP program DNADIST, format corrected distance data files and determine intra-person and inter-person nucleotide sequence diversity, perform neighbor-joining bootstrap analysis using the PHYLIP programs NEIGHBOT and FITCH in association with SEQBOOT and CONSENSE must be state-of-the-art. In addition, the offeror must have the ability to perform Parsimony analysis and bootstrapping using the Phylogenetic Analysis Using Parsimony (PAUP) version 3.0q and the maximum likelihood program DNAML. Offerors who request a copy of the solicitation and/or simply provide a statement that they can perform the work required will not be considered. In the absence of other qualified sources, it is the Government's intent to make a sole source award to CHORI. Only those responsible sources that meet the above requirements may submit a proposal which shall be considered by the Agency. See numbered Note 22. (0190)

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