National Cancer Institute, Research Contracts Branch, OC, 6120 Executive Blvd, EPS/RM 608 Bethesda, MD 20892-7222

66 -- CONFOCAL MICROSCOPE SYSTEM SOL N02-BC-66216 POC David Keefer, Contracting Officer 301/496-8628. This is a combined synopsis/solicitation for commecial items, prepared in accordance with the format in FAR 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation, proposals are being requested and a written solicitation will not be issued. The solicitation, No. NO2-BC-66216, is issued as a Request for Proposal (RFP). The solicitation/contract will include all applicable provisions and clauses in effect through FAR FAC 90-36. The Standard Industrial Clasifiction Code is 3827 and the business size standard is 500 employees. However, this requirement is not set- aside for small businesses. The laser scanning confocal microscope system shall meet the following requirements/specifications: A. The system shall have optimal configuration, integrated by the prime contract. Further, the prime contractor shall be responsible for the maintenance and technical support of the integrated system. B. The system shall have a modular design and be fully upgradeable to accommodate additional lasers and detectors. C. The system shall include a fully functional inverted microscope with extremely high resolution, high contrast optics for transmitted light and fluorescence applications. This microscope will be equipped with a direct TV or Keller video port in the base and a 35mm camera port on the front of the stand to accommodate accessory cameras without interfering with confocal operation. D. The microscope must be able to accommodate manipulators, injectors, temperature control, and atmospheric chambers while remaining accessible and easy to use. E. System shall have an internal 633nm HeNe laser and an external 220V, 488/568nm Ar/Kr laser. If the system is upgraded to have a UV laser, the UV laser must be aligned within one pixel of visible line lasers. F. The system shall have automatic, computer controller laser idling to reduce power consumption and extend laser lifetime. Additionally, it shall have redundant, automatic safety features to prevent laser transmission through microscope oculars. G. The system shall be fitted with high sensitivity, large surface area PMT fluorescent detectors, with specific detectors designed for selective sensitivity for red and green emissions. H. The system shall have individual, digitally controlled confocal apertures (pinholes) for each PMT fluorescent detector. I. The Z-focus motor should operate a +/-50nM step and shall be precisely encoded to travel +/- 1 step over the complete range of travel to make use of entire Z-imaging area of long working distance objectives. J. The confocal shall have easily changeable dichroic mirrors and emission filter sets. K. The confocal hardware shall utilize dual scanning galvanometer mirrors to allow 360 degree rotation of scan orientation. L. The confocal shall have optical correction for wavelengths from UV through near IR to ensure X, Y, and Z registration within confocal images and image series. M. The computer system shall utilize an open PC platform and ethernet connectivity hardware for easy network and peripheral intergration within the environment in use in the Experimental Immunology Branch (EIB). N. The systems software must meet the following requirements: 1. Acquisition and analysis software must run under the Windows 95 operating system which is the standard within EIB. 2. For off-line image processing, software must be able to run on PCs in the absence of attached confocal, and appropriate licensing must be available. 3. System must provide acquisition and processing functions supporting 1024x1024 pixel, 24 bit RGB, TIF, or BMP image formats. 4. System must provide sophistcated, user-friendly macro- programming facility for automated acquisition and/or analysis. 5. It must be possible to easily configure and recall machine setups for multiple different users. 6. Software shall include integrated and easy to use time series, 3D reconstruction and quantitative analysis functions, and be capable of performing on-line ratios during image acquisition. O. Proposal must include installation, user training and network installation/integration support. The confocal microscope system must have a minimum warranty period of one (1) year, including parts and labor. Delivery shall be made F.O.B Destination, no later than 120 days after contract award, to: Experimental Immunology Branch Division of Basic Sciences, NCI/NIH Building 10, Room 4509 9000 Rockville Pike Rockville, MD 20892 Offerors must provide product literature, owner's manuals, warranties, and/or other existing materials that demonstrate that their offer meets the foregoing requirements. The contract will be awarded to the responsible offeror with the lowest-price offer that fully meets to the requirements of the solicitation. This solicitation incorporates the provisions at FAR 52.212-1, INSTRUCTIONS TO OFFERORS-COMMERCIAL ITEMS, and at FAR 52.212-3, OFFEROR REPRESENTATIONS AND CERTIFICATIONS - COMMERCIAL ITEMS. The resulting contract will incorporate the requirements of the clause at FAR 52.212-4, CONTRACT TERMS AND CONDITIONS - COMMERICAL ITEMS, and the requirements contained in paragraphs (a), (d), and (e) of the clause at FAR 52.212-5, CONTRACT TERMS AND CONDITIONS REQUIRED TO IMPLEMENT STATUTES OR EXECUTIVE ORDERS - COMMERCIAL ITEMS. The contract will also incorporate the clauses at FAR 52.203-6, RESTRICTIONS ON SUBCONTRATOR SALES TO THE GOVERNMENT, with ALTERNATE I, at FAR 52.203-10, PRICE OR FEE ADJUSTMENT FOR ILLEGAL OR IMPROPER ACTIVITY, at FAR 52.219-8, UTILIZATION OF SMALL BUSINESS CONCERNS AND SMALL DISADVANTAGED BUSINESS CONCERNS, at FAR 52.222-26, EQUAL OPPORTUNITY, at FAR 52.222-35, AFFIRMATIVE ACTION FOR SPECIAL DISABLED AND VIETNAM ERA VETERANS, at FAR 52.222-36, AFFIRMATIVE ACTION FOR HANDICAPPED WORKERS, at FAR 52.222-37, EMPLOYMENT REPORTS ON SPECIAL DISABLED VETERANS AND VETERANS OF THE VIETNAM ERA, at FAR 52.225-9, BUY AMERICAN ACT - TRADE AGREEMENTS - BALANCE OF PAYMENTS PROGRAM, at HHSAR 352.202-1, DEFINITIONS, and at HHSAR 352.232-9, WITHHOLDING OF CONTRACT PAYMENTS. Offerors must submit a completed copy of FAR 52.212-3, OFFEROR REPRESENTATIONS AND CERTIFICATIONS - COMMERCIAL ITEMS, with their offer. Full text copies of these representations and certifications or other cited provisions and clauses may be obtained from David Keefer on (301) 496-8628 (voice) or (301) 480-0309 (fax). Offers must be in writing and are due no later than TWENTY- ONE (21) DAYS after publication of this notice. Please cite the solicitation number on your proposal. (0194)

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