NIH, National Heart, Lung, and Blood Institute, COB, Procurement Section, 6701 Rockledge Drive MSC 7902, Bethesda, MD 20892-7902

66 -- DELAYED EXTRACTION MATRIX-ASSISTED LASER DESORPTION MASS SPECTROMETER SOL NHLBI-HL-P-96-116 POC Kathleen J. Van Steelant, Contracting Officer, (301) 435-0364. It is the intent of the National Institutes of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI) to purchase a Delayed Extraction Matrix-Assisted Laser Desorption Mass Spectrometer with the following minimum specifications: 1) The equipment shall have negative ion capability with switching from positive to negative mode controlled by the software and automated in autosampler mode to allow the user to run positive and negative analysis during the same autosampler run; 2) The equipment shall have a computer-controlled, mass-selectable low mass gate circuit to enhance detector response through elimination of matrix ions. The software shall be able to store and retrieve the selected mass for the upper limit of this circuitry and use it in both automated and manual acquisition methods; 3) The equipment shall have a two stage ion source design for flexibility and control. The acceleration voltage gradient control available with this design allows the user to control resolution and signal to noise for different mass ranges; 4) The equipment shall be shipped with Delayed Extraction technology; 5) The equipment shall have a video camera and monitor to visualize the sample plate in real time and view the laser firing; 6) The user shall be able to move the sample in both the X and Y directions for manual and automated analysis to allow the user to search the entire spot for the best signal; 7) The equipment shall have air cooled turbo molecular pumping. All pumping hardware shall be contained within the system and not require any external compressed gas or mechanical pumping. SENSITIVITY: 1) Presence of 50 fmol of neurotensin (1673.97 m/z) in a mixture containing 1 pmol of des-arg-bradykinin, Angiotensin and Glu 1 Fibrinopeptide B with a Signal to Noise greater than 5. MASS ACCURACY: 1) External = .05%; 2) Internal = Better than .01%; 3) Must show resolution of greater than 50 for mouse Igg at m/z 149,000 daltons. In addition, the dimer must also be visible at m/z 300,000 daltons with a Signal to Noise Ratio of 3 to 1. DATA SYSTEM SPECIFICATIONS: 1) Shall have peptide digestion software for recognizing and labeling amino acid sequences; 2) The equipment shall have automation software with the following capabilities: a) User selectable search patterns or coordinated across the sample well; b) Choice of signal strength or signal to noise for data criteria; c) Choice of best spectra that meets criteria or all spectra that meets criteria; d) Method driven analysis that allows negative, positive switching within the automatic run; and e) Up to 100 samples may be accessed within a single automatic run. The Contractor shall deliver and install the Delayed Extraction Matrix-Assisted Laser Desorption Mass Spectrometer in the Laboratory of Biophysical Chemistry, NHLBI. Proposals will be evaluated based on the following criteria: 1) Resolution shall be at least 50 (M/~M 50% valley) for mouse Igg at m/z 149,000 Daltons. The dimer shall also be visible with a Signal to Noise ratio of 3 to 1. At m/z 16,952 (apomyoglobin) the resolution shall be at least 1,000; 2) Mass accuracy shall be better than 0.01% with internal standard and at least 0.05% with external standard; 3) 50 fmol sample of neurotensin must show a Signal to Noise of at least 5 when run in a mixture containing 1 pmol of des-arg-bradykinin, angiotensin and Glu 1 fibrinopeptide B; 4) Must have a data system for full instrument control and data processing, including protein data bank software. Must allow choice of best spectra for signal strength or Signal to Noise criteria and choice of best or all spectra that meets criteria. Must allow user-selectable search patterns across the sample well; 5) Must have a video camera observing the sample holder and the ability to position the laser beam anywhere in the X Y coordinate by the data system; and 6) Must be able to exclude low mass ((250) ions. A firm fixed-price type contract will be negotiated. It is anticipated that Request for Proposals (RFP) Number NHLBI-HL-P-96-116 will be issued on or about August 25, 1996. Copies of the RFP may be obtained by writing to the National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch, Procurement Section, Rockledge Building II, 6701 Rockledge Drive, Room 6143, MSC 7902, Bethesda, Maryland 20892-7902, Attention: Kathleen J. Van Steelant. (0212)

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