NIH, National Heart, Lung, and Blood Institute, COB, Procurement Section, 6701 Rockledge Drive MSC 7902, Bethesda, MD 20892-7902

66 -- CONFOCAL LASER MICROSCOPE SOL NHLBI-DA-P-96-129 DUE 092096 POC Gina M. Cianflone, Procurement Assistant, (301) 435-0366 Debra C. Hawkins, Chief, Procurement Assitant, (301) 435-0367. This is a synopsis for commerical items, prepared in accordance with the format in FAR supbart 12.6, as supplemented with additional information included in this notice. The National Institute of Drug Abuse (NIDA) of the National Institutes of Health (NIH) has a requirement to obtain a Confocal Laser Microscope equivalent to the Carl Zeiss, Inc's Model #LSM 410. The technical specification are as follows: The confocal microscope must be capable of accommodating multiple users and have the capacity for future upgrading as the need arises. It must be a versatile system optimized for ease of use and maintenance. Specifically, it must include the following features: Shall be capable of accepting up to four simultaneously attached laser light sources such as, An air-cooled Argon laser with emission lines at 488nm (blue, flourescein) and 514nm (which is sub-optimal for rhodamine excitation and will also excite rhodamine.) This laser should be fiber optically coupled to the scan head to prevent erroneous sample excitation due to laser wander that may occur in mirror coupled systems, A convection cooled Dual Helium Neon laser with excitation line at 543 (green) and 633 (red). This laser should be fiber optically coupled to the scan head to prevent erroneous sample excitation due to laser wander that may occur in mirror coupled systems, An Ultraviolet Argon with lines at 351-365 nm that is not fiber optically coupled directly to the scan head, The microscope must be inverted and equipped for conventional epifluorescence and DIC microscopy. The nosepiece must have 6 places and be motorized, The confocal detectors shall be equipped with independently digitally controlled and reproducible pinholes. The confocal system shall be capable of displaying images in 24 bit RGB at 8 bits per channel, Transmitted and reflected images may be overlaid in the three channels, The system shall allow for simultaneous three channel laser line scanning and detection, The confocal system must incorporate a Z axis focus motor with 50nm + one step resolution, Z axis focus shall use a DC-servo optical encoder for position readout, Z axis focus motor shall be attached directly to the focus shaft of the microscope (9.9mm total travel distance). This allows for high resolution, better reproducibility and an increased working distance, The microscope must include the following objectives: 10x eyepiece, 40x C-APO, 1.2 NA with a 220 micron working distance and an adjustable cover slip correction from 0.14 to 0.18mm allows for good transmission in the UV sector. The lens provides a depth of field of 1 ray length unit = 0.41 mm at 546nm, and a 4.13 mm focal length. The correction collar carries 2mm divisions going beyond the minimal range from 0.13 to 0.19 mm. The lens offers correction for temperature varied samples at either 23 or 37 C, The system shall be controlled by a Pentium computer running under Windows 3.11, Image size shall be user selectable up to 1024x1024 with any rectangular frame size specified with a user display selectable to 1024x1024, The system shall be capable of scanning a 512x512 image in 0.5 seconds to 64 second scan time, The system shall have full digitally operated motorized control of laser attenuation and wave length selections., The system shall provide computer controlled 1:8 optical zoom, The system shall have a full digitally controlled and reproducible contrast and brightness settings, system shall have the capability to autoscan, contrast, brightness for use to find optimal images, The system shall include capability of interpretive level, user-defined macro routines within the standard software, The system shall include 3-D reconstruction software to produce red-green anaglyph pairs, 3-D projections and full time series. The system shall collect time base kinetic data in both 2-D and 3-D modes., The system shall include complete air table which supports and isolates microscope body and all lasers form vibrations., The system include an upgradable ultra-violet Argon laser that shall be aligned and corrected for precise overlay (+ 1 pixel) with emission form visible lasers and correction shall be specific to each objective., The system shall accept direct video input form standard video cameras and input shall be fully compatible with full software package as provided.,Images shall be stored in TIFF format., The laser shall be able to scan 1x1 pixel., The system shall be a complete laser microscope workstation fully compatible with the following simultaneous techniques: Laser Tweezers, Titanium Sapphire for two photon excitation, Laser Oblation, The system shall include all LSM filters for simultaneous excitation transmission of visual fluorochromes which include minimally: Lucifer Yellow, Acridine Orange, Tetramethyl Rhodamine, Texas Red, Cy-3, Cy-5, Fluorescence Green Protein, Bodipy, Band pass filters shall be provided that prevent bleed through for simultaneous excitation FITC, Texas Red, Cy-3, Cy-5 and similar combinations. Delivery, installation, set-up, and initial training is anticipated to be within 4 months after receipt of the contract. The contractor will provide a minimum of 4 working days of on-site training at no additional cost to the government. The contractor shall be responsible for coordinating, with the Project Officer, the schedule of training. This solicitation is not a set aside. The applicable Standard Industrial Classification (SIC) is 3827, size standard 500 employees. The proposals may be due fifteen calendar days from the publication date of this synopsis. Interested parties should submit a written request (via mail or facsimile transmission to (301) 480-3345) for a copy of the solicitation number NHLBI-DA-P-96-129 to the National Heart, Lung, and Blood Institute, Contracts Operations Branch, Procurement Section, Building RKL2, Room 6143, 6701 Rockledge Drive, Bethesda, Maryland 20892-7902, Attention: Gina Cianflone. All responsible sources may submit a proposal, which if timely received shall be considered by the agency. See Numbered Note(s): 12. (0249)

Loren Data Corp. http://www.ld.com (SYN# 0250 19960906\66-0002.SOL)